Moderate salinity improves stomatal functioning in rose plants grown at high relative air humidity

Dália R.A. Carvalho, Marta W. Vasconcelos, Sang Lee, Dick Vreugdenhil, Ep Heuvelink, Susana Pinto de Carvalho

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Plants grown at high relative air humidity (RH ≥ 85%) show hampered stomatal closure in response to closing stimuli. We hypothesized that a moderate salinity during growth could trigger a stress response and stimulate stomatal functioning due to an increased leaf abscisic acid concentration ([ABA]). Cut rose ‘Prophyta’ was grown at moderate (63%) or high (89%) RH combined with three electrical conductivities (EC) in the nutrient solution (2, 4 and 6 dS m−1; adding NaCl). High RH resulted in higher pore area per leaf area in intact leaves, and higher stomatal conductance (gs) both in leaves subjected to desiccation and to light/dark transition, as compared to moderate RH. Increasing EC in high RH-grown plants lead to higher stomatal density but it enhanced stomatal closure in response to leaflet desiccation. This enhanced stomatal functioning was associated with increased [ABA] and [ABA + metabolites]. Nonetheless, plants grown at EC6 showed a significantly lower chlorophyll content, total plant dry weight and total leaf area. This negative effect on plant growth is related to ionic stress as the sodium and chloride concentrations increased in plants grown at EC6 compared to EC2 (up to 111- and 14-fold, respectively). This is the first study on the interactive effects of RH and salinity on stomatal functioning and anatomy during leaf development. It is shown that, when these two environmental factors that influence stomatal responsiveness in an opposite way are combined, moderate EC is able to improve stomatal responsiveness to leaflet desiccation in high RH-grown plants due to increased leaf [ABA].
Original languageEnglish
Pages (from-to)1-9
JournalEnvironmental and Experimental Botany
Volume143
DOIs
Publication statusPublished - 2017

Fingerprint

humidity
Rosa
abscisic acid
salinity
air
desiccation (plant physiology)
electrical conductivity
desiccation
leaves
leaf area
leaf development
sodium chloride
nutrient solutions
stomatal conductance
stress response
chlorides
anatomy
sodium
plant growth
metabolite

Keywords

  • Abscisic acid
  • Rosa × hybrida
  • Salt stress
  • Stomatal physiology
  • Vapor pressure deficit
  • Water loss

Cite this

Carvalho, Dália R.A. ; Vasconcelos, Marta W. ; Lee, Sang ; Vreugdenhil, Dick ; Heuvelink, Ep ; Pinto de Carvalho, Susana. / Moderate salinity improves stomatal functioning in rose plants grown at high relative air humidity. In: Environmental and Experimental Botany. 2017 ; Vol. 143. pp. 1-9.
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title = "Moderate salinity improves stomatal functioning in rose plants grown at high relative air humidity",
abstract = "Plants grown at high relative air humidity (RH ≥ 85{\%}) show hampered stomatal closure in response to closing stimuli. We hypothesized that a moderate salinity during growth could trigger a stress response and stimulate stomatal functioning due to an increased leaf abscisic acid concentration ([ABA]). Cut rose ‘Prophyta’ was grown at moderate (63{\%}) or high (89{\%}) RH combined with three electrical conductivities (EC) in the nutrient solution (2, 4 and 6 dS m−1; adding NaCl). High RH resulted in higher pore area per leaf area in intact leaves, and higher stomatal conductance (gs) both in leaves subjected to desiccation and to light/dark transition, as compared to moderate RH. Increasing EC in high RH-grown plants lead to higher stomatal density but it enhanced stomatal closure in response to leaflet desiccation. This enhanced stomatal functioning was associated with increased [ABA] and [ABA + metabolites]. Nonetheless, plants grown at EC6 showed a significantly lower chlorophyll content, total plant dry weight and total leaf area. This negative effect on plant growth is related to ionic stress as the sodium and chloride concentrations increased in plants grown at EC6 compared to EC2 (up to 111- and 14-fold, respectively). This is the first study on the interactive effects of RH and salinity on stomatal functioning and anatomy during leaf development. It is shown that, when these two environmental factors that influence stomatal responsiveness in an opposite way are combined, moderate EC is able to improve stomatal responsiveness to leaflet desiccation in high RH-grown plants due to increased leaf [ABA].",
keywords = "Abscisic acid, Rosa × hybrida, Salt stress, Stomatal physiology, Vapor pressure deficit, Water loss",
author = "Carvalho, {D{\'a}lia R.A.} and Vasconcelos, {Marta W.} and Sang Lee and Dick Vreugdenhil and Ep Heuvelink and {Pinto de Carvalho}, Susana",
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language = "English",
volume = "143",
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journal = "Environmental and Experimental Botany",
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Moderate salinity improves stomatal functioning in rose plants grown at high relative air humidity. / Carvalho, Dália R.A.; Vasconcelos, Marta W.; Lee, Sang; Vreugdenhil, Dick; Heuvelink, Ep; Pinto de Carvalho, Susana.

In: Environmental and Experimental Botany, Vol. 143, 2017, p. 1-9.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Moderate salinity improves stomatal functioning in rose plants grown at high relative air humidity

AU - Carvalho, Dália R.A.

AU - Vasconcelos, Marta W.

AU - Lee, Sang

AU - Vreugdenhil, Dick

AU - Heuvelink, Ep

AU - Pinto de Carvalho, Susana

PY - 2017

Y1 - 2017

N2 - Plants grown at high relative air humidity (RH ≥ 85%) show hampered stomatal closure in response to closing stimuli. We hypothesized that a moderate salinity during growth could trigger a stress response and stimulate stomatal functioning due to an increased leaf abscisic acid concentration ([ABA]). Cut rose ‘Prophyta’ was grown at moderate (63%) or high (89%) RH combined with three electrical conductivities (EC) in the nutrient solution (2, 4 and 6 dS m−1; adding NaCl). High RH resulted in higher pore area per leaf area in intact leaves, and higher stomatal conductance (gs) both in leaves subjected to desiccation and to light/dark transition, as compared to moderate RH. Increasing EC in high RH-grown plants lead to higher stomatal density but it enhanced stomatal closure in response to leaflet desiccation. This enhanced stomatal functioning was associated with increased [ABA] and [ABA + metabolites]. Nonetheless, plants grown at EC6 showed a significantly lower chlorophyll content, total plant dry weight and total leaf area. This negative effect on plant growth is related to ionic stress as the sodium and chloride concentrations increased in plants grown at EC6 compared to EC2 (up to 111- and 14-fold, respectively). This is the first study on the interactive effects of RH and salinity on stomatal functioning and anatomy during leaf development. It is shown that, when these two environmental factors that influence stomatal responsiveness in an opposite way are combined, moderate EC is able to improve stomatal responsiveness to leaflet desiccation in high RH-grown plants due to increased leaf [ABA].

AB - Plants grown at high relative air humidity (RH ≥ 85%) show hampered stomatal closure in response to closing stimuli. We hypothesized that a moderate salinity during growth could trigger a stress response and stimulate stomatal functioning due to an increased leaf abscisic acid concentration ([ABA]). Cut rose ‘Prophyta’ was grown at moderate (63%) or high (89%) RH combined with three electrical conductivities (EC) in the nutrient solution (2, 4 and 6 dS m−1; adding NaCl). High RH resulted in higher pore area per leaf area in intact leaves, and higher stomatal conductance (gs) both in leaves subjected to desiccation and to light/dark transition, as compared to moderate RH. Increasing EC in high RH-grown plants lead to higher stomatal density but it enhanced stomatal closure in response to leaflet desiccation. This enhanced stomatal functioning was associated with increased [ABA] and [ABA + metabolites]. Nonetheless, plants grown at EC6 showed a significantly lower chlorophyll content, total plant dry weight and total leaf area. This negative effect on plant growth is related to ionic stress as the sodium and chloride concentrations increased in plants grown at EC6 compared to EC2 (up to 111- and 14-fold, respectively). This is the first study on the interactive effects of RH and salinity on stomatal functioning and anatomy during leaf development. It is shown that, when these two environmental factors that influence stomatal responsiveness in an opposite way are combined, moderate EC is able to improve stomatal responsiveness to leaflet desiccation in high RH-grown plants due to increased leaf [ABA].

KW - Abscisic acid

KW - Rosa × hybrida

KW - Salt stress

KW - Stomatal physiology

KW - Vapor pressure deficit

KW - Water loss

U2 - 10.1016/j.envexpbot.2017.07.018

DO - 10.1016/j.envexpbot.2017.07.018

M3 - Article

VL - 143

SP - 1

EP - 9

JO - Environmental and Experimental Botany

JF - Environmental and Experimental Botany

SN - 0098-8472

ER -