TY - JOUR
T1 - Molecular characterisation of uterine endometrial proteins during early stages of pregnancy in pigs by maldi tof/tof
AU - Pierzchała, Dorota
AU - Liput, Kamila
AU - Korwin-Kossakowska, Agnieszka
AU - Ogłuszka, Magdalena
AU - Poławska, Ewa
AU - Nawrocka, Agata
AU - Urbański, Paweł
AU - Ciepłoch, Aleksandra
AU - Juszczuk-Kubiak, Edyta
AU - Lepczyński, Adam
AU - Ślaska, Brygida
AU - Kowal, Krzysztof
AU - Te Pas, Marinus F.W.
AU - Śmiech, Magdalena
AU - Leszczyński, Paweł
AU - Taniguchi, Hiroaki
AU - Fraser, Leyland
AU - Sobiech, Przemysław
AU - Sachajko, Mateusz
AU - Herudzinska, Magdalena
AU - Pareek, Chandra S.
AU - Pierzchała, Mariusz
PY - 2021/6/23
Y1 - 2021/6/23
N2 - The molecular mechanism underlying embryonic implantation is vital to understand the correct communications between endometrium and developing conceptus during early stages of pregnancy. This study’s objective was to determine molecular changes in the uterine endometrial proteome during the preimplantation and peri-implantation between 9 days (9D), 12 days (12D), and 16 days (16D) of pregnant Polish Large White (PLW) gilts. 2DE-MALDI-TOF/TOF and Clue-GO™ approaches were employed to analyse the biological networks and molecular changes in porcine endometrial proteome during maternal recognition of pregnancy. A total of sixteen differentially expressed proteins (DEPs) were identified using 2-DE gels and MALDI-TOF/TOF mass spectrometry. Comparison between 9D and 12D of pregnancy identified APOA1, CAPZB, LDHB, CCT5, ANXA4, CFB, TTR upregulated DEPs, and ANXA5, SMS downregulated DEPs. Comparison between 9D and 16D of pregnancy identified HP, APOA1, ACTB, CCT5, ANXA4, CFB upregulated DEPs and ANXA5, SMS, LDHB, ACTR3, HP, ENO3, OAT downregulated DEPs. However, a comparison between 12D and 16D of pregnancy identified HP, ACTB upregulated DEPs, and CRYM, ANXA4, ANXA5, CAPZB, LDHB, ACTR3, CCT5, ENO3, OAT, TTR down-regulated DEPs. Outcomes of this study revealed key proteins and their interactions with metabolic pathways involved in the recognition and establishment of early pregnancy in PLW gilts
AB - The molecular mechanism underlying embryonic implantation is vital to understand the correct communications between endometrium and developing conceptus during early stages of pregnancy. This study’s objective was to determine molecular changes in the uterine endometrial proteome during the preimplantation and peri-implantation between 9 days (9D), 12 days (12D), and 16 days (16D) of pregnant Polish Large White (PLW) gilts. 2DE-MALDI-TOF/TOF and Clue-GO™ approaches were employed to analyse the biological networks and molecular changes in porcine endometrial proteome during maternal recognition of pregnancy. A total of sixteen differentially expressed proteins (DEPs) were identified using 2-DE gels and MALDI-TOF/TOF mass spectrometry. Comparison between 9D and 12D of pregnancy identified APOA1, CAPZB, LDHB, CCT5, ANXA4, CFB, TTR upregulated DEPs, and ANXA5, SMS downregulated DEPs. Comparison between 9D and 16D of pregnancy identified HP, APOA1, ACTB, CCT5, ANXA4, CFB upregulated DEPs and ANXA5, SMS, LDHB, ACTR3, HP, ENO3, OAT downregulated DEPs. However, a comparison between 12D and 16D of pregnancy identified HP, ACTB upregulated DEPs, and CRYM, ANXA4, ANXA5, CAPZB, LDHB, ACTR3, CCT5, ENO3, OAT, TTR down-regulated DEPs. Outcomes of this study revealed key proteins and their interactions with metabolic pathways involved in the recognition and establishment of early pregnancy in PLW gilts
KW - Biomarkers
KW - Differentially expressed proteins
KW - Endometrium
KW - Implantation
KW - MALDI TOF/TOF
KW - Pigs
KW - Polish Large White
KW - Pregnancy
KW - Proteome
U2 - 10.3390/ijms22136720
DO - 10.3390/ijms22136720
M3 - Article
AN - SCOPUS:85108325406
SN - 1661-6596
VL - 22
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 13
M1 - 6720
ER -