Model description of bactrial 3-methylcatechol production in one- and two-phase systems

L.E. Husken, J. Hoogakker, J.A.M. de Bont, J. Tramper, H.H. Beeftink

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)


Pseudomonas putida MC2 produces 3-methylcatechol from toluene in aqueous medium. A second phase of 1-octanol may improve total product accumulation. To optimise the design of such a biphasic process, a process model was developed, both for one- and two-phase applications. The insights obtained by the model predictions showed the importance of different process parameters (like growth substrate concentration and partition coefficient) on growth of biomass, accumulation of 3-methylcatechol and processing time. For future applications, the process model can be used to ensure enough extraction capacity from aqueous to octanol phase. It is a useful tool to define the optimum process conditions, depending on the desired optimisation parameter: product concentration or processing time.
Original languageEnglish
Pages (from-to)11-17
JournalBioprocess and Biosystems Engineering
Publication statusPublished - 2003


  • pseudomonas-putida
  • organic-solvent
  • bioproduction
  • catechols

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