Monitoring soil bacteria with community-level physiological profiles using Biolog™ ECO-plates in the Netherlands and Europe

Michiel Rutgers, Marja Wouterse, Sytske M. Drost, Anton M. Breure, Christian Mulder, Dorothy Stone, Rachel E. Creamer, Anne Winding, Jaap Bloem

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Soil samples were analyzed with community-level physiological profiles (CLPP) using Biolog™ ECO-plates in the Netherlands Soil Monitoring Network (NSMN; 704 samples) and in a European-wide transect (73 samples). The selection of sites was based on a representative sample of major soil texture types, land uses and climate zones. This method for determination of CLPP was specifically designed to be applied in large and long-term monitoring activities. It encompassed a solution for the inoculum-density dependence of colour formation patterns in ECO-plates, through stepwise dilution to extinction of the bacterial inoculum. The CLPP in Dutch and European soil samples appeared to be reproducible and sensitive to land use and/or soil texture. Although the method is selective, CLPP based parameters correlated well with other microbial parameters and soil characteristics. Consistent patterns in CLPP and soil habitat characteristics are emerging, as brought about by environmental disturbances, land management and soil texture. The applicability of CLPP analysis in monitoring systems is discussed.

LanguageEnglish
Pages23-35
JournalApplied Soil Ecology
Volume97
DOIs
Publication statusPublished - 2016

Fingerprint

soil bacteria
soil texture
Netherlands
Soil
Bacteria
bacterium
monitoring
soil sampling
land use
inoculum density
soil
land management
sampling
inoculum
extinction
environmental disturbance
density dependence
color
monitoring system
methodology

Keywords

  • Biolog
  • Indicator
  • Method
  • Microbial community
  • MicroResp
  • Soil

Cite this

Rutgers, Michiel ; Wouterse, Marja ; Drost, Sytske M. ; Breure, Anton M. ; Mulder, Christian ; Stone, Dorothy ; Creamer, Rachel E. ; Winding, Anne ; Bloem, Jaap. / Monitoring soil bacteria with community-level physiological profiles using Biolog™ ECO-plates in the Netherlands and Europe. In: Applied Soil Ecology. 2016 ; Vol. 97. pp. 23-35.
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Monitoring soil bacteria with community-level physiological profiles using Biolog™ ECO-plates in the Netherlands and Europe. / Rutgers, Michiel; Wouterse, Marja; Drost, Sytske M.; Breure, Anton M.; Mulder, Christian; Stone, Dorothy; Creamer, Rachel E.; Winding, Anne; Bloem, Jaap.

In: Applied Soil Ecology, Vol. 97, 2016, p. 23-35.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Drost, Sytske M.

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AU - Mulder, Christian

AU - Stone, Dorothy

AU - Creamer, Rachel E.

AU - Winding, Anne

AU - Bloem, Jaap

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