Modelling of direct-transfer vitrification of bovine embryos

J. Lamy, A. de Wit, F. Fotinga-Meiring, E. Mullaart, L. Zak, E. Knol, E. Ellen, H. Woelders

Research output: Chapter in Book/Report/Conference proceedingConference paperAcademic

Abstract

Development of an effective direct transfer vitrification procedure for biopsied in vitro derived embryos is necessary for large scale use in the field and increased genetic gain in the cattle industry. One possible reason for insufficient results to date may be the swelling of embryos following warming/transfer. We used mathematical modelling of the volume changes of embryos during and following vitrification protocols, to study the effect of different temperatures and concentrations of cryoprotective agents (CPAs) and non-permeant solutes. The simulations showed no effect of temperature on the level of post-warming swelling at the assumed parameterizations. However, adapted vitrification media with lower concentrations of CPAs and higher non-permeant solutes led to clearly lower swelling after direct transfer, and could potentially be less toxic for the embryos, but also led to a stronger shrinking during vitrification. The alternative media and protocol seem promising, but empirical experiments are needed to validate this approach.
Original languageEnglish
Title of host publicationProceedings of 12th World Congress on Genetics Applied to Livestock Production (WCGALP)
Subtitle of host publicationTechnical and species orientated innovations in animal breeding, and contribution of genetics to solving societal challenges
EditorsR.F. Veerkamp, Y. de Haas
Place of PublicationWageningen
PublisherWageningen Academic Publishers
Pages2016-2019
ISBN (Electronic)9789086869404
DOIs
Publication statusPublished - 2022
EventWorld Congress on Genetics Applied to Livestock Production: WCGALP 2022 - Rotterdam, Netherlands
Duration: 3 Jul 20228 Jul 2022

Conference

ConferenceWorld Congress on Genetics Applied to Livestock Production: WCGALP 2022
Country/TerritoryNetherlands
CityRotterdam
Period3/07/228/07/22

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