Mobile colistin resistance gene mcr-1 is detected on an Incl1 plasmid in E.coli from meat

Mike Brouwer*, Richard N. Goodman, A. Kant, D.J. Mevius, Enas Newire, Adam P. Roberts, K.T. Veldman

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)


Objectives: Mobile colistin resistance (mcr) genes encoded on conjugative plasmids, although described only relatively recently, have been reported globally both in humans and livestock. The genes are often associated with the insertion sequence ISApl1 that can transpose the genes to novel genetic locations. Since its first report, multiple variants of mcr have been discovered in a variety of genetic locations in Escherichia coli, in plasmids and integrated into the chromosome.
Methods: Using hybrid assembly of short-read and long-read whole-genome sequencing data, the presence ofmcr-1 was confirmed on an IncI1 plasmid in E. coli. In vitro conjugation assays were performed to determine the potential to transfer between strains. Genetic comparison with previously reported IncI1 plasmids was performed.
Results: The genomic sequence identified thatmcr-1 is present on a complete IncI1 plasmid. Comparison with previously reported extended-spectrum β-lactamase (ESBL)-encoding plasmids from E. coli in the Netherlands from the same time period indicated a distinct lineage for this plasmid.
Conclusions: The observation ofmcr-1 on an IncI1 plasmid confirms that the genetic region of this gene is actively transposed between genetic locations. This active transposition has consequences for the study of the epidemiology of mcr in populations.
Original languageEnglish
Pages (from-to)145-148
JournalJournal of Global Antimicrobial Resistance
Early online date2 Sept 2020
Publication statusPublished - Dec 2020


  • 1 mcr-1
  • Conjugation
  • IncI1 plasmid
  • Mobile colistin resistance


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