Modulation of the chicken immune cell function by dietary polyunsaturated fatty acids

J.W.C. Sijben

Research output: Thesisinternal PhD, WUAcademic

Abstract

Polyunsaturated fatty acids (PUFA) possess a wide range of biological properties, including immunomodulation. The amount, type, and ratio of dietary PUFA determine the types of fatty acids that are incorporated into immune cell membranes. Consequently, the physiological properties of immune cells and their potential to produce communication molecules, such as eicosanoids, can be modulated. This thesis aims to describe the (interaction) effects of n-3 and n-6 PUFA after challenge with antigens of various nature, and thereby to identify PUFA requirements for optimal immune responses in chickens. Antibody responses to model antigens known to induce T helper-1 and T helper-2 type responses in mice were increased or decreased by n-6, and increased or not affected by n-3 PUFA, depending on the antigen, and levels of other PUFA. Cutaneous hypersensitivity, an index for in vivo T cell reactivity, was increased by n-3 and decreased by n-6 PUFA or not affected. In vitro T cell reactivity was increased in chickens fed n-3 PUFA enriched diet. Evidence that vitamin E interacts with PUFA effects on immune cell function, particularly at high PUFA levels, was not found. Cytokine mRNA levels early after LPS challenge were increased by long-chain n-3 PUFA or not affected by dietary PUFA. The present thesis indicates that dietary PUFA have the potential to modulate chicken immune cell function, but that most effects are the contrary of what is usually found in mammals. The differences are possibly due to differences in chickens and mammals with regard to the metabolism and effector functions of PGE 2 , and the effects of dietary n-3 on cytokine production. It is hypothesized that inclusion of 1-2 % of n-3 and inclusion of no more than 3-4 % of n-6 in the diet, is optimal for Ab responsiveness, enhances T cell reactivity, and possibly improves chicken's disease

LanguageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Kemp, Bas, Promotor
  • Parmentier, Henk, Co-promotor
  • Schrama, Johan, Co-promotor
Award date18 Jan 2003
Place of Publication[S.l.]
Publisher
Print ISBNs905808552X
Publication statusPublished - 2002

Fingerprint

polyunsaturated fatty acids
chickens
cells
omega-3 fatty acids
T-lymphocytes
omega-6 fatty acids
antigens
cytokines
mammals
poultry diseases
immunomodulation
eicosanoids
diet
hypersensitivity
animal communication
vitamin E
cell membranes
immune response
fatty acids
antibodies

Keywords

  • hens
  • fowls
  • polyenoic fatty acids
  • hen feeding
  • feed additives
  • immune response
  • immune system
  • immunity
  • nutrient improvement
  • nutrition physiology
  • animal health

Cite this

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title = "Modulation of the chicken immune cell function by dietary polyunsaturated fatty acids",
abstract = "Polyunsaturated fatty acids (PUFA) possess a wide range of biological properties, including immunomodulation. The amount, type, and ratio of dietary PUFA determine the types of fatty acids that are incorporated into immune cell membranes. Consequently, the physiological properties of immune cells and their potential to produce communication molecules, such as eicosanoids, can be modulated. This thesis aims to describe the (interaction) effects of n-3 and n-6 PUFA after challenge with antigens of various nature, and thereby to identify PUFA requirements for optimal immune responses in chickens. Antibody responses to model antigens known to induce T helper-1 and T helper-2 type responses in mice were increased or decreased by n-6, and increased or not affected by n-3 PUFA, depending on the antigen, and levels of other PUFA. Cutaneous hypersensitivity, an index for in vivo T cell reactivity, was increased by n-3 and decreased by n-6 PUFA or not affected. In vitro T cell reactivity was increased in chickens fed n-3 PUFA enriched diet. Evidence that vitamin E interacts with PUFA effects on immune cell function, particularly at high PUFA levels, was not found. Cytokine mRNA levels early after LPS challenge were increased by long-chain n-3 PUFA or not affected by dietary PUFA. The present thesis indicates that dietary PUFA have the potential to modulate chicken immune cell function, but that most effects are the contrary of what is usually found in mammals. The differences are possibly due to differences in chickens and mammals with regard to the metabolism and effector functions of PGE 2 , and the effects of dietary n-3 on cytokine production. It is hypothesized that inclusion of 1-2 {\%} of n-3 and inclusion of no more than 3-4 {\%} of n-6 in the diet, is optimal for Ab responsiveness, enhances T cell reactivity, and possibly improves chicken's disease",
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author = "J.W.C. Sijben",
note = "WU thesis no. 3134",
year = "2002",
language = "English",
isbn = "905808552X",
publisher = "S.n.",
school = "Wageningen University",

}

Sijben, JWC 2002, 'Modulation of the chicken immune cell function by dietary polyunsaturated fatty acids', Doctor of Philosophy, Wageningen University, [S.l.].

Modulation of the chicken immune cell function by dietary polyunsaturated fatty acids. / Sijben, J.W.C.

[S.l.] : S.n., 2002. 155 p.

Research output: Thesisinternal PhD, WUAcademic

TY - THES

T1 - Modulation of the chicken immune cell function by dietary polyunsaturated fatty acids

AU - Sijben, J.W.C.

N1 - WU thesis no. 3134

PY - 2002

Y1 - 2002

N2 - Polyunsaturated fatty acids (PUFA) possess a wide range of biological properties, including immunomodulation. The amount, type, and ratio of dietary PUFA determine the types of fatty acids that are incorporated into immune cell membranes. Consequently, the physiological properties of immune cells and their potential to produce communication molecules, such as eicosanoids, can be modulated. This thesis aims to describe the (interaction) effects of n-3 and n-6 PUFA after challenge with antigens of various nature, and thereby to identify PUFA requirements for optimal immune responses in chickens. Antibody responses to model antigens known to induce T helper-1 and T helper-2 type responses in mice were increased or decreased by n-6, and increased or not affected by n-3 PUFA, depending on the antigen, and levels of other PUFA. Cutaneous hypersensitivity, an index for in vivo T cell reactivity, was increased by n-3 and decreased by n-6 PUFA or not affected. In vitro T cell reactivity was increased in chickens fed n-3 PUFA enriched diet. Evidence that vitamin E interacts with PUFA effects on immune cell function, particularly at high PUFA levels, was not found. Cytokine mRNA levels early after LPS challenge were increased by long-chain n-3 PUFA or not affected by dietary PUFA. The present thesis indicates that dietary PUFA have the potential to modulate chicken immune cell function, but that most effects are the contrary of what is usually found in mammals. The differences are possibly due to differences in chickens and mammals with regard to the metabolism and effector functions of PGE 2 , and the effects of dietary n-3 on cytokine production. It is hypothesized that inclusion of 1-2 % of n-3 and inclusion of no more than 3-4 % of n-6 in the diet, is optimal for Ab responsiveness, enhances T cell reactivity, and possibly improves chicken's disease

AB - Polyunsaturated fatty acids (PUFA) possess a wide range of biological properties, including immunomodulation. The amount, type, and ratio of dietary PUFA determine the types of fatty acids that are incorporated into immune cell membranes. Consequently, the physiological properties of immune cells and their potential to produce communication molecules, such as eicosanoids, can be modulated. This thesis aims to describe the (interaction) effects of n-3 and n-6 PUFA after challenge with antigens of various nature, and thereby to identify PUFA requirements for optimal immune responses in chickens. Antibody responses to model antigens known to induce T helper-1 and T helper-2 type responses in mice were increased or decreased by n-6, and increased or not affected by n-3 PUFA, depending on the antigen, and levels of other PUFA. Cutaneous hypersensitivity, an index for in vivo T cell reactivity, was increased by n-3 and decreased by n-6 PUFA or not affected. In vitro T cell reactivity was increased in chickens fed n-3 PUFA enriched diet. Evidence that vitamin E interacts with PUFA effects on immune cell function, particularly at high PUFA levels, was not found. Cytokine mRNA levels early after LPS challenge were increased by long-chain n-3 PUFA or not affected by dietary PUFA. The present thesis indicates that dietary PUFA have the potential to modulate chicken immune cell function, but that most effects are the contrary of what is usually found in mammals. The differences are possibly due to differences in chickens and mammals with regard to the metabolism and effector functions of PGE 2 , and the effects of dietary n-3 on cytokine production. It is hypothesized that inclusion of 1-2 % of n-3 and inclusion of no more than 3-4 % of n-6 in the diet, is optimal for Ab responsiveness, enhances T cell reactivity, and possibly improves chicken's disease

KW - hennen

KW - kippen

KW - meervoudig onverzadigde vetzuren

KW - hennenvoeding

KW - voedertoevoegingen

KW - immuniteitsreactie

KW - immuunsysteem

KW - immuniteit

KW - voedingsstoffenverbetering

KW - voedingsfysiologie

KW - diergezondheid

KW - hens

KW - fowls

KW - polyenoic fatty acids

KW - hen feeding

KW - feed additives

KW - immune response

KW - immune system

KW - immunity

KW - nutrient improvement

KW - nutrition physiology

KW - animal health

M3 - internal PhD, WU

SN - 905808552X

PB - S.n.

CY - [S.l.]

ER -