Mild acoustic processing of Tisochrysis lutea for multiproduct biorefineries

Iulian Boboescu*, Antoinette Kazbar, Lars Stegemüller, Piet Lazeroms, Thanasis Triantafyllou, Fengzheng Gao, Calvin Lo, Maria J. Barbosa, Michel H.M. Eppink, Rene H. Wijffels

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Cellular agriculture could represent a more sustainable alternative to current food and nutraceutical production processes. Tisochrysis lutea microalgae represents a rich source of antioxidants and omega-3 fatty acids essential for human health. However, current downstream technologies are limiting its use. The present work investigates mild targeted acoustic treatment of Tisochrysis lutea biomass at different growth stages and acoustic frequencies, intensities and treatment times. Significant differences have been observed in terms of the impact of these variables on the cell disruption and energy requirements. Lower frequencies of 20 kHz required a minimum of 4500 J to disrupt 90% of the cells, while only 1000 J at 1146 kHz. Comparing these results with current industry standards such as bead milling, up to six times less energy use has been identified. These mild biomass processing approaches offer a certain tunability which could suit a wide range of microorganisms with only minor adjustments.
Original languageEnglish
Article number127582
JournalBioresource Technology
Early online date4 Jul 2022
Publication statusPublished - Sept 2022


Dive into the research topics of 'Mild acoustic processing of Tisochrysis lutea for multiproduct biorefineries'. Together they form a unique fingerprint.

Cite this