Molecular Identification of an Isolate of Peanut Mottle Virus (PeMoV) in Iran

N. Beikzadeh, A. Hassani-Mehraban, D. Peters

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Peanut plants showing mottling, yellow and necrotic spots on leaves were collected from peanut fields in Golestan province. Electron microscopic studies revealed the presence of flexuous filamentous particles ca. 700 nm in length, which was suggestive of a potyvirus infection. Healthy Nicotiana benthamiana plants mechanically inoculated with sap from infected peanut plants showed mottling, downward leaf curling, and wrinkling of the leaves. The virus was transmitted by Myzus persicae in a non-persistent manner to healthy N. benthamiana, on which symptoms were observed two weeks later. RT-PCR using an Oligo-dT and a NIb primer set resulted in a fragment of about 1093 bp, which comprised the complete coat protein (CP) gene and 3´-non-coding region. Analysis of its CP nucleotide and amino acid sequence revealed 98-99% similarity and 95-99% identity to those of Peanut mottle virus (PeMoV) isolated from other countries, respectively. The molecular data confirmed serological, vector transmission, and electron microscopic findings on the incidence of PeMoV in Iran. Additionally, sequence and phylogenetic analyses of the CP revealed clustering of Iranian PeMoV isolate with Asian/Australian isolates.

LanguageEnglish
Article number21
Pages765-776
JournalJournal of Agricultural Science and Technology (JAST)
Volume17
Issue number3
Publication statusPublished - 2015

Fingerprint

Peanut mottle virus
Iran
coat proteins
peanuts
Nicotiana benthamiana
Viruses
Capsid Proteins
electrons
leaf curling
Potyvirus
Myzus persicae
sap
signs and symptoms (plants)
leaves
amino acid sequences
nucleotides
reverse transcriptase polymerase chain reaction
Electrons
incidence
viruses

Keywords

  • Arachis hypogea
  • Phylogenetic analysis
  • Potyviridae

Cite this

Beikzadeh, N. ; Hassani-Mehraban, A. ; Peters, D. / Molecular Identification of an Isolate of Peanut Mottle Virus (PeMoV) in Iran. In: Journal of Agricultural Science and Technology (JAST). 2015 ; Vol. 17, No. 3. pp. 765-776.
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abstract = "Peanut plants showing mottling, yellow and necrotic spots on leaves were collected from peanut fields in Golestan province. Electron microscopic studies revealed the presence of flexuous filamentous particles ca. 700 nm in length, which was suggestive of a potyvirus infection. Healthy Nicotiana benthamiana plants mechanically inoculated with sap from infected peanut plants showed mottling, downward leaf curling, and wrinkling of the leaves. The virus was transmitted by Myzus persicae in a non-persistent manner to healthy N. benthamiana, on which symptoms were observed two weeks later. RT-PCR using an Oligo-dT and a NIb primer set resulted in a fragment of about 1093 bp, which comprised the complete coat protein (CP) gene and 3´-non-coding region. Analysis of its CP nucleotide and amino acid sequence revealed 98-99{\%} similarity and 95-99{\%} identity to those of Peanut mottle virus (PeMoV) isolated from other countries, respectively. The molecular data confirmed serological, vector transmission, and electron microscopic findings on the incidence of PeMoV in Iran. Additionally, sequence and phylogenetic analyses of the CP revealed clustering of Iranian PeMoV isolate with Asian/Australian isolates.",
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Beikzadeh, N, Hassani-Mehraban, A & Peters, D 2015, 'Molecular Identification of an Isolate of Peanut Mottle Virus (PeMoV) in Iran', Journal of Agricultural Science and Technology (JAST), vol. 17, no. 3, 21, pp. 765-776.

Molecular Identification of an Isolate of Peanut Mottle Virus (PeMoV) in Iran. / Beikzadeh, N.; Hassani-Mehraban, A.; Peters, D.

In: Journal of Agricultural Science and Technology (JAST), Vol. 17, No. 3, 21, 2015, p. 765-776.

Research output: Contribution to journalArticleAcademicpeer-review

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N2 - Peanut plants showing mottling, yellow and necrotic spots on leaves were collected from peanut fields in Golestan province. Electron microscopic studies revealed the presence of flexuous filamentous particles ca. 700 nm in length, which was suggestive of a potyvirus infection. Healthy Nicotiana benthamiana plants mechanically inoculated with sap from infected peanut plants showed mottling, downward leaf curling, and wrinkling of the leaves. The virus was transmitted by Myzus persicae in a non-persistent manner to healthy N. benthamiana, on which symptoms were observed two weeks later. RT-PCR using an Oligo-dT and a NIb primer set resulted in a fragment of about 1093 bp, which comprised the complete coat protein (CP) gene and 3´-non-coding region. Analysis of its CP nucleotide and amino acid sequence revealed 98-99% similarity and 95-99% identity to those of Peanut mottle virus (PeMoV) isolated from other countries, respectively. The molecular data confirmed serological, vector transmission, and electron microscopic findings on the incidence of PeMoV in Iran. Additionally, sequence and phylogenetic analyses of the CP revealed clustering of Iranian PeMoV isolate with Asian/Australian isolates.

AB - Peanut plants showing mottling, yellow and necrotic spots on leaves were collected from peanut fields in Golestan province. Electron microscopic studies revealed the presence of flexuous filamentous particles ca. 700 nm in length, which was suggestive of a potyvirus infection. Healthy Nicotiana benthamiana plants mechanically inoculated with sap from infected peanut plants showed mottling, downward leaf curling, and wrinkling of the leaves. The virus was transmitted by Myzus persicae in a non-persistent manner to healthy N. benthamiana, on which symptoms were observed two weeks later. RT-PCR using an Oligo-dT and a NIb primer set resulted in a fragment of about 1093 bp, which comprised the complete coat protein (CP) gene and 3´-non-coding region. Analysis of its CP nucleotide and amino acid sequence revealed 98-99% similarity and 95-99% identity to those of Peanut mottle virus (PeMoV) isolated from other countries, respectively. The molecular data confirmed serological, vector transmission, and electron microscopic findings on the incidence of PeMoV in Iran. Additionally, sequence and phylogenetic analyses of the CP revealed clustering of Iranian PeMoV isolate with Asian/Australian isolates.

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