Modeling the competition between antenna size mutant and wild type microalgae in outdoor mass culture

Tim de Mooij*, Kira Schediwy, René H. Wijffels, Marcel Janssen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Under high light conditions, microalgae are oversaturated with light which significantly reduces the light use efficiency. Microalgae with a reduced pigment content, antenna size mutants, have been proposed as a potential solution to increase the light use efficiency. The goal of this study was to investigate the competition between antenna size mutants and wild type microalgae in mass cultures. Using a kinetic model and literature-derived experimental data from wild type Chlorella sorokiniana, the productivity and competition of wild type cells and antenna size mutants were simulated. Cultivation was simulated in an outdoor microalgal raceway pond production system which was assumed to be limited by light only. Light conditions were based on a Mediterranean location (Tunisia) and a more temperate location (the Netherlands). Several wild type contamination levels were simulated in each mutant culture separately to predict the effect on the productivity over the cultivation time of a hypothetical summer season of 100 days. The simulations demonstrate a good potential of antenna size reduction to increase the biomass productivity of microalgal cultures. However, it was also found that after a contamination with wild type cells the mutant cultures will be rapidly overgrown resulting in productivity loss.

Original languageEnglish
Pages (from-to)1-13
JournalJournal of Biotechnology
Volume240
DOIs
Publication statusPublished - 2016

Fingerprint

Microalgae
Antennas
Light
Productivity
Contamination
Chlorella
Tunisia
Ponds
Cell Size
Pigments
Netherlands
Biomass
Cell Culture Techniques
Kinetics

Keywords

  • Antenna size mutant
  • Areal biomass productivity
  • Chlorella sorokiniana
  • Competition
  • Photosynthetic efficiency

Cite this

@article{ca674fa39cc240d4bebacafff9d71d7f,
title = "Modeling the competition between antenna size mutant and wild type microalgae in outdoor mass culture",
abstract = "Under high light conditions, microalgae are oversaturated with light which significantly reduces the light use efficiency. Microalgae with a reduced pigment content, antenna size mutants, have been proposed as a potential solution to increase the light use efficiency. The goal of this study was to investigate the competition between antenna size mutants and wild type microalgae in mass cultures. Using a kinetic model and literature-derived experimental data from wild type Chlorella sorokiniana, the productivity and competition of wild type cells and antenna size mutants were simulated. Cultivation was simulated in an outdoor microalgal raceway pond production system which was assumed to be limited by light only. Light conditions were based on a Mediterranean location (Tunisia) and a more temperate location (the Netherlands). Several wild type contamination levels were simulated in each mutant culture separately to predict the effect on the productivity over the cultivation time of a hypothetical summer season of 100 days. The simulations demonstrate a good potential of antenna size reduction to increase the biomass productivity of microalgal cultures. However, it was also found that after a contamination with wild type cells the mutant cultures will be rapidly overgrown resulting in productivity loss.",
keywords = "Antenna size mutant, Areal biomass productivity, Chlorella sorokiniana, Competition, Photosynthetic efficiency",
author = "{de Mooij}, Tim and Kira Schediwy and Wijffels, {Ren{\'e} H.} and Marcel Janssen",
year = "2016",
doi = "10.1016/j.jbiotec.2016.10.009",
language = "English",
volume = "240",
pages = "1--13",
journal = "Journal of Biotechnology",
issn = "0168-1656",
publisher = "Elsevier",

}

Modeling the competition between antenna size mutant and wild type microalgae in outdoor mass culture. / de Mooij, Tim; Schediwy, Kira; Wijffels, René H.; Janssen, Marcel.

In: Journal of Biotechnology, Vol. 240, 2016, p. 1-13.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Modeling the competition between antenna size mutant and wild type microalgae in outdoor mass culture

AU - de Mooij, Tim

AU - Schediwy, Kira

AU - Wijffels, René H.

AU - Janssen, Marcel

PY - 2016

Y1 - 2016

N2 - Under high light conditions, microalgae are oversaturated with light which significantly reduces the light use efficiency. Microalgae with a reduced pigment content, antenna size mutants, have been proposed as a potential solution to increase the light use efficiency. The goal of this study was to investigate the competition between antenna size mutants and wild type microalgae in mass cultures. Using a kinetic model and literature-derived experimental data from wild type Chlorella sorokiniana, the productivity and competition of wild type cells and antenna size mutants were simulated. Cultivation was simulated in an outdoor microalgal raceway pond production system which was assumed to be limited by light only. Light conditions were based on a Mediterranean location (Tunisia) and a more temperate location (the Netherlands). Several wild type contamination levels were simulated in each mutant culture separately to predict the effect on the productivity over the cultivation time of a hypothetical summer season of 100 days. The simulations demonstrate a good potential of antenna size reduction to increase the biomass productivity of microalgal cultures. However, it was also found that after a contamination with wild type cells the mutant cultures will be rapidly overgrown resulting in productivity loss.

AB - Under high light conditions, microalgae are oversaturated with light which significantly reduces the light use efficiency. Microalgae with a reduced pigment content, antenna size mutants, have been proposed as a potential solution to increase the light use efficiency. The goal of this study was to investigate the competition between antenna size mutants and wild type microalgae in mass cultures. Using a kinetic model and literature-derived experimental data from wild type Chlorella sorokiniana, the productivity and competition of wild type cells and antenna size mutants were simulated. Cultivation was simulated in an outdoor microalgal raceway pond production system which was assumed to be limited by light only. Light conditions were based on a Mediterranean location (Tunisia) and a more temperate location (the Netherlands). Several wild type contamination levels were simulated in each mutant culture separately to predict the effect on the productivity over the cultivation time of a hypothetical summer season of 100 days. The simulations demonstrate a good potential of antenna size reduction to increase the biomass productivity of microalgal cultures. However, it was also found that after a contamination with wild type cells the mutant cultures will be rapidly overgrown resulting in productivity loss.

KW - Antenna size mutant

KW - Areal biomass productivity

KW - Chlorella sorokiniana

KW - Competition

KW - Photosynthetic efficiency

U2 - 10.1016/j.jbiotec.2016.10.009

DO - 10.1016/j.jbiotec.2016.10.009

M3 - Article

VL - 240

SP - 1

EP - 13

JO - Journal of Biotechnology

JF - Journal of Biotechnology

SN - 0168-1656

ER -