Monitoring the Dutch Phytophtora infestans population for virulence against new R-genes

Research output: Chapter in Book/Report/Conference proceedingConference paperAcademic

Abstract

New possibilities offered by marker assisted breeding and GMO breeding have sparked renewed international efforts to breed for durable potato late blight resistance. Phytophthora infestans is however known for its adaptability, a trait confirmed by recent discoveries on the structure of the P. infestans genome. One of the possibilities to enhance the durability of newly introduced host resistance is to monitor the pathogen population for virulence to new R genes, prior to - and after their introduction. The late blight control strategy should be adapted accordingly. The Dutch P. infestans population was monitored during the growing seasons 2006 – 2008. P. infestans isolates were collected from blighted production fields and from bait fields in which R gene containing potato clones were grown without fungicide protection. A selection of the P. infestans isolates collected were characterized for virulence to a range of new R genes using a detached leaf bio-assay. Virulence for all single R genes tested was found. When we focus on R genes Rpi-blb1 and Rpi-blb2, no virulence was found in 2006. One Rpi-blb1 virulent isolate was found in 2007. Another 2007 isolate was found to be virulent to Rpi-blb2. Depending on the genetic background in which Rpi-blb1 was placed 13 or 21 isolates were virulent in 2008. Depending on the genetic background in which Rpi-blb2 was placed 4 or 11 isolates were virulent in 2008. One isolate was found to infect the stacked Rpi-blb1 and Rpi-blb2 resistance genes in a detached leaf assay. From these findings it is recommended that monitoring systems should be part of future potato late blight control strategies. The resulting information on the dynamics of virulence within the local P. infestans population can then be used to enhance the durability of newly introduced host resistance
Original languageEnglish
Title of host publicationProceedings of the Twelfth Euroblight Workshop, 3-6 May 2010, Arras, France
EditorsH.T.A.M. Schepers
Place of PublicationLelystad
PublisherApplied Plant Research
Pages45-49
Volume14
Publication statusPublished - 2010
EventTwelfth EuroBlight Workshop - Arras, France
Duration: 3 May 20106 May 2010

Workshop

WorkshopTwelfth EuroBlight Workshop
CountryFrance
CityArras
Period3/05/106/05/10

Fingerprint

Phytophthora infestans
virulence
monitoring
genes
durability
genetic background
breeding
assays
baits
leaves
fungicides
growing season
potatoes
clones
breeds
genome
pathogens

Cite this

Förch, M. G., van den Bosch, G. B. M., van Bekkum, P. J., Evenhuis, A., & Vossen, J. H. (2010). Monitoring the Dutch Phytophtora infestans population for virulence against new R-genes. In H. T. A. M. Schepers (Ed.), Proceedings of the Twelfth Euroblight Workshop, 3-6 May 2010, Arras, France (Vol. 14, pp. 45-49). Lelystad: Applied Plant Research.
Förch, M.G. ; van den Bosch, G.B.M. ; van Bekkum, P.J. ; Evenhuis, A. ; Vossen, J.H. / Monitoring the Dutch Phytophtora infestans population for virulence against new R-genes. Proceedings of the Twelfth Euroblight Workshop, 3-6 May 2010, Arras, France. editor / H.T.A.M. Schepers. Vol. 14 Lelystad : Applied Plant Research, 2010. pp. 45-49
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title = "Monitoring the Dutch Phytophtora infestans population for virulence against new R-genes",
abstract = "New possibilities offered by marker assisted breeding and GMO breeding have sparked renewed international efforts to breed for durable potato late blight resistance. Phytophthora infestans is however known for its adaptability, a trait confirmed by recent discoveries on the structure of the P. infestans genome. One of the possibilities to enhance the durability of newly introduced host resistance is to monitor the pathogen population for virulence to new R genes, prior to - and after their introduction. The late blight control strategy should be adapted accordingly. The Dutch P. infestans population was monitored during the growing seasons 2006 – 2008. P. infestans isolates were collected from blighted production fields and from bait fields in which R gene containing potato clones were grown without fungicide protection. A selection of the P. infestans isolates collected were characterized for virulence to a range of new R genes using a detached leaf bio-assay. Virulence for all single R genes tested was found. When we focus on R genes Rpi-blb1 and Rpi-blb2, no virulence was found in 2006. One Rpi-blb1 virulent isolate was found in 2007. Another 2007 isolate was found to be virulent to Rpi-blb2. Depending on the genetic background in which Rpi-blb1 was placed 13 or 21 isolates were virulent in 2008. Depending on the genetic background in which Rpi-blb2 was placed 4 or 11 isolates were virulent in 2008. One isolate was found to infect the stacked Rpi-blb1 and Rpi-blb2 resistance genes in a detached leaf assay. From these findings it is recommended that monitoring systems should be part of future potato late blight control strategies. The resulting information on the dynamics of virulence within the local P. infestans population can then be used to enhance the durability of newly introduced host resistance",
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year = "2010",
language = "English",
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Förch, MG, van den Bosch, GBM, van Bekkum, PJ, Evenhuis, A & Vossen, JH 2010, Monitoring the Dutch Phytophtora infestans population for virulence against new R-genes. in HTAM Schepers (ed.), Proceedings of the Twelfth Euroblight Workshop, 3-6 May 2010, Arras, France. vol. 14, Applied Plant Research, Lelystad, pp. 45-49, Twelfth EuroBlight Workshop, Arras, France, 3/05/10.

Monitoring the Dutch Phytophtora infestans population for virulence against new R-genes. / Förch, M.G.; van den Bosch, G.B.M.; van Bekkum, P.J.; Evenhuis, A.; Vossen, J.H.

Proceedings of the Twelfth Euroblight Workshop, 3-6 May 2010, Arras, France. ed. / H.T.A.M. Schepers. Vol. 14 Lelystad : Applied Plant Research, 2010. p. 45-49.

Research output: Chapter in Book/Report/Conference proceedingConference paperAcademic

TY - GEN

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AU - van Bekkum, P.J.

AU - Evenhuis, A.

AU - Vossen, J.H.

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N2 - New possibilities offered by marker assisted breeding and GMO breeding have sparked renewed international efforts to breed for durable potato late blight resistance. Phytophthora infestans is however known for its adaptability, a trait confirmed by recent discoveries on the structure of the P. infestans genome. One of the possibilities to enhance the durability of newly introduced host resistance is to monitor the pathogen population for virulence to new R genes, prior to - and after their introduction. The late blight control strategy should be adapted accordingly. The Dutch P. infestans population was monitored during the growing seasons 2006 – 2008. P. infestans isolates were collected from blighted production fields and from bait fields in which R gene containing potato clones were grown without fungicide protection. A selection of the P. infestans isolates collected were characterized for virulence to a range of new R genes using a detached leaf bio-assay. Virulence for all single R genes tested was found. When we focus on R genes Rpi-blb1 and Rpi-blb2, no virulence was found in 2006. One Rpi-blb1 virulent isolate was found in 2007. Another 2007 isolate was found to be virulent to Rpi-blb2. Depending on the genetic background in which Rpi-blb1 was placed 13 or 21 isolates were virulent in 2008. Depending on the genetic background in which Rpi-blb2 was placed 4 or 11 isolates were virulent in 2008. One isolate was found to infect the stacked Rpi-blb1 and Rpi-blb2 resistance genes in a detached leaf assay. From these findings it is recommended that monitoring systems should be part of future potato late blight control strategies. The resulting information on the dynamics of virulence within the local P. infestans population can then be used to enhance the durability of newly introduced host resistance

AB - New possibilities offered by marker assisted breeding and GMO breeding have sparked renewed international efforts to breed for durable potato late blight resistance. Phytophthora infestans is however known for its adaptability, a trait confirmed by recent discoveries on the structure of the P. infestans genome. One of the possibilities to enhance the durability of newly introduced host resistance is to monitor the pathogen population for virulence to new R genes, prior to - and after their introduction. The late blight control strategy should be adapted accordingly. The Dutch P. infestans population was monitored during the growing seasons 2006 – 2008. P. infestans isolates were collected from blighted production fields and from bait fields in which R gene containing potato clones were grown without fungicide protection. A selection of the P. infestans isolates collected were characterized for virulence to a range of new R genes using a detached leaf bio-assay. Virulence for all single R genes tested was found. When we focus on R genes Rpi-blb1 and Rpi-blb2, no virulence was found in 2006. One Rpi-blb1 virulent isolate was found in 2007. Another 2007 isolate was found to be virulent to Rpi-blb2. Depending on the genetic background in which Rpi-blb1 was placed 13 or 21 isolates were virulent in 2008. Depending on the genetic background in which Rpi-blb2 was placed 4 or 11 isolates were virulent in 2008. One isolate was found to infect the stacked Rpi-blb1 and Rpi-blb2 resistance genes in a detached leaf assay. From these findings it is recommended that monitoring systems should be part of future potato late blight control strategies. The resulting information on the dynamics of virulence within the local P. infestans population can then be used to enhance the durability of newly introduced host resistance

M3 - Conference paper

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EP - 49

BT - Proceedings of the Twelfth Euroblight Workshop, 3-6 May 2010, Arras, France

A2 - Schepers, H.T.A.M.

PB - Applied Plant Research

CY - Lelystad

ER -

Förch MG, van den Bosch GBM, van Bekkum PJ, Evenhuis A, Vossen JH. Monitoring the Dutch Phytophtora infestans population for virulence against new R-genes. In Schepers HTAM, editor, Proceedings of the Twelfth Euroblight Workshop, 3-6 May 2010, Arras, France. Vol. 14. Lelystad: Applied Plant Research. 2010. p. 45-49