Molecular cloning and expression of a Toll receptor in the giant tiger shrimp, Penaeus monodon

J.A.J. Arts, F. Cornelissen, T. Cijsouw, G.J. Hermsen, H.F.J. Savelkoul, R.J.M. Stet

Research output: Contribution to journalArticleAcademicpeer-review

99 Citations (Scopus)

Abstract

Invertebrates rely completely for their protection against pathogens on the innate immune system. This non-self-recognition is activated by microbial cell wall components with unique conserved molecular patterns. Pathogen-associated molecular patterns (PAMPs) are recognised by pattern recognition receptors (PRRs). Toll and its mammalian homologs Toll-like receptors are cell-surface receptors acting as PRRs and involved in the signalling pathway implicated in their immune response. Here we describe a novel partial Toll receptor gene cloned from a gill library of the giant tiger shrimp, Penaeus monodon, using primers based on the highly conserved Toll/IL-1R (TIR) domain. The deduced amino acid sequence of the P. monodon Toll (PmToll) shows 59% similarity to a Toll-related protein of Apis mellifera. Analysis of the LRRs of shrimp Toll contained no obvious PAMP-binding insertions. Phylogenetic analysis with the insect Toll family shows clustering with Toll1 and Toll5 gene products, and it is less related to Toll3 and Toll4. Furthermore, RT-qPCR shows that PmToll is constitutively expressed in gut, gill and hepatopancreas. Challenge with white spot syndrome virus (WSSV) shows equal levels of expression in these organs. A role in the defence mechanism is discussed. In conclusion, shrimp possess at least one Toll receptor that might be involved in immune defence.
Original languageEnglish
Pages (from-to)504-513
JournalFish and Shellfish Immunology
Volume23
Issue number3
DOIs
Publication statusPublished - 2007

Keywords

  • peptidoglycan recognition protein
  • drosophila toll
  • immune-responses
  • activation
  • pathway
  • sequence
  • database
  • crustaceans
  • evolution
  • bacteria

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