Molecular characterizations of human and animal group a Rotaviruses in the Netherlands

R. van der Heide, M.P.G. Koopmans, N. Shekary, D.J. Houwers, Y. van Duynhoven, W.H.M. van der Poel

    Research output: Contribution to journalArticleAcademicpeer-review

    34 Citations (Scopus)

    Abstract

    To gain more insight into interspecies transmission of rotavirus group A, human and animal fecal samples were collected between 1997 and 2001 in The Netherlands. A total of 110 human stool samples were successfully P and G genotyped by reverse transcriptase PCR. All strains belonged to the main human rotavirus genotypes G1 to G4, G9, [P4], [P6], [P8], and [P9]. [P8]G1 was predominant, and 5.5% belonged to the G9 genotype. Eleven percent of all P[8] genotypes could be genotyped only by a recently published modified primer. Rotavirus-positive fecal samples from 28 calf herds were genotyped by DNA sequencing. Genotypes G6 and G10 predominated; G6 and G10 were detected in 22 (78.6%) and 16 (57.1%) of the rotavirus-positive calf herds, respectively. In 12 (42.9%) calf herds, we found mixed infections. Genotype G8 was not found. Genotype G6 bovine rotaviruses were divided into three clusters: UK-like, VMRI-29-like, and Hun4-like. DNA sequencing of a part of the VP7 gene was shown to be useful as a quick determination of uncommon or novel strains of which the genotyping cannot be done by genotyping PCR. Of equine strains, both VP4 and VP7 genes could be used for genotyping: two [P12]G3 and four [P12]G14 equine rotaviruses were determined. We did not find indications for rotavirus interspecies transmissions, although the recently published human G6-Hun4 is genetically related to our G6 bovine isolates. All bovine, porcine, and equine rotaviruses were within genotypes previously reported for these animal species.
    Original languageEnglish
    Pages (from-to)669-675
    JournalJournal of Clinical Microbiology
    Volume43
    Issue number2
    DOIs
    Publication statusPublished - 2005

    Fingerprint

    Rotavirus
    Netherlands
    Genotype
    Horses
    DNA Sequence Analysis
    Reverse Transcriptase Polymerase Chain Reaction
    Coinfection
    Genes
    Swine
    Polymerase Chain Reaction

    Keywords

    • polymerase chain-reaction
    • sentinel general practices
    • bovine rotaviruses
    • acute gastroenteritis
    • genetic-variability
    • sequence-analysis
    • p-serotypes
    • strains
    • pcr
    • vp4

    Cite this

    van der Heide, R. ; Koopmans, M.P.G. ; Shekary, N. ; Houwers, D.J. ; van Duynhoven, Y. ; van der Poel, W.H.M. / Molecular characterizations of human and animal group a Rotaviruses in the Netherlands. In: Journal of Clinical Microbiology. 2005 ; Vol. 43, No. 2. pp. 669-675.
    @article{e70401a7856e400580553e718cae151d,
    title = "Molecular characterizations of human and animal group a Rotaviruses in the Netherlands",
    abstract = "To gain more insight into interspecies transmission of rotavirus group A, human and animal fecal samples were collected between 1997 and 2001 in The Netherlands. A total of 110 human stool samples were successfully P and G genotyped by reverse transcriptase PCR. All strains belonged to the main human rotavirus genotypes G1 to G4, G9, [P4], [P6], [P8], and [P9]. [P8]G1 was predominant, and 5.5{\%} belonged to the G9 genotype. Eleven percent of all P[8] genotypes could be genotyped only by a recently published modified primer. Rotavirus-positive fecal samples from 28 calf herds were genotyped by DNA sequencing. Genotypes G6 and G10 predominated; G6 and G10 were detected in 22 (78.6{\%}) and 16 (57.1{\%}) of the rotavirus-positive calf herds, respectively. In 12 (42.9{\%}) calf herds, we found mixed infections. Genotype G8 was not found. Genotype G6 bovine rotaviruses were divided into three clusters: UK-like, VMRI-29-like, and Hun4-like. DNA sequencing of a part of the VP7 gene was shown to be useful as a quick determination of uncommon or novel strains of which the genotyping cannot be done by genotyping PCR. Of equine strains, both VP4 and VP7 genes could be used for genotyping: two [P12]G3 and four [P12]G14 equine rotaviruses were determined. We did not find indications for rotavirus interspecies transmissions, although the recently published human G6-Hun4 is genetically related to our G6 bovine isolates. All bovine, porcine, and equine rotaviruses were within genotypes previously reported for these animal species.",
    keywords = "polymerase chain-reaction, sentinel general practices, bovine rotaviruses, acute gastroenteritis, genetic-variability, sequence-analysis, p-serotypes, strains, pcr, vp4",
    author = "{van der Heide}, R. and M.P.G. Koopmans and N. Shekary and D.J. Houwers and {van Duynhoven}, Y. and {van der Poel}, W.H.M.",
    note = "ISI:000227045600022",
    year = "2005",
    doi = "10.1128/JCM.43.2.669-675.2005",
    language = "English",
    volume = "43",
    pages = "669--675",
    journal = "Journal of Clinical Microbiology",
    issn = "0095-1137",
    publisher = "American Society for Microbiology",
    number = "2",

    }

    Molecular characterizations of human and animal group a Rotaviruses in the Netherlands. / van der Heide, R.; Koopmans, M.P.G.; Shekary, N.; Houwers, D.J.; van Duynhoven, Y.; van der Poel, W.H.M.

    In: Journal of Clinical Microbiology, Vol. 43, No. 2, 2005, p. 669-675.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Molecular characterizations of human and animal group a Rotaviruses in the Netherlands

    AU - van der Heide, R.

    AU - Koopmans, M.P.G.

    AU - Shekary, N.

    AU - Houwers, D.J.

    AU - van Duynhoven, Y.

    AU - van der Poel, W.H.M.

    N1 - ISI:000227045600022

    PY - 2005

    Y1 - 2005

    N2 - To gain more insight into interspecies transmission of rotavirus group A, human and animal fecal samples were collected between 1997 and 2001 in The Netherlands. A total of 110 human stool samples were successfully P and G genotyped by reverse transcriptase PCR. All strains belonged to the main human rotavirus genotypes G1 to G4, G9, [P4], [P6], [P8], and [P9]. [P8]G1 was predominant, and 5.5% belonged to the G9 genotype. Eleven percent of all P[8] genotypes could be genotyped only by a recently published modified primer. Rotavirus-positive fecal samples from 28 calf herds were genotyped by DNA sequencing. Genotypes G6 and G10 predominated; G6 and G10 were detected in 22 (78.6%) and 16 (57.1%) of the rotavirus-positive calf herds, respectively. In 12 (42.9%) calf herds, we found mixed infections. Genotype G8 was not found. Genotype G6 bovine rotaviruses were divided into three clusters: UK-like, VMRI-29-like, and Hun4-like. DNA sequencing of a part of the VP7 gene was shown to be useful as a quick determination of uncommon or novel strains of which the genotyping cannot be done by genotyping PCR. Of equine strains, both VP4 and VP7 genes could be used for genotyping: two [P12]G3 and four [P12]G14 equine rotaviruses were determined. We did not find indications for rotavirus interspecies transmissions, although the recently published human G6-Hun4 is genetically related to our G6 bovine isolates. All bovine, porcine, and equine rotaviruses were within genotypes previously reported for these animal species.

    AB - To gain more insight into interspecies transmission of rotavirus group A, human and animal fecal samples were collected between 1997 and 2001 in The Netherlands. A total of 110 human stool samples were successfully P and G genotyped by reverse transcriptase PCR. All strains belonged to the main human rotavirus genotypes G1 to G4, G9, [P4], [P6], [P8], and [P9]. [P8]G1 was predominant, and 5.5% belonged to the G9 genotype. Eleven percent of all P[8] genotypes could be genotyped only by a recently published modified primer. Rotavirus-positive fecal samples from 28 calf herds were genotyped by DNA sequencing. Genotypes G6 and G10 predominated; G6 and G10 were detected in 22 (78.6%) and 16 (57.1%) of the rotavirus-positive calf herds, respectively. In 12 (42.9%) calf herds, we found mixed infections. Genotype G8 was not found. Genotype G6 bovine rotaviruses were divided into three clusters: UK-like, VMRI-29-like, and Hun4-like. DNA sequencing of a part of the VP7 gene was shown to be useful as a quick determination of uncommon or novel strains of which the genotyping cannot be done by genotyping PCR. Of equine strains, both VP4 and VP7 genes could be used for genotyping: two [P12]G3 and four [P12]G14 equine rotaviruses were determined. We did not find indications for rotavirus interspecies transmissions, although the recently published human G6-Hun4 is genetically related to our G6 bovine isolates. All bovine, porcine, and equine rotaviruses were within genotypes previously reported for these animal species.

    KW - polymerase chain-reaction

    KW - sentinel general practices

    KW - bovine rotaviruses

    KW - acute gastroenteritis

    KW - genetic-variability

    KW - sequence-analysis

    KW - p-serotypes

    KW - strains

    KW - pcr

    KW - vp4

    U2 - 10.1128/JCM.43.2.669-675.2005

    DO - 10.1128/JCM.43.2.669-675.2005

    M3 - Article

    VL - 43

    SP - 669

    EP - 675

    JO - Journal of Clinical Microbiology

    JF - Journal of Clinical Microbiology

    SN - 0095-1137

    IS - 2

    ER -