Microsatellites within genes and ESTs of common carp and their applicability in silver crucian carp

G.H. Yue, M.Y. Ho, L. Orban, J. Komen

Research output: Contribution to journalArticleAcademicpeer-review

69 Citations (Scopus)

Abstract

Thirty-six new microsatellites were identified from common carp (Cyprinus carpio L.) by screening through genes found in GenBank, EST sequences from a testis cDNA library and a genomic DNA library enriched for CA repeats. Eleven of the twenty-eight microsatellites identified from genes and ESTs were AT repeats, suggesting their high abundance in the genome of common carp. Characterization of the 36 microsatellites on a panel of 18 unrelated common carp individuals revealed that all, except two, were polymorphic with an average allele number of 7.3/locus (range: 2¿15 alleles/locus). The microsatellites located in genes and ESTs showed higher allele number than those eight which were isolated from a genomic DNA library (7.7/locus vs. 4.9/locus df=32, P
Original languageEnglish
Pages (from-to)85-98
JournalAquaculture
Volume234
Issue number1-4
DOIs
Publication statusPublished - 2004

Keywords

  • cross-species amplification
  • carassius-auratus gibelio
  • polymerase-chain-reaction
  • simple sequence repeats
  • cyprinus-carpio
  • dna polymorphisms
  • rapid isolation
  • compact genome
  • fresh-water
  • markers

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