Microsatellite variation and population structure of a recovering tree frog (Hyla arborea L.) metapopulation

P.F.P. Arens, R.J.F. Bugter, W.P.C. van 't Westende, R. Zollinger, J. Stronks, C.C. Vos, M.J.M. Smulders

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

Numbers and sizes of populations of the European tree frog in The Netherlands have dramatically decreased in the second half of the last century due to extensive habitat destruction and fragmentation. We have studied the genetic structure of a slowly recovering meta-population. Strong genetic differentiation, estimated at eight microsatellite loci, was found between clusters of populations (F (st)-values above 0.2). Within clusters, consisting of ponds within a radius of about 5 km, European tree frog populations were less differentiated (F (st)<0.08) and exact tests showed that most of the ponds within clusters were not significantly differentiated. Although local population sizes have been increasing since 1989, and some new ponds have been colonised in the direct vicinity of ponds that have been populated continuously, little evidence for gene flow between clusters of ponds was found (only one exception). Furthermore, levels of genetic diversity were low compared to populations in comparable areas elsewhere in Europe. Therefore, a continuous conservation effort is needed to prevent any further loss of genetic diversity. The alleviation of important barriers to dispersal between the clusters should be given a high priority for the restoration of the meta-population as a whole.
Original languageEnglish
Pages (from-to)825-835
JournalConservation Genetics
Volume7
Issue number6
DOIs
Publication statusPublished - 2006

Fingerprint

Hyla
Hylidae
metapopulation
frog
Anura
Microsatellite Repeats
population structure
pond
microsatellite repeats
Population
Population Density
genetic variation
population size
Gene Flow
Genetic Structures
habitat fragmentation
habitat destruction
genetic differentiation
Netherlands
genetic structure

Keywords

  • bottlenecks
  • differentiation
  • amphibians
  • declines
  • tests

Cite this

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title = "Microsatellite variation and population structure of a recovering tree frog (Hyla arborea L.) metapopulation",
abstract = "Numbers and sizes of populations of the European tree frog in The Netherlands have dramatically decreased in the second half of the last century due to extensive habitat destruction and fragmentation. We have studied the genetic structure of a slowly recovering meta-population. Strong genetic differentiation, estimated at eight microsatellite loci, was found between clusters of populations (F (st)-values above 0.2). Within clusters, consisting of ponds within a radius of about 5 km, European tree frog populations were less differentiated (F (st)<0.08) and exact tests showed that most of the ponds within clusters were not significantly differentiated. Although local population sizes have been increasing since 1989, and some new ponds have been colonised in the direct vicinity of ponds that have been populated continuously, little evidence for gene flow between clusters of ponds was found (only one exception). Furthermore, levels of genetic diversity were low compared to populations in comparable areas elsewhere in Europe. Therefore, a continuous conservation effort is needed to prevent any further loss of genetic diversity. The alleviation of important barriers to dispersal between the clusters should be given a high priority for the restoration of the meta-population as a whole.",
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Microsatellite variation and population structure of a recovering tree frog (Hyla arborea L.) metapopulation. / Arens, P.F.P.; Bugter, R.J.F.; van 't Westende, W.P.C.; Zollinger, R.; Stronks, J.; Vos, C.C.; Smulders, M.J.M.

In: Conservation Genetics, Vol. 7, No. 6, 2006, p. 825-835.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Microsatellite variation and population structure of a recovering tree frog (Hyla arborea L.) metapopulation

AU - Arens, P.F.P.

AU - Bugter, R.J.F.

AU - van 't Westende, W.P.C.

AU - Zollinger, R.

AU - Stronks, J.

AU - Vos, C.C.

AU - Smulders, M.J.M.

PY - 2006

Y1 - 2006

N2 - Numbers and sizes of populations of the European tree frog in The Netherlands have dramatically decreased in the second half of the last century due to extensive habitat destruction and fragmentation. We have studied the genetic structure of a slowly recovering meta-population. Strong genetic differentiation, estimated at eight microsatellite loci, was found between clusters of populations (F (st)-values above 0.2). Within clusters, consisting of ponds within a radius of about 5 km, European tree frog populations were less differentiated (F (st)<0.08) and exact tests showed that most of the ponds within clusters were not significantly differentiated. Although local population sizes have been increasing since 1989, and some new ponds have been colonised in the direct vicinity of ponds that have been populated continuously, little evidence for gene flow between clusters of ponds was found (only one exception). Furthermore, levels of genetic diversity were low compared to populations in comparable areas elsewhere in Europe. Therefore, a continuous conservation effort is needed to prevent any further loss of genetic diversity. The alleviation of important barriers to dispersal between the clusters should be given a high priority for the restoration of the meta-population as a whole.

AB - Numbers and sizes of populations of the European tree frog in The Netherlands have dramatically decreased in the second half of the last century due to extensive habitat destruction and fragmentation. We have studied the genetic structure of a slowly recovering meta-population. Strong genetic differentiation, estimated at eight microsatellite loci, was found between clusters of populations (F (st)-values above 0.2). Within clusters, consisting of ponds within a radius of about 5 km, European tree frog populations were less differentiated (F (st)<0.08) and exact tests showed that most of the ponds within clusters were not significantly differentiated. Although local population sizes have been increasing since 1989, and some new ponds have been colonised in the direct vicinity of ponds that have been populated continuously, little evidence for gene flow between clusters of ponds was found (only one exception). Furthermore, levels of genetic diversity were low compared to populations in comparable areas elsewhere in Europe. Therefore, a continuous conservation effort is needed to prevent any further loss of genetic diversity. The alleviation of important barriers to dispersal between the clusters should be given a high priority for the restoration of the meta-population as a whole.

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