Projects per year
Methods: Using hybrid assembly of short-read and long-read whole-genome sequencing data, the presence ofmcr-1 was confirmed on an IncI1 plasmid in E. coli. In vitro conjugation assays were performed to determine the potential to transfer between strains. Genetic comparison with previously reported IncI1 plasmids was performed.
Results: The genomic sequence identified thatmcr-1 is present on a complete IncI1 plasmid. Comparison with previously reported extended-spectrum β-lactamase (ESBL)-encoding plasmids from E. coli in the Netherlands from the same time period indicated a distinct lineage for this plasmid.
Conclusions: The observation ofmcr-1 on an IncI1 plasmid confirms that the genetic region of this gene is actively transposed between genetic locations. This active transposition has consequences for the study of the epidemiology of mcr in populations.
- 1 mcr-1
- IncI1 plasmid
- Mobile colistin resistance
FingerprintDive into the research topics of 'Mobile colistin resistance gene mcr-1 is detected on an Incl1 plasmid in E.coli from meat'. Together they form a unique fingerprint.
One Health EJP: Promoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards.
1/01/18 → 31/12/22
Project: EU research project
1/01/08 → 31/12/11