Microsatellites of the parasitic nematode Haemonchus contortus: polymorphism and linkage with a direct repeat

R. Hoekstra, A. Criado-Fornelio, J. Fakkeldij, J. Bergman, M.H. Roos

    Research output: Contribution to journalArticleAcademicpeer-review

    55 Citations (Scopus)

    Abstract

    To develop tools to analyse parasitic nematode population structures and the effects of selection pressure on the nematode population, we isolated and characterised 13 microsatellite markers of the nematode Haemonchus contortus. The density of CA/GT microsatellites, once in 575 kb, is lower than in mammals. Although the isolated CA/GT repeats were imperfect, the majority of the microsatellites were polymorphic in one or more populations. An extensive genetic diversity both within and between populations could be established Two-thirds of the CA/GT microsatellites were followed by a variable number of 128 bp direct repeat elements, HcREP1. HcREP1 is a repetitive element in the H. contortus genome, which is homologous to the repetitive TcREP element in the nematode Trichostrongylus colubriformis.
    Original languageEnglish
    Pages (from-to)97-107
    JournalMolecular and biochemical parasitology
    Volume89
    Issue number1
    DOIs
    Publication statusPublished - 1997

    Fingerprint Dive into the research topics of 'Microsatellites of the parasitic nematode Haemonchus contortus: polymorphism and linkage with a direct repeat'. Together they form a unique fingerprint.

  • Cite this