Molecular characterisation of uterine endometrial proteins during early stages of pregnancy in pigs by maldi tof/tof

Dorota Pierzchała, Kamila Liput, Agnieszka Korwin-Kossakowska, Magdalena Ogłuszka, Ewa Poławska, Agata Nawrocka, Paweł Urbański, Aleksandra Ciepłoch, Edyta Juszczuk-Kubiak, Adam Lepczyński, Brygida Ślaska, Krzysztof Kowal, Marinus F.W. Te Pas, Magdalena Śmiech, Paweł Leszczyński, Hiroaki Taniguchi, Leyland Fraser, Przemysław Sobiech, Mateusz Sachajko, Magdalena HerudzinskaChandra S. Pareek, Mariusz Pierzchała*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

The molecular mechanism underlying embryonic implantation is vital to understand the correct communications between endometrium and developing conceptus during early stages of pregnancy. This study’s objective was to determine molecular changes in the uterine endometrial proteome during the preimplantation and peri-implantation between 9 days (9D), 12 days (12D), and 16 days (16D) of pregnant Polish Large White (PLW) gilts. 2DE-MALDI-TOF/TOF and Clue-GO™ approaches were employed to analyse the biological networks and molecular changes in porcine endometrial proteome during maternal recognition of pregnancy. A total of sixteen differentially expressed proteins (DEPs) were identified using 2-DE gels and MALDI-TOF/TOF mass spectrometry. Comparison between 9D and 12D of pregnancy identified APOA1, CAPZB, LDHB, CCT5, ANXA4, CFB, TTR upregulated DEPs, and ANXA5, SMS downregulated DEPs. Comparison between 9D and 16D of pregnancy identified HP, APOA1, ACTB, CCT5, ANXA4, CFB upregulated DEPs and ANXA5, SMS, LDHB, ACTR3, HP, ENO3, OAT downregulated DEPs. However, a comparison between 12D and 16D of pregnancy identified HP, ACTB upregulated DEPs, and CRYM, ANXA4, ANXA5, CAPZB, LDHB, ACTR3, CCT5, ENO3, OAT, TTR down-regulated DEPs. Outcomes of this study revealed key proteins and their interactions with metabolic pathways involved in the recognition and establishment of early pregnancy in PLW gilts

Original languageEnglish
Article number6720
JournalInternational Journal of Molecular Sciences
Volume22
Issue number13
DOIs
Publication statusPublished - 23 Jun 2021

Keywords

  • Biomarkers
  • Differentially expressed proteins
  • Endometrium
  • Implantation
  • MALDI TOF/TOF
  • Pigs
  • Polish Large White
  • Pregnancy
  • Proteome

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