Modification of potato cell wall pectin by the introduction of rhamnogalacturonan lyase and β-galactosidase transgenes and their side effects

Jie Hong Huang, Anne Kortstee, Dianka C.T. Dees, Luisa M. Trindade, Henk A. Schols, Harry Gruppen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Genes encoding pectic enzymes were introduced to wild-type potato Karnico. Cell wall materials were extracted from Karnico and transgenic lines expressing β-galactosidase (β-Gal-14 mutant) or rhamnogalacturonan lyase (RGL-18 mutant). After sequential extraction, β-Gal-14 hot buffer-soluble solids (HBSS) of pectin contained 54% less galactose than Karnico HBSS, representing shorter galactan side chains. The individual pectin populations of β-Gal-14 HBSS showed different modifications extended to the two sub-populations as obtained by ion-exchange chromatography. Compared to wild-type, RGL-18 HBSS contained 27% more galacturonic acid and 55% less Gal on fresh potato weight basis, which was due to the removal of galactan-rich rhamnogalacturonan I (RG-I) segments. All pectin populations of RGL-18 showed consistently low levels of RG-I segments. Transgenic modification showed side effects on the methyl-esterification and acetyl substitution of RGL-18 HBSS (DM = 53, DA = 21), but not of the β-Gal-14 HBSS in comparison to wild-type (DM = 29, DA = 54).

Original languageEnglish
Pages (from-to)9-16
JournalCarbohydrate Polymers
Volume144
DOIs
Publication statusPublished - 2016

Fingerprint

Galactosidases
Lyases
Buffers
Cells
Galactans
Gene encoding
Esterification
Chromatography
Galactose
pectin
Ion exchange
Substitution reactions
Enzymes
Acids

Keywords

  • Galactan side chains
  • Plant cell wall polysaccharides
  • Potato mutant
  • Potato pectin
  • Solanum tuberosum
  • Starch removal

Cite this

@article{fe25bceb30ed463da1a836561e56ff4d,
title = "Modification of potato cell wall pectin by the introduction of rhamnogalacturonan lyase and β-galactosidase transgenes and their side effects",
abstract = "Genes encoding pectic enzymes were introduced to wild-type potato Karnico. Cell wall materials were extracted from Karnico and transgenic lines expressing β-galactosidase (β-Gal-14 mutant) or rhamnogalacturonan lyase (RGL-18 mutant). After sequential extraction, β-Gal-14 hot buffer-soluble solids (HBSS) of pectin contained 54{\%} less galactose than Karnico HBSS, representing shorter galactan side chains. The individual pectin populations of β-Gal-14 HBSS showed different modifications extended to the two sub-populations as obtained by ion-exchange chromatography. Compared to wild-type, RGL-18 HBSS contained 27{\%} more galacturonic acid and 55{\%} less Gal on fresh potato weight basis, which was due to the removal of galactan-rich rhamnogalacturonan I (RG-I) segments. All pectin populations of RGL-18 showed consistently low levels of RG-I segments. Transgenic modification showed side effects on the methyl-esterification and acetyl substitution of RGL-18 HBSS (DM = 53, DA = 21), but not of the β-Gal-14 HBSS in comparison to wild-type (DM = 29, DA = 54).",
keywords = "Galactan side chains, Plant cell wall polysaccharides, Potato mutant, Potato pectin, Solanum tuberosum, Starch removal",
author = "Huang, {Jie Hong} and Anne Kortstee and Dees, {Dianka C.T.} and Trindade, {Luisa M.} and Schols, {Henk A.} and Harry Gruppen",
year = "2016",
doi = "10.1016/j.carbpol.2016.02.037",
language = "English",
volume = "144",
pages = "9--16",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
publisher = "Elsevier",

}

Modification of potato cell wall pectin by the introduction of rhamnogalacturonan lyase and β-galactosidase transgenes and their side effects. / Huang, Jie Hong; Kortstee, Anne; Dees, Dianka C.T.; Trindade, Luisa M.; Schols, Henk A.; Gruppen, Harry.

In: Carbohydrate Polymers, Vol. 144, 2016, p. 9-16.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Modification of potato cell wall pectin by the introduction of rhamnogalacturonan lyase and β-galactosidase transgenes and their side effects

AU - Huang, Jie Hong

AU - Kortstee, Anne

AU - Dees, Dianka C.T.

AU - Trindade, Luisa M.

AU - Schols, Henk A.

AU - Gruppen, Harry

PY - 2016

Y1 - 2016

N2 - Genes encoding pectic enzymes were introduced to wild-type potato Karnico. Cell wall materials were extracted from Karnico and transgenic lines expressing β-galactosidase (β-Gal-14 mutant) or rhamnogalacturonan lyase (RGL-18 mutant). After sequential extraction, β-Gal-14 hot buffer-soluble solids (HBSS) of pectin contained 54% less galactose than Karnico HBSS, representing shorter galactan side chains. The individual pectin populations of β-Gal-14 HBSS showed different modifications extended to the two sub-populations as obtained by ion-exchange chromatography. Compared to wild-type, RGL-18 HBSS contained 27% more galacturonic acid and 55% less Gal on fresh potato weight basis, which was due to the removal of galactan-rich rhamnogalacturonan I (RG-I) segments. All pectin populations of RGL-18 showed consistently low levels of RG-I segments. Transgenic modification showed side effects on the methyl-esterification and acetyl substitution of RGL-18 HBSS (DM = 53, DA = 21), but not of the β-Gal-14 HBSS in comparison to wild-type (DM = 29, DA = 54).

AB - Genes encoding pectic enzymes were introduced to wild-type potato Karnico. Cell wall materials were extracted from Karnico and transgenic lines expressing β-galactosidase (β-Gal-14 mutant) or rhamnogalacturonan lyase (RGL-18 mutant). After sequential extraction, β-Gal-14 hot buffer-soluble solids (HBSS) of pectin contained 54% less galactose than Karnico HBSS, representing shorter galactan side chains. The individual pectin populations of β-Gal-14 HBSS showed different modifications extended to the two sub-populations as obtained by ion-exchange chromatography. Compared to wild-type, RGL-18 HBSS contained 27% more galacturonic acid and 55% less Gal on fresh potato weight basis, which was due to the removal of galactan-rich rhamnogalacturonan I (RG-I) segments. All pectin populations of RGL-18 showed consistently low levels of RG-I segments. Transgenic modification showed side effects on the methyl-esterification and acetyl substitution of RGL-18 HBSS (DM = 53, DA = 21), but not of the β-Gal-14 HBSS in comparison to wild-type (DM = 29, DA = 54).

KW - Galactan side chains

KW - Plant cell wall polysaccharides

KW - Potato mutant

KW - Potato pectin

KW - Solanum tuberosum

KW - Starch removal

U2 - 10.1016/j.carbpol.2016.02.037

DO - 10.1016/j.carbpol.2016.02.037

M3 - Article

VL - 144

SP - 9

EP - 16

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

ER -