Microsatellites within genes and ESTs of common carp and their applicability in silver crucian carp

G.H. Yue; M.Y. Ho; L. Orban; J. Komen

doi:10.1016/j.aquaculture.2003.12.021

Microsatellites within genes and ESTs of common carp and their applicability in silver crucian carp

G.H. Yue
, M.Y. Ho
, L. Orban
, J. Komen

Research output: Contribution to journal › Article › Academic › peer-review

76 Citations (Scopus)

Abstract

Thirty-six new microsatellites were identified from common carp (Cyprinus carpio L.) by screening through genes found in GenBank, EST sequences from a testis cDNA library and a genomic DNA library enriched for CA repeats. Eleven of the twenty-eight microsatellites identified from genes and ESTs were AT repeats, suggesting their high abundance in the genome of common carp. Characterization of the 36 microsatellites on a panel of 18 unrelated common carp individuals revealed that all, except two, were polymorphic with an average allele number of 7.3/locus (range: 2¿15 alleles/locus). The microsatellites located in genes and ESTs showed higher allele number than those eight which were isolated from a genomic DNA library (7.7/locus vs. 4.9/locus df=32, P

Original language	English
Pages (from-to)	85-98
Journal	Aquaculture
Volume	234
Issue number	1-4
DOIs	https://doi.org/10.1016/j.aquaculture.2003.12.021
Publication status	Published - 2004

Keywords

cross-species amplification
carassius-auratus gibelio
polymerase-chain-reaction
simple sequence repeats
cyprinus-carpio
dna polymorphisms
rapid isolation
compact genome
fresh-water
markers

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10.1016/j.aquaculture.2003.12.021

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title = "Microsatellites within genes and ESTs of common carp and their applicability in silver crucian carp",

abstract = "Thirty-six new microsatellites were identified from common carp (Cyprinus carpio L.) by screening through genes found in GenBank, EST sequences from a testis cDNA library and a genomic DNA library enriched for CA repeats. Eleven of the twenty-eight microsatellites identified from genes and ESTs were AT repeats, suggesting their high abundance in the genome of common carp. Characterization of the 36 microsatellites on a panel of 18 unrelated common carp individuals revealed that all, except two, were polymorphic with an average allele number of 7.3/locus (range: 2¿15 alleles/locus). The microsatellites located in genes and ESTs showed higher allele number than those eight which were isolated from a genomic DNA library (7.7/locus vs. 4.9/locus df=32, P",

keywords = "cross-species amplification, carassius-auratus gibelio, polymerase-chain-reaction, simple sequence repeats, cyprinus-carpio, dna polymorphisms, rapid isolation, compact genome, fresh-water, markers",

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T1 - Microsatellites within genes and ESTs of common carp and their applicability in silver crucian carp

AU - Yue, G.H.

AU - Ho, M.Y.

AU - Orban, L.

AU - Komen, J.

PY - 2004

Y1 - 2004

N2 - Thirty-six new microsatellites were identified from common carp (Cyprinus carpio L.) by screening through genes found in GenBank, EST sequences from a testis cDNA library and a genomic DNA library enriched for CA repeats. Eleven of the twenty-eight microsatellites identified from genes and ESTs were AT repeats, suggesting their high abundance in the genome of common carp. Characterization of the 36 microsatellites on a panel of 18 unrelated common carp individuals revealed that all, except two, were polymorphic with an average allele number of 7.3/locus (range: 2¿15 alleles/locus). The microsatellites located in genes and ESTs showed higher allele number than those eight which were isolated from a genomic DNA library (7.7/locus vs. 4.9/locus df=32, P

AB - Thirty-six new microsatellites were identified from common carp (Cyprinus carpio L.) by screening through genes found in GenBank, EST sequences from a testis cDNA library and a genomic DNA library enriched for CA repeats. Eleven of the twenty-eight microsatellites identified from genes and ESTs were AT repeats, suggesting their high abundance in the genome of common carp. Characterization of the 36 microsatellites on a panel of 18 unrelated common carp individuals revealed that all, except two, were polymorphic with an average allele number of 7.3/locus (range: 2¿15 alleles/locus). The microsatellites located in genes and ESTs showed higher allele number than those eight which were isolated from a genomic DNA library (7.7/locus vs. 4.9/locus df=32, P

KW - cross-species amplification

KW - carassius-auratus gibelio

KW - polymerase-chain-reaction

KW - simple sequence repeats

KW - cyprinus-carpio

KW - dna polymorphisms

KW - rapid isolation

KW - compact genome

KW - fresh-water

KW - markers

U2 - 10.1016/j.aquaculture.2003.12.021

DO - 10.1016/j.aquaculture.2003.12.021

M3 - Article

SN - 0044-8486

VL - 234

SP - 85

EP - 98

JO - Aquaculture

JF - Aquaculture

IS - 1-4

ER -

Microsatellites within genes and ESTs of common carp and their applicability in silver crucian carp

Abstract

Keywords

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