Millifluidic droplet analyser for microbiology

L. Baraban, F. Bertholle, M.L.M. Salverda, N. Bremond, P. Panizza, J. Baudry, J.A.G.M. de Visser, J. Bibette

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

We present a novel millifluidic droplet analyser (MDA) for precisely monitoring the dynamics of microbial populations over multiple generations in numerous (=103) aqueous emulsion droplets (100 nL). As a first application, we measure the growth rate of a bacterial strain and determine the minimal inhibitory concentration (MIC) for the antibiotic cefotaxime by incubating bacteria in a fine gradient of antibiotic concentrations. The detection of cell activity is based on the automated detection of an epifluorescent signal that allows the monitoring of microbial populations up to a size of 106 cells. We believe that this device is helpful for the study of population dynamic consequences of microbe-environment interactions and of individual cell differences. Moreover, the fluidic machine may improve clinical tests, as it simplifies, automates and miniaturizes the screening of numerous microbial populations that grow and evolve in compartments with a finely tuned composition
Original languageEnglish
Pages (from-to)4057-4062
JournalLab on a Chip
Volume11
DOIs
Publication statusPublished - 2011

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Microbiology
Population Dynamics
Antibiotics
Anti-Bacterial Agents
Population dynamics
Cefotaxime
Monitoring
Fluidics
Emulsions
Cell Size
Individuality
Cell Communication
Population
Bacteria
Screening
Equipment and Supplies
Growth
Chemical analysis
Psychological Signal Detection

Keywords

  • stochastic gene-expression
  • escherichia-coli
  • single cells
  • bacteria
  • susceptibility
  • resistance
  • microfluidics
  • encapsulation
  • consequences
  • antibiotics

Cite this

Baraban, L., Bertholle, F., Salverda, M. L. M., Bremond, N., Panizza, P., Baudry, J., ... Bibette, J. (2011). Millifluidic droplet analyser for microbiology. Lab on a Chip, 11, 4057-4062. https://doi.org/10.1039/c1lc20545e
Baraban, L. ; Bertholle, F. ; Salverda, M.L.M. ; Bremond, N. ; Panizza, P. ; Baudry, J. ; de Visser, J.A.G.M. ; Bibette, J. / Millifluidic droplet analyser for microbiology. In: Lab on a Chip. 2011 ; Vol. 11. pp. 4057-4062.
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abstract = "We present a novel millifluidic droplet analyser (MDA) for precisely monitoring the dynamics of microbial populations over multiple generations in numerous (=103) aqueous emulsion droplets (100 nL). As a first application, we measure the growth rate of a bacterial strain and determine the minimal inhibitory concentration (MIC) for the antibiotic cefotaxime by incubating bacteria in a fine gradient of antibiotic concentrations. The detection of cell activity is based on the automated detection of an epifluorescent signal that allows the monitoring of microbial populations up to a size of 106 cells. We believe that this device is helpful for the study of population dynamic consequences of microbe-environment interactions and of individual cell differences. Moreover, the fluidic machine may improve clinical tests, as it simplifies, automates and miniaturizes the screening of numerous microbial populations that grow and evolve in compartments with a finely tuned composition",
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Baraban, L, Bertholle, F, Salverda, MLM, Bremond, N, Panizza, P, Baudry, J, de Visser, JAGM & Bibette, J 2011, 'Millifluidic droplet analyser for microbiology', Lab on a Chip, vol. 11, pp. 4057-4062. https://doi.org/10.1039/c1lc20545e

Millifluidic droplet analyser for microbiology. / Baraban, L.; Bertholle, F.; Salverda, M.L.M.; Bremond, N.; Panizza, P.; Baudry, J.; de Visser, J.A.G.M.; Bibette, J.

In: Lab on a Chip, Vol. 11, 2011, p. 4057-4062.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Millifluidic droplet analyser for microbiology

AU - Baraban, L.

AU - Bertholle, F.

AU - Salverda, M.L.M.

AU - Bremond, N.

AU - Panizza, P.

AU - Baudry, J.

AU - de Visser, J.A.G.M.

AU - Bibette, J.

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AB - We present a novel millifluidic droplet analyser (MDA) for precisely monitoring the dynamics of microbial populations over multiple generations in numerous (=103) aqueous emulsion droplets (100 nL). As a first application, we measure the growth rate of a bacterial strain and determine the minimal inhibitory concentration (MIC) for the antibiotic cefotaxime by incubating bacteria in a fine gradient of antibiotic concentrations. The detection of cell activity is based on the automated detection of an epifluorescent signal that allows the monitoring of microbial populations up to a size of 106 cells. We believe that this device is helpful for the study of population dynamic consequences of microbe-environment interactions and of individual cell differences. Moreover, the fluidic machine may improve clinical tests, as it simplifies, automates and miniaturizes the screening of numerous microbial populations that grow and evolve in compartments with a finely tuned composition

KW - stochastic gene-expression

KW - escherichia-coli

KW - single cells

KW - bacteria

KW - susceptibility

KW - resistance

KW - microfluidics

KW - encapsulation

KW - consequences

KW - antibiotics

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DO - 10.1039/c1lc20545e

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JO - Lab on a Chip

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Baraban L, Bertholle F, Salverda MLM, Bremond N, Panizza P, Baudry J et al. Millifluidic droplet analyser for microbiology. Lab on a Chip. 2011;11:4057-4062. https://doi.org/10.1039/c1lc20545e