Molecular characterization of nucleus-associated virulence targets of effector GpRbp-1 from Globodera pallida

Research output: Thesisinternal PhD, WU

Abstract

Soil-borne cyst nematodes are obligatory sedentary parasites that induce extraordinary modifications in the roots of their hosts, thereby causing severe losses to cultivation of major crops. The alterations of plant morphology and physiology are required for the establishment of specialised permanent feeding sites, which solely support the sedentary life style of the nematodes. Nematodes secrete effectors which target host proteins to mediate this drastic cellular reprogramming of the plant. GpRbp-1 from the largely expanded family of SPRYSEC effectors in the potato cyst nematode Globodera pallida acts as an avirulence factor in the presence of potato immune receptor Gpa2. Nevertheless, its virulence function in plants in the absence of the receptor remains undescribed. In this thesis, we determined and characterised the plant targets of GpRbp-1 to elucidate its function in virulence. We used a combination of protein-protein interaction methods, in vivo assays, and transcriptomics to demonstrate that GpRbp-1 targets the SUMO E3-ligase SIZ1, the Ubiquitin E3-ligase UPL3, and a Gpa2 recognition co-factor the Ran-activating GTPase RanGAP. Our findings suggest that GpRbp-1 may function by modulating plant immunity mediated by salicylic acid and the Ran cycle to promote susceptibility to nematodes. Finally, we propose mechanistic models for the activity of GpRbp-1 and discuss them in the wider context of plant-pathogen interactions. 

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Smant, Geert, Promotor
  • Goverse, Aska, Co-promotor
Award date24 Oct 2019
Place of PublicationWageningen
Publisher
Print ISBNs9789463950602
DOIs
Publication statusPublished - 2019

Fingerprint

Globodera pallida
virulence
ubiquitin-protein ligase
cyst nematodes
Nematoda
potatoes
plant morphology
mechanistic models
plant physiology
guanosinetriphosphatase
protein-protein interactions
transcriptomics
salicylic acid
plant pathogens
lifestyle
immunity
parasites
receptors
assays
crops

Cite this

@phdthesis{c6c35ec084c344989a8e9717f2b92fe2,
title = "Molecular characterization of nucleus-associated virulence targets of effector GpRbp-1 from Globodera pallida",
abstract = "Soil-borne cyst nematodes are obligatory sedentary parasites that induce extraordinary modifications in the roots of their hosts, thereby causing severe losses to cultivation of major crops. The alterations of plant morphology and physiology are required for the establishment of specialised permanent feeding sites, which solely support the sedentary life style of the nematodes. Nematodes secrete effectors which target host proteins to mediate this drastic cellular reprogramming of the plant. GpRbp-1 from the largely expanded family of SPRYSEC effectors in the potato cyst nematode Globodera pallida acts as an avirulence factor in the presence of potato immune receptor Gpa2. Nevertheless, its virulence function in plants in the absence of the receptor remains undescribed. In this thesis, we determined and characterised the plant targets of GpRbp-1 to elucidate its function in virulence. We used a combination of protein-protein interaction methods, in vivo assays, and transcriptomics to demonstrate that GpRbp-1 targets the SUMO E3-ligase SIZ1, the Ubiquitin E3-ligase UPL3, and a Gpa2 recognition co-factor the Ran-activating GTPase RanGAP. Our findings suggest that GpRbp-1 may function by modulating plant immunity mediated by salicylic acid and the Ran cycle to promote susceptibility to nematodes. Finally, we propose mechanistic models for the activity of GpRbp-1 and discuss them in the wider context of plant-pathogen interactions. ",
author = "{Diaz Granados Muñoz}, Amalia",
note = "WU thesis 7352 Includes bibliographical references. - With summaries in English, Dutch and Spanish",
year = "2019",
doi = "10.18174/497314",
language = "English",
isbn = "9789463950602",
publisher = "Wageningen University",
school = "Wageningen University",

}

Molecular characterization of nucleus-associated virulence targets of effector GpRbp-1 from Globodera pallida. / Diaz Granados Muñoz, Amalia.

Wageningen : Wageningen University, 2019. 191 p.

Research output: Thesisinternal PhD, WU

TY - THES

T1 - Molecular characterization of nucleus-associated virulence targets of effector GpRbp-1 from Globodera pallida

AU - Diaz Granados Muñoz, Amalia

N1 - WU thesis 7352 Includes bibliographical references. - With summaries in English, Dutch and Spanish

PY - 2019

Y1 - 2019

N2 - Soil-borne cyst nematodes are obligatory sedentary parasites that induce extraordinary modifications in the roots of their hosts, thereby causing severe losses to cultivation of major crops. The alterations of plant morphology and physiology are required for the establishment of specialised permanent feeding sites, which solely support the sedentary life style of the nematodes. Nematodes secrete effectors which target host proteins to mediate this drastic cellular reprogramming of the plant. GpRbp-1 from the largely expanded family of SPRYSEC effectors in the potato cyst nematode Globodera pallida acts as an avirulence factor in the presence of potato immune receptor Gpa2. Nevertheless, its virulence function in plants in the absence of the receptor remains undescribed. In this thesis, we determined and characterised the plant targets of GpRbp-1 to elucidate its function in virulence. We used a combination of protein-protein interaction methods, in vivo assays, and transcriptomics to demonstrate that GpRbp-1 targets the SUMO E3-ligase SIZ1, the Ubiquitin E3-ligase UPL3, and a Gpa2 recognition co-factor the Ran-activating GTPase RanGAP. Our findings suggest that GpRbp-1 may function by modulating plant immunity mediated by salicylic acid and the Ran cycle to promote susceptibility to nematodes. Finally, we propose mechanistic models for the activity of GpRbp-1 and discuss them in the wider context of plant-pathogen interactions. 

AB - Soil-borne cyst nematodes are obligatory sedentary parasites that induce extraordinary modifications in the roots of their hosts, thereby causing severe losses to cultivation of major crops. The alterations of plant morphology and physiology are required for the establishment of specialised permanent feeding sites, which solely support the sedentary life style of the nematodes. Nematodes secrete effectors which target host proteins to mediate this drastic cellular reprogramming of the plant. GpRbp-1 from the largely expanded family of SPRYSEC effectors in the potato cyst nematode Globodera pallida acts as an avirulence factor in the presence of potato immune receptor Gpa2. Nevertheless, its virulence function in plants in the absence of the receptor remains undescribed. In this thesis, we determined and characterised the plant targets of GpRbp-1 to elucidate its function in virulence. We used a combination of protein-protein interaction methods, in vivo assays, and transcriptomics to demonstrate that GpRbp-1 targets the SUMO E3-ligase SIZ1, the Ubiquitin E3-ligase UPL3, and a Gpa2 recognition co-factor the Ran-activating GTPase RanGAP. Our findings suggest that GpRbp-1 may function by modulating plant immunity mediated by salicylic acid and the Ran cycle to promote susceptibility to nematodes. Finally, we propose mechanistic models for the activity of GpRbp-1 and discuss them in the wider context of plant-pathogen interactions. 

U2 - 10.18174/497314

DO - 10.18174/497314

M3 - internal PhD, WU

SN - 9789463950602

PB - Wageningen University

CY - Wageningen

ER -