Molecularly assessed shifts of Bifidobacterium spp. and less diverse microbial communities are characteristic of 5-year-old allergic children

J. Stsepetova, E. Sepp, K. Julge, E.E. Vaughan, M. Mikelsaar, W.M. de Vos

Research output: Contribution to journalArticleAcademicpeer-review

47 Citations (Scopus)

Abstract

The composition of intestinal microbiota and the Bifidobacterium group community in 20 allergic and 20 nonallergic 5-year-old children was visualized by PCR-denaturing gradient gel electrophoresis (DGGE). The number of dominant bands in the DGGE profiles was smaller in allergic children than in nonallergic children (P
Original languageEnglish
Pages (from-to)260-269
JournalFEMS Immunology and Medical Microbiology
Volume51
Issue number2
DOIs
Publication statusPublished - 2007

Keywords

  • 16s ribosomal-rna
  • gradient gel-electrophoresis
  • species-specific primers
  • lactic-acid bacteria
  • intestinal microflora
  • gastrointestinal-tract
  • estonian children
  • fecal microflora
  • targeted probes
  • colonic-mucosa

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