Moderate alcohol consumption alters both leucocyte gene expression profiles and circulating proteins related to immune response and lipid metabolism in men

M.M. Joosten, M.J. van Erk, E.P.M. Pellis, R.F. Witkamp, H.F.J. Hendriks

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

Moderate alcohol consumption has various effects on immune and inflammatory processes, which could accumulatively modulate chronic disease risk. So far, no comprehensive, integrative profiling has been performed to investigate the effects of longer-term alcohol consumption. Therefore, we studied the effects of alcohol consumption on gene expression patterns using large-scale profiling of whole-genome transcriptomics in blood cells and on a number of proteins in blood. In a randomised, open-label, cross-over trial, twenty-four young, normal-weight men consumed 100 ml vodka (30 g alcohol) with 200 ml orange juice or only orange juice daily during dinner for 4 weeks. After each period, blood was sampled for measuring gene expression and selected proteins. Pathway analysis of 345 down-regulated and 455 up-regulated genes revealed effects of alcohol consumption on various signalling responses, immune processes and lipid metabolism. Among the signalling processes, the most prominently changed was glucocorticoid receptor signalling. A network on immune response showed a down-regulated NF-¿B gene expression together with increased plasma adiponectin and decreased pro-inflammatory IL-1 receptor antagonist and IL-18, and acute-phase proteins ferritin and a1-antitrypsin concentrations (all P <0·05) after alcohol consumption. Furthermore, a network of gene expression changes related to lipid metabolism was observed, with a central role for PPARa which was supported by increased HDL-cholesterol and several apo concentrations (all P <0·05) after alcohol consumption. In conclusion, an integrated approach of profiling both genes and proteins in blood showed that 4 weeks of moderate alcohol consumption altered immune responses and lipid metabolism
Original languageEnglish
Pages (from-to)620-627
JournalBritish Journal of Nutrition
Volume108
Issue number4
DOIs
Publication statusPublished - 2012

Fingerprint

Lipid Metabolism
Transcriptome
Alcohol Drinking
Leukocytes
Proteins
Gene Expression
Interleukin-18
Interleukin-1 Receptors
Acute-Phase Proteins
Adiponectin
Glucocorticoid Receptors
Ferritins
Cross-Over Studies
HDL Cholesterol
Meals
Blood Proteins
Blood Cells
Chronic Disease
Alcohols
Genome

Keywords

  • coronary-heart-disease
  • c-reactive protein
  • factor-kappa-b
  • inflammatory markers
  • insulin sensitivity
  • iron stores
  • risk
  • adiponectin
  • women
  • metaanalysis

Cite this

@article{667f0599207746aab397d46caa604dfc,
title = "Moderate alcohol consumption alters both leucocyte gene expression profiles and circulating proteins related to immune response and lipid metabolism in men",
abstract = "Moderate alcohol consumption has various effects on immune and inflammatory processes, which could accumulatively modulate chronic disease risk. So far, no comprehensive, integrative profiling has been performed to investigate the effects of longer-term alcohol consumption. Therefore, we studied the effects of alcohol consumption on gene expression patterns using large-scale profiling of whole-genome transcriptomics in blood cells and on a number of proteins in blood. In a randomised, open-label, cross-over trial, twenty-four young, normal-weight men consumed 100 ml vodka (30 g alcohol) with 200 ml orange juice or only orange juice daily during dinner for 4 weeks. After each period, blood was sampled for measuring gene expression and selected proteins. Pathway analysis of 345 down-regulated and 455 up-regulated genes revealed effects of alcohol consumption on various signalling responses, immune processes and lipid metabolism. Among the signalling processes, the most prominently changed was glucocorticoid receptor signalling. A network on immune response showed a down-regulated NF-¿B gene expression together with increased plasma adiponectin and decreased pro-inflammatory IL-1 receptor antagonist and IL-18, and acute-phase proteins ferritin and a1-antitrypsin concentrations (all P <0·05) after alcohol consumption. Furthermore, a network of gene expression changes related to lipid metabolism was observed, with a central role for PPARa which was supported by increased HDL-cholesterol and several apo concentrations (all P <0·05) after alcohol consumption. In conclusion, an integrated approach of profiling both genes and proteins in blood showed that 4 weeks of moderate alcohol consumption altered immune responses and lipid metabolism",
keywords = "coronary-heart-disease, c-reactive protein, factor-kappa-b, inflammatory markers, insulin sensitivity, iron stores, risk, adiponectin, women, metaanalysis",
author = "M.M. Joosten and {van Erk}, M.J. and E.P.M. Pellis and R.F. Witkamp and H.F.J. Hendriks",
year = "2012",
doi = "10.1017/S0007114511005988",
language = "English",
volume = "108",
pages = "620--627",
journal = "The British journal of nutrition",
issn = "0007-1145",
publisher = "Cambridge University Press",
number = "4",

}

Moderate alcohol consumption alters both leucocyte gene expression profiles and circulating proteins related to immune response and lipid metabolism in men. / Joosten, M.M.; van Erk, M.J.; Pellis, E.P.M.; Witkamp, R.F.; Hendriks, H.F.J.

In: British Journal of Nutrition, Vol. 108, No. 4, 2012, p. 620-627.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Moderate alcohol consumption alters both leucocyte gene expression profiles and circulating proteins related to immune response and lipid metabolism in men

AU - Joosten, M.M.

AU - van Erk, M.J.

AU - Pellis, E.P.M.

AU - Witkamp, R.F.

AU - Hendriks, H.F.J.

PY - 2012

Y1 - 2012

N2 - Moderate alcohol consumption has various effects on immune and inflammatory processes, which could accumulatively modulate chronic disease risk. So far, no comprehensive, integrative profiling has been performed to investigate the effects of longer-term alcohol consumption. Therefore, we studied the effects of alcohol consumption on gene expression patterns using large-scale profiling of whole-genome transcriptomics in blood cells and on a number of proteins in blood. In a randomised, open-label, cross-over trial, twenty-four young, normal-weight men consumed 100 ml vodka (30 g alcohol) with 200 ml orange juice or only orange juice daily during dinner for 4 weeks. After each period, blood was sampled for measuring gene expression and selected proteins. Pathway analysis of 345 down-regulated and 455 up-regulated genes revealed effects of alcohol consumption on various signalling responses, immune processes and lipid metabolism. Among the signalling processes, the most prominently changed was glucocorticoid receptor signalling. A network on immune response showed a down-regulated NF-¿B gene expression together with increased plasma adiponectin and decreased pro-inflammatory IL-1 receptor antagonist and IL-18, and acute-phase proteins ferritin and a1-antitrypsin concentrations (all P <0·05) after alcohol consumption. Furthermore, a network of gene expression changes related to lipid metabolism was observed, with a central role for PPARa which was supported by increased HDL-cholesterol and several apo concentrations (all P <0·05) after alcohol consumption. In conclusion, an integrated approach of profiling both genes and proteins in blood showed that 4 weeks of moderate alcohol consumption altered immune responses and lipid metabolism

AB - Moderate alcohol consumption has various effects on immune and inflammatory processes, which could accumulatively modulate chronic disease risk. So far, no comprehensive, integrative profiling has been performed to investigate the effects of longer-term alcohol consumption. Therefore, we studied the effects of alcohol consumption on gene expression patterns using large-scale profiling of whole-genome transcriptomics in blood cells and on a number of proteins in blood. In a randomised, open-label, cross-over trial, twenty-four young, normal-weight men consumed 100 ml vodka (30 g alcohol) with 200 ml orange juice or only orange juice daily during dinner for 4 weeks. After each period, blood was sampled for measuring gene expression and selected proteins. Pathway analysis of 345 down-regulated and 455 up-regulated genes revealed effects of alcohol consumption on various signalling responses, immune processes and lipid metabolism. Among the signalling processes, the most prominently changed was glucocorticoid receptor signalling. A network on immune response showed a down-regulated NF-¿B gene expression together with increased plasma adiponectin and decreased pro-inflammatory IL-1 receptor antagonist and IL-18, and acute-phase proteins ferritin and a1-antitrypsin concentrations (all P <0·05) after alcohol consumption. Furthermore, a network of gene expression changes related to lipid metabolism was observed, with a central role for PPARa which was supported by increased HDL-cholesterol and several apo concentrations (all P <0·05) after alcohol consumption. In conclusion, an integrated approach of profiling both genes and proteins in blood showed that 4 weeks of moderate alcohol consumption altered immune responses and lipid metabolism

KW - coronary-heart-disease

KW - c-reactive protein

KW - factor-kappa-b

KW - inflammatory markers

KW - insulin sensitivity

KW - iron stores

KW - risk

KW - adiponectin

KW - women

KW - metaanalysis

U2 - 10.1017/S0007114511005988

DO - 10.1017/S0007114511005988

M3 - Article

VL - 108

SP - 620

EP - 627

JO - The British journal of nutrition

JF - The British journal of nutrition

SN - 0007-1145

IS - 4

ER -