Molecular hazard identification of non-O157 Shiga toxin-producing Escherichia coli (STEC)

E. Franz, Angela H.A.M. van Hoek, Mark Wuite, F.J. van der Wal, A.G. de Boer, E.L. Bouw, Henk J.M. Aarts

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Abstract

The complexity regarding Shiga toxin-producing Escherichia coli (STEC) in food safety enforcement as well as clinical care primarily relates to the current inability of an accurate risk assessment of individual strains due to the large variety in serotype and genetic content associated with (severe) disease. In order to classify the clinical and/or epidemic potential of a STEC isolate at an early stage it is crucial to identify virulence characteristics of putative pathogens from genomic information, which is referred to as ‘predictive hazard identification’. This study aimed at identifying associations between virulence factors, phylogenetic groups, isolation sources and seropathotypes. Most non-O157 STEC in the Netherlands belong to phylogroup B1 and are characterized by the presence of ehxA, iha and stx2, but absence of eae. The large variability in the number of virulence factors present among serogroups and seropathotypes demonstrated that this was merely indicative for the virulence potential. While all the virulence gene associations have been worked out, it appeared that there is no specific pattern that would unambiguously enable hazard identification for an STEC strain. However, the strong correlations between virulence factors indicate that these arrays are not a random collection but are rather specific sets. Especially the presence of eae was strongly correlated to the presence of many of the other virulence genes, including all non-LEE encoded effectors. Different stx-subtypes were associated with different virulence profiles. The factors ehxA and ureC were significantly associated with HUS-associated strains (HAS) and not correlated to the presence of eae. This indicates their candidacy as important pathogenicity markers next to eae and stx2a.
Original languageEnglish
Article numbere0120353
JournalPLoS ONE
Volume10
Issue number3
DOIs
Publication statusPublished - 2015

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hazard identification
Shiga Toxin
Shiga-Toxigenic Escherichia coli
Shiga toxin-producing Escherichia coli
Escherichia coli
Virulence
Hazards
Virulence Factors
virulence
Genes
Food safety
Pathogens
Risk assessment
serotypes
Food Safety
Netherlands
risk assessment
food safety
pathogenicity
genes

Cite this

Franz, E., van Hoek, A. H. A. M., Wuite, M., van der Wal, F. J., de Boer, A. G., Bouw, E. L., & Aarts, H. J. M. (2015). Molecular hazard identification of non-O157 Shiga toxin-producing Escherichia coli (STEC). PLoS ONE, 10(3), [e0120353]. https://doi.org/10.1371/journal.pone.0120353
Franz, E. ; van Hoek, Angela H.A.M. ; Wuite, Mark ; van der Wal, F.J. ; de Boer, A.G. ; Bouw, E.L. ; Aarts, Henk J.M. / Molecular hazard identification of non-O157 Shiga toxin-producing Escherichia coli (STEC). In: PLoS ONE. 2015 ; Vol. 10, No. 3.
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abstract = "The complexity regarding Shiga toxin-producing Escherichia coli (STEC) in food safety enforcement as well as clinical care primarily relates to the current inability of an accurate risk assessment of individual strains due to the large variety in serotype and genetic content associated with (severe) disease. In order to classify the clinical and/or epidemic potential of a STEC isolate at an early stage it is crucial to identify virulence characteristics of putative pathogens from genomic information, which is referred to as ‘predictive hazard identification’. This study aimed at identifying associations between virulence factors, phylogenetic groups, isolation sources and seropathotypes. Most non-O157 STEC in the Netherlands belong to phylogroup B1 and are characterized by the presence of ehxA, iha and stx2, but absence of eae. The large variability in the number of virulence factors present among serogroups and seropathotypes demonstrated that this was merely indicative for the virulence potential. While all the virulence gene associations have been worked out, it appeared that there is no specific pattern that would unambiguously enable hazard identification for an STEC strain. However, the strong correlations between virulence factors indicate that these arrays are not a random collection but are rather specific sets. Especially the presence of eae was strongly correlated to the presence of many of the other virulence genes, including all non-LEE encoded effectors. Different stx-subtypes were associated with different virulence profiles. The factors ehxA and ureC were significantly associated with HUS-associated strains (HAS) and not correlated to the presence of eae. This indicates their candidacy as important pathogenicity markers next to eae and stx2a.",
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Franz, E, van Hoek, AHAM, Wuite, M, van der Wal, FJ, de Boer, AG, Bouw, EL & Aarts, HJM 2015, 'Molecular hazard identification of non-O157 Shiga toxin-producing Escherichia coli (STEC)' PLoS ONE, vol. 10, no. 3, e0120353. https://doi.org/10.1371/journal.pone.0120353

Molecular hazard identification of non-O157 Shiga toxin-producing Escherichia coli (STEC). / Franz, E.; van Hoek, Angela H.A.M.; Wuite, Mark; van der Wal, F.J.; de Boer, A.G.; Bouw, E.L.; Aarts, Henk J.M.

In: PLoS ONE, Vol. 10, No. 3, e0120353, 2015.

Research output: Contribution to journalArticleAcademicpeer-review

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AB - The complexity regarding Shiga toxin-producing Escherichia coli (STEC) in food safety enforcement as well as clinical care primarily relates to the current inability of an accurate risk assessment of individual strains due to the large variety in serotype and genetic content associated with (severe) disease. In order to classify the clinical and/or epidemic potential of a STEC isolate at an early stage it is crucial to identify virulence characteristics of putative pathogens from genomic information, which is referred to as ‘predictive hazard identification’. This study aimed at identifying associations between virulence factors, phylogenetic groups, isolation sources and seropathotypes. Most non-O157 STEC in the Netherlands belong to phylogroup B1 and are characterized by the presence of ehxA, iha and stx2, but absence of eae. The large variability in the number of virulence factors present among serogroups and seropathotypes demonstrated that this was merely indicative for the virulence potential. While all the virulence gene associations have been worked out, it appeared that there is no specific pattern that would unambiguously enable hazard identification for an STEC strain. However, the strong correlations between virulence factors indicate that these arrays are not a random collection but are rather specific sets. Especially the presence of eae was strongly correlated to the presence of many of the other virulence genes, including all non-LEE encoded effectors. Different stx-subtypes were associated with different virulence profiles. The factors ehxA and ureC were significantly associated with HUS-associated strains (HAS) and not correlated to the presence of eae. This indicates their candidacy as important pathogenicity markers next to eae and stx2a.

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