Molecular genetic analysis of the pathogenicity of the potato cyst nematode Globodera rostochiensis

L. Qin

Research output: Thesisinternal PhD, WUAcademic

Abstract

<p>A new strategy to identify pathogenicity factors from the potato cyst nematode <em>Globodera rostochiensis</em> is developed. cDNA-AFLP technology and <em>in situ</em> hybridization allowed us to efficiently select putative pathogenicity factors among thousands of expressed genes. As a result, an unprecedented number of putative pathogenicity factors from this obligatory plant parasite were cloned.</p><p>A powerful bioinformatics tool named GenEST to link expression data generated from cDNA-AFLP directly to cDNA sequence data was developed. This computer program would be very useful for functional genomics studies in other systems as well.</p><p>A RanBPM (Ran-Binding Protein in Microtubule organization)-like gene family was identified. The proteins encoded by these genes are probably secreted by the nematode into plant cells and might manipulate the host cell development by changing the dynamic instability of microtubules.</p><p>A promoter region from the potato cyst nematode was shown to be functional in a distantly related nematode species <em>Caenorhabditis elegans</em> . This promoter could be a valuable tool to drive gene expression in transgenic plant-parasitic nematode species to assess the relative importance of putative pathogenicity factors.</p><p>A paper based from one chapter of this thesis will be submitted to Science. In view of their press embargo policy, I will not discussed the detail of this particular finding here.</p>
LanguageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Bakker, Jaap, Promotor
  • Helder, Hans, Promotor
  • Smant, Geert, Co-promotor
Award date5 Oct 2001
Place of PublicationS.l.
Publisher
Print ISBNs9789058084521
Publication statusPublished - 2001

Fingerprint

Globodera rostochiensis
cyst nematodes
molecular genetics
genetic techniques and protocols
pathogenicity
potatoes
plant parasitic nematodes
microtubules
amplified fragment length polymorphism
promoter regions
genes
Caenorhabditis elegans
bioinformatics
in situ hybridization
binding proteins
transgenic plants
Nematoda
cells
genomics
parasites

Keywords

  • plant parasitic nematodes
  • globodera rostochiensis
  • pathogenicity
  • complementary dna
  • polymorphism
  • gene expression
  • molecular genetics
  • bioinformatics

Cite this

@phdthesis{a8433444d35b402685a34964aec2bf8e,
title = "Molecular genetic analysis of the pathogenicity of the potato cyst nematode Globodera rostochiensis",
abstract = "A new strategy to identify pathogenicity factors from the potato cyst nematode Globodera rostochiensis is developed. cDNA-AFLP technology and in situ hybridization allowed us to efficiently select putative pathogenicity factors among thousands of expressed genes. As a result, an unprecedented number of putative pathogenicity factors from this obligatory plant parasite were cloned.A powerful bioinformatics tool named GenEST to link expression data generated from cDNA-AFLP directly to cDNA sequence data was developed. This computer program would be very useful for functional genomics studies in other systems as well.A RanBPM (Ran-Binding Protein in Microtubule organization)-like gene family was identified. The proteins encoded by these genes are probably secreted by the nematode into plant cells and might manipulate the host cell development by changing the dynamic instability of microtubules.A promoter region from the potato cyst nematode was shown to be functional in a distantly related nematode species Caenorhabditis elegans . This promoter could be a valuable tool to drive gene expression in transgenic plant-parasitic nematode species to assess the relative importance of putative pathogenicity factors.A paper based from one chapter of this thesis will be submitted to Science. In view of their press embargo policy, I will not discussed the detail of this particular finding here.",
keywords = "plantenparasitaire nematoden, globodera rostochiensis, complementair dna, polymorfisme, genexpressie, pathogeniteit, moleculaire genetica, bio-informatica, plant parasitic nematodes, globodera rostochiensis, pathogenicity, complementary dna, polymorphism, gene expression, molecular genetics, bioinformatics",
author = "L. Qin",
note = "WU thesis 3044 Met lit. opg. - Met samenvatting in het Engels Proefschrift Wageningen Universiteit",
year = "2001",
language = "English",
isbn = "9789058084521",
publisher = "S.n.",
school = "Wageningen University",

}

Qin, L 2001, 'Molecular genetic analysis of the pathogenicity of the potato cyst nematode Globodera rostochiensis', Doctor of Philosophy, Wageningen University, S.l..

Molecular genetic analysis of the pathogenicity of the potato cyst nematode Globodera rostochiensis. / Qin, L.

S.l. : S.n., 2001.

Research output: Thesisinternal PhD, WUAcademic

TY - THES

T1 - Molecular genetic analysis of the pathogenicity of the potato cyst nematode Globodera rostochiensis

AU - Qin, L.

N1 - WU thesis 3044 Met lit. opg. - Met samenvatting in het Engels Proefschrift Wageningen Universiteit

PY - 2001

Y1 - 2001

N2 - A new strategy to identify pathogenicity factors from the potato cyst nematode Globodera rostochiensis is developed. cDNA-AFLP technology and in situ hybridization allowed us to efficiently select putative pathogenicity factors among thousands of expressed genes. As a result, an unprecedented number of putative pathogenicity factors from this obligatory plant parasite were cloned.A powerful bioinformatics tool named GenEST to link expression data generated from cDNA-AFLP directly to cDNA sequence data was developed. This computer program would be very useful for functional genomics studies in other systems as well.A RanBPM (Ran-Binding Protein in Microtubule organization)-like gene family was identified. The proteins encoded by these genes are probably secreted by the nematode into plant cells and might manipulate the host cell development by changing the dynamic instability of microtubules.A promoter region from the potato cyst nematode was shown to be functional in a distantly related nematode species Caenorhabditis elegans . This promoter could be a valuable tool to drive gene expression in transgenic plant-parasitic nematode species to assess the relative importance of putative pathogenicity factors.A paper based from one chapter of this thesis will be submitted to Science. In view of their press embargo policy, I will not discussed the detail of this particular finding here.

AB - A new strategy to identify pathogenicity factors from the potato cyst nematode Globodera rostochiensis is developed. cDNA-AFLP technology and in situ hybridization allowed us to efficiently select putative pathogenicity factors among thousands of expressed genes. As a result, an unprecedented number of putative pathogenicity factors from this obligatory plant parasite were cloned.A powerful bioinformatics tool named GenEST to link expression data generated from cDNA-AFLP directly to cDNA sequence data was developed. This computer program would be very useful for functional genomics studies in other systems as well.A RanBPM (Ran-Binding Protein in Microtubule organization)-like gene family was identified. The proteins encoded by these genes are probably secreted by the nematode into plant cells and might manipulate the host cell development by changing the dynamic instability of microtubules.A promoter region from the potato cyst nematode was shown to be functional in a distantly related nematode species Caenorhabditis elegans . This promoter could be a valuable tool to drive gene expression in transgenic plant-parasitic nematode species to assess the relative importance of putative pathogenicity factors.A paper based from one chapter of this thesis will be submitted to Science. In view of their press embargo policy, I will not discussed the detail of this particular finding here.

KW - plantenparasitaire nematoden

KW - globodera rostochiensis

KW - complementair dna

KW - polymorfisme

KW - genexpressie

KW - pathogeniteit

KW - moleculaire genetica

KW - bio-informatica

KW - plant parasitic nematodes

KW - globodera rostochiensis

KW - pathogenicity

KW - complementary dna

KW - polymorphism

KW - gene expression

KW - molecular genetics

KW - bioinformatics

M3 - internal PhD, WU

SN - 9789058084521

PB - S.n.

CY - S.l.

ER -