Molecular methods for the analysis of gut microbiota

P. Namsolleck; R. Thiel; P. Lawson; K. Holmstrom; M. Rajilic-Stojanovic; E.E. Vaughan; L. Rigottier-Gois; M.D. Collins; W.M. de Vos; M. Blaut

doi:10.1080/08910600410032367

Molecular methods for the analysis of gut microbiota

P. Namsolleck, R. Thiel, P. Lawson, K. Holmstrom, M. Rajilic-Stojanovic, E.E. Vaughan, L. Rigottier-Gois, M.D. Collins, W.M. de Vos, M. Blaut

Research output: Contribution to journal › Article › Academic › peer-review

30 Citations (Scopus)

Abstract

This review focuses on methodological approaches used to study the composition of human faecal microbiota. Gene sequencing is the most accurate tool for revealing the phylogenetic relationships between bacteria. The main application of fluorescence in situ hybridization (FISH) in both microscopy and flow cytometry is to enumerate faecal bacteria. While flow cytometry is a very fast method, FISH microscopy still has a considerably lower detection limit.

Original language	English
Pages (from-to)	71-85
Journal	Microbial Ecology in Health and Disease
Volume	16
Issue number	2-3
DOIs	https://doi.org/10.1080/08910600410032367
Publication status	Published - 2004

Keywords

DGGE
Dot blot
FISH
Flow cytometry
Gut ecology
In situ gene expression
Micro-array
Sequencing
TGGE

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10.1080/08910600410032367

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title = "Molecular methods for the analysis of gut microbiota",

abstract = "This review focuses on methodological approaches used to study the composition of human faecal microbiota. Gene sequencing is the most accurate tool for revealing the phylogenetic relationships between bacteria. The main application of fluorescence in situ hybridization (FISH) in both microscopy and flow cytometry is to enumerate faecal bacteria. While flow cytometry is a very fast method, FISH microscopy still has a considerably lower detection limit.",

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AU - Namsolleck, P.

AU - Thiel, R.

AU - Lawson, P.

AU - Holmstrom, K.

AU - Rajilic-Stojanovic, M.

AU - Vaughan, E.E.

AU - Rigottier-Gois, L.

AU - Collins, M.D.

AU - de Vos, W.M.

AU - Blaut, M.

PY - 2004

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AB - This review focuses on methodological approaches used to study the composition of human faecal microbiota. Gene sequencing is the most accurate tool for revealing the phylogenetic relationships between bacteria. The main application of fluorescence in situ hybridization (FISH) in both microscopy and flow cytometry is to enumerate faecal bacteria. While flow cytometry is a very fast method, FISH microscopy still has a considerably lower detection limit.

KW - DGGE

KW - Dot blot

KW - FISH

KW - Flow cytometry

KW - Gut ecology

KW - In situ gene expression

KW - Micro-array

KW - Sequencing

KW - TGGE

U2 - 10.1080/08910600410032367

DO - 10.1080/08910600410032367

M3 - Article

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EP - 85

JO - Microbial Ecology in Health and Disease

JF - Microbial Ecology in Health and Disease

IS - 2-3

ER -

Molecular methods for the analysis of gut microbiota

Abstract

Keywords

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