Abstract
A defined template mixture of seven closely related 16S-rDNA clones was used in a PCR-cloning experiment to assess and track sources of artifactual sequence variation in 16S rDNA clone libraries. At least 14% of the recovered clones contained aberrations. Artifact sources were polymerase errors, a mutational hot spot, and cloning of heteroduplexes and chimeras. These data may partially explain the high degree of microheterogeneity typical of sequence clusters detected in environmental clone libraries
Original language | English |
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Pages (from-to) | 469-472 |
Journal | Applied and Environmental Microbiology |
Volume | 67 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2001 |
Keywords
- ribosomal-rna genes
- polymerase chain-reaction
- chimeric molecules
- dna amplification
- escherichia-coli
- heteroduplexes
- populations
- coamplification
- consequence
- diversity