Modification of wheat straw lignin by solid state fermentation with white-rot fungi

M.J. Dinis, R.M.F. Bezerra, F. Nunes, A.A. Dias, C. Guedes, L.M.M. Ferreira, J.W. Cone, G.S.M. Marques, A.R.N. Barros, M.A.M. Rodrigues

Research output: Contribution to journalArticleAcademicpeer-review

129 Citations (Scopus)

Abstract

The potential of crude enzyme extracts, obtained from solid state cultivation of four white-rot fungi (Trametes versicolor, Bjerkandera adusta, Ganoderma applanatum and Phlebia rufa), was exploited to modify wheat straw cell wall. At different fermentation times, manganese-dependent peroxidase (MnP), lignin peroxidase (LiP), laccase, carboxymethylcellulase (CMCase), avicelase, xylanase and feruloyl esterase activities were screened and the content of lignin as well as hydroxycinnamic acids in fermented straw were determined. All fungi secreted feruloyl esterase while LiP was only detected in crude extracts from B. adusta. Since no significant differences (P > 0.05) were observed in remaining lignin content of fermented straw, LiP activity was not a limiting factor of enzymatic lignin removal process. The levels of esterified hydroxycinnamic acids degradation were considerably higher than previous reports with lignocellulosic biomass. The data show that P. rufa, may be considered for more specific studies as higher ferulic and p-coumaric acids degradation was observed for earlier incubation times
Original languageEnglish
Pages (from-to)4829-4835
JournalBioresource Technology
Volume100
Issue number20
DOIs
Publication statusPublished - 2009

Keywords

  • plant-cell wall
  • feruloyl esterases
  • phenolic-compounds
  • manganese peroxidase
  • enzymes
  • hydrolysis
  • degradation
  • cellulose
  • release
  • laccase

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