Molecular characterizations of human and animal group a Rotaviruses in the Netherlands

R. van der Heide, M.P.G. Koopmans, N. Shekary, D.J. Houwers, Y. van Duynhoven, W.H.M. van der Poel

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    35 Citations (Scopus)


    To gain more insight into interspecies transmission of rotavirus group A, human and animal fecal samples were collected between 1997 and 2001 in The Netherlands. A total of 110 human stool samples were successfully P and G genotyped by reverse transcriptase PCR. All strains belonged to the main human rotavirus genotypes G1 to G4, G9, [P4], [P6], [P8], and [P9]. [P8]G1 was predominant, and 5.5% belonged to the G9 genotype. Eleven percent of all P[8] genotypes could be genotyped only by a recently published modified primer. Rotavirus-positive fecal samples from 28 calf herds were genotyped by DNA sequencing. Genotypes G6 and G10 predominated; G6 and G10 were detected in 22 (78.6%) and 16 (57.1%) of the rotavirus-positive calf herds, respectively. In 12 (42.9%) calf herds, we found mixed infections. Genotype G8 was not found. Genotype G6 bovine rotaviruses were divided into three clusters: UK-like, VMRI-29-like, and Hun4-like. DNA sequencing of a part of the VP7 gene was shown to be useful as a quick determination of uncommon or novel strains of which the genotyping cannot be done by genotyping PCR. Of equine strains, both VP4 and VP7 genes could be used for genotyping: two [P12]G3 and four [P12]G14 equine rotaviruses were determined. We did not find indications for rotavirus interspecies transmissions, although the recently published human G6-Hun4 is genetically related to our G6 bovine isolates. All bovine, porcine, and equine rotaviruses were within genotypes previously reported for these animal species.
    Original languageEnglish
    Pages (from-to)669-675
    JournalJournal of Clinical Microbiology
    Issue number2
    Publication statusPublished - 2005


    • polymerase chain-reaction
    • sentinel general practices
    • bovine rotaviruses
    • acute gastroenteritis
    • genetic-variability
    • sequence-analysis
    • p-serotypes
    • strains
    • pcr
    • vp4

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