Molecular determination and genetic modification of flower colour in Lilium spp

Nur Fatihah Hasan Nudin

Research output: Thesisinternal PhD, WU

Abstract

Lily (genus Lilium, family Liliaceae) is one of the most important ornamental crops for the production of flower bulbs and cut flowers. Conventional breeding might be inefficient for introducing variation in flower colours in L. longiflorum due to hybrid sterility and deficiency of genetic resources in related, crossable species. An alternative is provided by the technology of genetic modification, but lily is among the monocot species that is recalcitrant to genetic transformation by A. tumefaciens and thus exhibits a low transformation efficiency (TE). There is a need to enhance TE in lily. Because lily transformation is proven to be difficult and time consuming, we developed and optimized a simple agroinfiltration method in order to monitor transient gene expression of anthocyanin structural genes and regulatory genes in lily tepals. In addition, we determined molecular mechanisms regulating flower colour in lilies by an expression analysis of anthocyanin biosynthesis-related genes during multiple flower developmental stages. As a result, DFR was found to be the main bottleneck for not having colour in L. longiflorum cv. ‘Lincoln’ and the Oriental hybrid cv. ‘Rialto’.  A follow up of this research should focus on a careful selection and characterization of a functional and suitable DFR gene copy from other lilies or another crop in order to produce novel flower colour in both cultivars.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Visser, Richard, Promotor
  • Krens, Frans, Co-promotor
Award date30 Nov 2018
Place of PublicationWageningen
Publisher
Print ISBNs9789463435208
DOIs
Publication statusPublished - 2018

Fingerprint

Lilium
genetic engineering
flowers
color
anthocyanins
agroinfiltration
Liliaceae
structural genes
cut flowers
genetic transformation
regulator genes
Liliopsida
genetic resources
ornamental plants
bulbs
crop production
genes
developmental stages
biosynthesis
gene expression

Cite this

Fatihah Hasan Nudin, Nur. / Molecular determination and genetic modification of flower colour in Lilium spp. Wageningen : Wageningen University, 2018. 170 p.
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title = "Molecular determination and genetic modification of flower colour in Lilium spp",
abstract = "Lily (genus Lilium, family Liliaceae) is one of the most important ornamental crops for the production of flower bulbs and cut flowers. Conventional breeding might be inefficient for introducing variation in flower colours in L. longiflorum due to hybrid sterility and deficiency of genetic resources in related, crossable species. An alternative is provided by the technology of genetic modification, but lily is among the monocot species that is recalcitrant to genetic transformation by A. tumefaciens and thus exhibits a low transformation efficiency (TE). There is a need to enhance TE in lily. Because lily transformation is proven to be difficult and time consuming, we developed and optimized a simple agroinfiltration method in order to monitor transient gene expression of anthocyanin structural genes and regulatory genes in lily tepals. In addition, we determined molecular mechanisms regulating flower colour in lilies by an expression analysis of anthocyanin biosynthesis-related genes during multiple flower developmental stages. As a result, DFR was found to be the main bottleneck for not having colour in L. longiflorum cv. ‘Lincoln’ and the Oriental hybrid cv. ‘Rialto’.  A follow up of this research should focus on a careful selection and characterization of a functional and suitable DFR gene copy from other lilies or another crop in order to produce novel flower colour in both cultivars.",
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Fatihah Hasan Nudin, N 2018, 'Molecular determination and genetic modification of flower colour in Lilium spp', Doctor of Philosophy, Wageningen University, Wageningen. https://doi.org/10.18174/461249

Molecular determination and genetic modification of flower colour in Lilium spp. / Fatihah Hasan Nudin, Nur.

Wageningen : Wageningen University, 2018. 170 p.

Research output: Thesisinternal PhD, WU

TY - THES

T1 - Molecular determination and genetic modification of flower colour in Lilium spp

AU - Fatihah Hasan Nudin, Nur

N1 - WU thesis 7100 Includes bibliographical references. - With summary in English

PY - 2018

Y1 - 2018

N2 - Lily (genus Lilium, family Liliaceae) is one of the most important ornamental crops for the production of flower bulbs and cut flowers. Conventional breeding might be inefficient for introducing variation in flower colours in L. longiflorum due to hybrid sterility and deficiency of genetic resources in related, crossable species. An alternative is provided by the technology of genetic modification, but lily is among the monocot species that is recalcitrant to genetic transformation by A. tumefaciens and thus exhibits a low transformation efficiency (TE). There is a need to enhance TE in lily. Because lily transformation is proven to be difficult and time consuming, we developed and optimized a simple agroinfiltration method in order to monitor transient gene expression of anthocyanin structural genes and regulatory genes in lily tepals. In addition, we determined molecular mechanisms regulating flower colour in lilies by an expression analysis of anthocyanin biosynthesis-related genes during multiple flower developmental stages. As a result, DFR was found to be the main bottleneck for not having colour in L. longiflorum cv. ‘Lincoln’ and the Oriental hybrid cv. ‘Rialto’.  A follow up of this research should focus on a careful selection and characterization of a functional and suitable DFR gene copy from other lilies or another crop in order to produce novel flower colour in both cultivars.

AB - Lily (genus Lilium, family Liliaceae) is one of the most important ornamental crops for the production of flower bulbs and cut flowers. Conventional breeding might be inefficient for introducing variation in flower colours in L. longiflorum due to hybrid sterility and deficiency of genetic resources in related, crossable species. An alternative is provided by the technology of genetic modification, but lily is among the monocot species that is recalcitrant to genetic transformation by A. tumefaciens and thus exhibits a low transformation efficiency (TE). There is a need to enhance TE in lily. Because lily transformation is proven to be difficult and time consuming, we developed and optimized a simple agroinfiltration method in order to monitor transient gene expression of anthocyanin structural genes and regulatory genes in lily tepals. In addition, we determined molecular mechanisms regulating flower colour in lilies by an expression analysis of anthocyanin biosynthesis-related genes during multiple flower developmental stages. As a result, DFR was found to be the main bottleneck for not having colour in L. longiflorum cv. ‘Lincoln’ and the Oriental hybrid cv. ‘Rialto’.  A follow up of this research should focus on a careful selection and characterization of a functional and suitable DFR gene copy from other lilies or another crop in order to produce novel flower colour in both cultivars.

U2 - 10.18174/461249

DO - 10.18174/461249

M3 - internal PhD, WU

SN - 9789463435208

PB - Wageningen University

CY - Wageningen

ER -