Molecular dynamics simulations reveal that AEDANS is an inert fluorescent probe for the study of membrane proteins

W.L. Vos, M. Schor, A. Baumgaertner, D.P. Tieleman, M.A. Hemminga

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Computer simulations were carried out of a number of AEDANS-labeled single cysteine mutants of a small reference membrane protein, M13 major coat protein, covering 60% of its primary sequence. M13 major coat protein is a single membrane-spanning, a-helical membrane protein with a relatively large water-exposed region in the N-terminus. In 10-ns molecular dynamics simulations, we analyze the behavior of the AEDANS label and the native tryptophan, which were used as acceptor and donor in previous FRET experiments. The results indicate that AEDANS is a relatively inert environmental probe that can move unhindered through the lipid membrane when attached to a membrane protein
Original languageEnglish
Pages (from-to)229-239
JournalEuropean Biophysics Journal
Volume39
Issue number2
DOIs
Publication statusPublished - 2010

Keywords

  • major coat protein
  • transmembrane alpha-helix
  • energy-transfer
  • fret
  • orientation
  • conformation
  • spectroscopy
  • model
  • association
  • bilayers

Fingerprint Dive into the research topics of 'Molecular dynamics simulations reveal that AEDANS is an inert fluorescent probe for the study of membrane proteins'. Together they form a unique fingerprint.

Cite this