Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

Robert M. Bowers; Nikos C. Kyrpides; Ramunas Stepanauskas; Miranda Harmon-Smith; Devin Doud; T.B.K. Reddy; Frederik Schulz; Jessica Jarett; Adam R. Rivers; Emiley A. Eloe-Fadrosh; Susannah G. Tringe; Natalia N. Ivanova; Alex Copeland; Alicia Clum; Eric D. Becraft; Rex R. Malmstrom; Bruce Birren; Mircea Podar; Peer Bork; George M. Weinstock; George M. Garrity; Jeremy A. Dodsworth; Shibu Yooseph; Granger Sutton; Frank O. Glöckner; Jack A. Gilbert; William C. Nelson; Steven J. Hallam; Sean P. Jungbluth; Thijs J.G. Ettema; Scott Tighe; Konstantinos T. Konstantinidis; Wen Tso Liu; Brett J. Baker; Thomas Rattei; Jonathan A. Eisen; Brian Hedlund; Katherine D. McMahon; Noah Fierer; Rob Knight; Rob Finn; Guy Cochrane; Ilene Karsch-Mizrachi; Gene W. Tyson; Christian Rinke; Alla Lapidus; Folker Meyer; Pelin Yilmaz; Donovan H. Parks; A.M. Eren

doi:10.1038/nbt.3893

Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

Robert M. Bowers^*, Nikos C. Kyrpides, Ramunas Stepanauskas, Miranda Harmon-Smith, Devin Doud, T.B.K. Reddy, Frederik Schulz, Jessica Jarett, Adam R. Rivers, Emiley A. Eloe-Fadrosh, Susannah G. Tringe, Natalia N. Ivanova, Alex Copeland, Alicia Clum, Eric D. Becraft, Rex R. Malmstrom, Bruce Birren, Mircea Podar, Peer Bork, George M. WeinstockGeorge M. Garrity, Jeremy A. Dodsworth, Shibu Yooseph, Granger Sutton, Frank O. Glöckner, Jack A. Gilbert, William C. Nelson, Steven J. Hallam, Sean P. Jungbluth, Thijs J.G. Ettema, Scott Tighe, Konstantinos T. Konstantinidis, Wen Tso Liu, Brett J. Baker, Thomas Rattei, Jonathan A. Eisen, Brian Hedlund, Katherine D. McMahon, Noah Fierer, Rob Knight, Rob Finn, Guy Cochrane, Ilene Karsch-Mizrachi, Gene W. Tyson, Christian Rinke, Alla Lapidus, Folker Meyer, Pelin Yilmaz, Donovan H. Parks, A.M. Eren

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

750 Citations (Scopus)

Abstract

We present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.

Original language	English
Pages (from-to)	725-731
Number of pages	7
Journal	Nature Biotechnology
Volume	35
Issue number	8
DOIs	https://doi.org/10.1038/nbt.3893
Publication status	Published - 8 Aug 2017
Externally published	Yes

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Bowers, R. M., Kyrpides, N. C., Stepanauskas, R., Harmon-Smith, M., Doud, D., Reddy, T. B. K., Schulz, F., Jarett, J., Rivers, A. R., Eloe-Fadrosh, E. A., Tringe, S. G., Ivanova, N. N., Copeland, A., Clum, A., Becraft, E. D., Malmstrom, R. R., Birren, B., Podar, M., Bork, P., ... Eren, A. M. (2017). Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea. Nature Biotechnology, 35(8), 725-731. https://doi.org/10.1038/nbt.3893

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title = "Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea",

abstract = "We present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.",

author = "Bowers, {Robert M.} and Kyrpides, {Nikos C.} and Ramunas Stepanauskas and Miranda Harmon-Smith and Devin Doud and T.B.K. Reddy and Frederik Schulz and Jessica Jarett and Rivers, {Adam R.} and Eloe-Fadrosh, {Emiley A.} and Tringe, {Susannah G.} and Ivanova, {Natalia N.} and Alex Copeland and Alicia Clum and Becraft, {Eric D.} and Malmstrom, {Rex R.} and Bruce Birren and Mircea Podar and Peer Bork and Weinstock, {George M.} and Garrity, {George M.} and Dodsworth, {Jeremy A.} and Shibu Yooseph and Granger Sutton and Gl{\"o}ckner, {Frank O.} and Gilbert, {Jack A.} and Nelson, {William C.} and Hallam, {Steven J.} and Jungbluth, {Sean P.} and Ettema, {Thijs J.G.} and Scott Tighe and Konstantinidis, {Konstantinos T.} and Liu, {Wen Tso} and Baker, {Brett J.} and Thomas Rattei and Eisen, {Jonathan A.} and Brian Hedlund and McMahon, {Katherine D.} and Noah Fierer and Rob Knight and Rob Finn and Guy Cochrane and Ilene Karsch-Mizrachi and Tyson, {Gene W.} and Christian Rinke and Alla Lapidus and Folker Meyer and Pelin Yilmaz and Parks, {Donovan H.} and A.M. Eren",

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doi = "10.1038/nbt.3893",

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journal = "Nature Biotechnology",

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Bowers, RM, Kyrpides, NC, Stepanauskas, R, Harmon-Smith, M, Doud, D, Reddy, TBK, Schulz, F, Jarett, J, Rivers, AR, Eloe-Fadrosh, EA, Tringe, SG, Ivanova, NN, Copeland, A, Clum, A, Becraft, ED, Malmstrom, RR, Birren, B, Podar, M, Bork, P, Weinstock, GM, Garrity, GM, Dodsworth, JA, Yooseph, S, Sutton, G, Glöckner, FO, Gilbert, JA, Nelson, WC, Hallam, SJ, Jungbluth, SP, Ettema, TJG, Tighe, S, Konstantinidis, KT, Liu, WT, Baker, BJ, Rattei, T, Eisen, JA, Hedlund, B, McMahon, KD, Fierer, N, Knight, R, Finn, R, Cochrane, G, Karsch-Mizrachi, I, Tyson, GW, Rinke, C, Lapidus, A, Meyer, F, Yilmaz, P, Parks, DH & Eren, AM 2017, 'Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea', Nature Biotechnology, vol. 35, no. 8, pp. 725-731. https://doi.org/10.1038/nbt.3893

TY - JOUR

T1 - Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

AU - Bowers, Robert M.

AU - Kyrpides, Nikos C.

AU - Stepanauskas, Ramunas

AU - Harmon-Smith, Miranda

AU - Doud, Devin

AU - Reddy, T.B.K.

AU - Schulz, Frederik

AU - Jarett, Jessica

AU - Rivers, Adam R.

AU - Eloe-Fadrosh, Emiley A.

AU - Tringe, Susannah G.

AU - Ivanova, Natalia N.

AU - Copeland, Alex

AU - Clum, Alicia

AU - Becraft, Eric D.

AU - Malmstrom, Rex R.

AU - Birren, Bruce

AU - Podar, Mircea

AU - Bork, Peer

AU - Weinstock, George M.

AU - Garrity, George M.

AU - Dodsworth, Jeremy A.

AU - Yooseph, Shibu

AU - Sutton, Granger

AU - Glöckner, Frank O.

AU - Gilbert, Jack A.

AU - Nelson, William C.

AU - Hallam, Steven J.

AU - Jungbluth, Sean P.

AU - Ettema, Thijs J.G.

AU - Tighe, Scott

AU - Konstantinidis, Konstantinos T.

AU - Liu, Wen Tso

AU - Baker, Brett J.

AU - Rattei, Thomas

AU - Eisen, Jonathan A.

AU - Hedlund, Brian

AU - McMahon, Katherine D.

AU - Fierer, Noah

AU - Knight, Rob

AU - Finn, Rob

AU - Cochrane, Guy

AU - Karsch-Mizrachi, Ilene

AU - Tyson, Gene W.

AU - Rinke, Christian

AU - Lapidus, Alla

AU - Meyer, Folker

AU - Yilmaz, Pelin

AU - Parks, Donovan H.

AU - Eren, A.M.

PY - 2017/8/8

Y1 - 2017/8/8

N2 - We present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.

AB - We present two standards developed by the Genomic Standards Consortium (GSC) for reporting bacterial and archaeal genome sequences. Both are extensions of the Minimum Information about Any (x) Sequence (MIxS). The standards are the Minimum Information about a Single Amplified Genome (MISAG) and the Minimum Information about a Metagenome-Assembled Genome (MIMAG), including, but not limited to, assembly quality, and estimates of genome completeness and contamination. These standards can be used in combination with other GSC checklists, including the Minimum Information about a Genome Sequence (MIGS), Minimum Information about a Metagenomic Sequence (MIMS), and Minimum Information about a Marker Gene Sequence (MIMARKS). Community-wide adoption of MISAG and MIMAG will facilitate more robust comparative genomic analyses of bacterial and archaeal diversity.

U2 - 10.1038/nbt.3893

DO - 10.1038/nbt.3893

M3 - Article

C2 - 28787424

AN - SCOPUS:85027264358

SN - 1087-0156

VL - 35

SP - 725

EP - 731

JO - Nature Biotechnology

JF - Nature Biotechnology

IS - 8

ER -

Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea

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