Micropropagation of Ferula assa-foetida L. (a medicinal plant) via direct somatic embryogenesis

S. Roozbeh, M. Otroshy, R. Bozorgipoor, M. Ebrahimi, A. Moeini Najafabadi, P.C. Struik

Research output: Chapter in Book/Report/Conference proceedingConference paper

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Abstract

Ferula assa-foetida is a medicinal plant from the Apiaceae family. It is a monocarpic plant and only flowers once during its growth period and then it dries forever and that is one of the main problems of multiplication. The experiment was carried out to examine the effect of explants, medium and plant growth regulators, on the in vitro direct somatic embryogenesis of asafetida (Ferula assa-foetida). Explants of root, hypocotyl and cotyledon (leaf) were investigated on MS and B5 medium combined with 2,4-D (0.2, 0.5 or 1 mg/L), BAP (0.2, 0.5 or 1 mg/L) and NAA (0.2, 0.5 or 1 mg/L), each of them was combined with kin (0, 0.2, 0.5 or 1 mg/L). The best direct somatic embryogenesis was obtained from explants of leaves, no significant effect between explants of cotyledon and hypocotyls was found. 2,4-D (0.2 mg/L) + kin (0.2 mg/L) resulted in the highest percent of direct somatic embryogenesis, also the maximum indirect somatic embryogenesis was obtained from explants of root grown on the 2,4-D (0.5 mg/L) + kin (0.2 mg/L). The highest value for direct somatic embryogenesis was found in MS medium combined with 2,4-D (0.2 mg/L) + kin (0.2 mg/L), while the maximum of indirect somatic embryogenesis was observed in B5 medium enriched with 2,4-D (0.5 mg/L) + kin (0.2 mg/L). The maximum percentage of embryo conversion into seedling was found with 2,4-D (0.5 mg/L) + kin (0.2 mg/L) in B5. Highest percent of callogenesis was observed on B5 containing NAA (1 mg/L) + kin (1 mg/L), BAP (0.2 mg/L) + kin (0.5 mg/L) and BAP (0.5 mg/L) + kin (0.5 mg/L).
Original languageEnglish
Title of host publicationProceedings IS on Medicinal and Aromatic Plants – IMAPS 2010 and “History of Mayan Ethnopharmacology” – IMAPS 2011
EditorsJ. Ghaemghami
Pages143-152
Volume964
DOIs
Publication statusPublished - 2012
EventIS on Medicinal and Aromatic Plants – IMAPS 2010 and “History of Mayan Ethnopharmacology” – IMAPS 2011 -
Duration: 20 Nov 201123 Nov 2011

Conference

ConferenceIS on Medicinal and Aromatic Plants – IMAPS 2010 and “History of Mayan Ethnopharmacology” – IMAPS 2011
Period20/11/1123/11/11

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micropropagation
somatic embryogenesis
2,4-D
medicinal plants
explants
naphthaleneacetic acid
hypocotyls
cotyledons
Apiaceae
callus formation
plant growth substances
embryo (plant)
Ferula assa-foetida
leaves
flowers
seedlings

Cite this

Roozbeh, S., Otroshy, M., Bozorgipoor, R., Ebrahimi, M., Moeini Najafabadi, A., & Struik, P. C. (2012). Micropropagation of Ferula assa-foetida L. (a medicinal plant) via direct somatic embryogenesis. In J. Ghaemghami (Ed.), Proceedings IS on Medicinal and Aromatic Plants – IMAPS 2010 and “History of Mayan Ethnopharmacology” – IMAPS 2011 (Vol. 964, pp. 143-152) https://doi.org/10.17660/ActaHortic.2012.964.18
Roozbeh, S. ; Otroshy, M. ; Bozorgipoor, R. ; Ebrahimi, M. ; Moeini Najafabadi, A. ; Struik, P.C. / Micropropagation of Ferula assa-foetida L. (a medicinal plant) via direct somatic embryogenesis. Proceedings IS on Medicinal and Aromatic Plants – IMAPS 2010 and “History of Mayan Ethnopharmacology” – IMAPS 2011. editor / J. Ghaemghami. Vol. 964 2012. pp. 143-152
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title = "Micropropagation of Ferula assa-foetida L. (a medicinal plant) via direct somatic embryogenesis",
abstract = "Ferula assa-foetida is a medicinal plant from the Apiaceae family. It is a monocarpic plant and only flowers once during its growth period and then it dries forever and that is one of the main problems of multiplication. The experiment was carried out to examine the effect of explants, medium and plant growth regulators, on the in vitro direct somatic embryogenesis of asafetida (Ferula assa-foetida). Explants of root, hypocotyl and cotyledon (leaf) were investigated on MS and B5 medium combined with 2,4-D (0.2, 0.5 or 1 mg/L), BAP (0.2, 0.5 or 1 mg/L) and NAA (0.2, 0.5 or 1 mg/L), each of them was combined with kin (0, 0.2, 0.5 or 1 mg/L). The best direct somatic embryogenesis was obtained from explants of leaves, no significant effect between explants of cotyledon and hypocotyls was found. 2,4-D (0.2 mg/L) + kin (0.2 mg/L) resulted in the highest percent of direct somatic embryogenesis, also the maximum indirect somatic embryogenesis was obtained from explants of root grown on the 2,4-D (0.5 mg/L) + kin (0.2 mg/L). The highest value for direct somatic embryogenesis was found in MS medium combined with 2,4-D (0.2 mg/L) + kin (0.2 mg/L), while the maximum of indirect somatic embryogenesis was observed in B5 medium enriched with 2,4-D (0.5 mg/L) + kin (0.2 mg/L). The maximum percentage of embryo conversion into seedling was found with 2,4-D (0.5 mg/L) + kin (0.2 mg/L) in B5. Highest percent of callogenesis was observed on B5 containing NAA (1 mg/L) + kin (1 mg/L), BAP (0.2 mg/L) + kin (0.5 mg/L) and BAP (0.5 mg/L) + kin (0.5 mg/L).",
author = "S. Roozbeh and M. Otroshy and R. Bozorgipoor and M. Ebrahimi and {Moeini Najafabadi}, A. and P.C. Struik",
year = "2012",
doi = "10.17660/ActaHortic.2012.964.18",
language = "English",
volume = "964",
pages = "143--152",
editor = "J. Ghaemghami",
booktitle = "Proceedings IS on Medicinal and Aromatic Plants – IMAPS 2010 and “History of Mayan Ethnopharmacology” – IMAPS 2011",

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Roozbeh, S, Otroshy, M, Bozorgipoor, R, Ebrahimi, M, Moeini Najafabadi, A & Struik, PC 2012, Micropropagation of Ferula assa-foetida L. (a medicinal plant) via direct somatic embryogenesis. in J Ghaemghami (ed.), Proceedings IS on Medicinal and Aromatic Plants – IMAPS 2010 and “History of Mayan Ethnopharmacology” – IMAPS 2011. vol. 964, pp. 143-152, IS on Medicinal and Aromatic Plants – IMAPS 2010 and “History of Mayan Ethnopharmacology” – IMAPS 2011, 20/11/11. https://doi.org/10.17660/ActaHortic.2012.964.18

Micropropagation of Ferula assa-foetida L. (a medicinal plant) via direct somatic embryogenesis. / Roozbeh, S.; Otroshy, M.; Bozorgipoor, R.; Ebrahimi, M.; Moeini Najafabadi, A.; Struik, P.C.

Proceedings IS on Medicinal and Aromatic Plants – IMAPS 2010 and “History of Mayan Ethnopharmacology” – IMAPS 2011. ed. / J. Ghaemghami. Vol. 964 2012. p. 143-152.

Research output: Chapter in Book/Report/Conference proceedingConference paper

TY - GEN

T1 - Micropropagation of Ferula assa-foetida L. (a medicinal plant) via direct somatic embryogenesis

AU - Roozbeh, S.

AU - Otroshy, M.

AU - Bozorgipoor, R.

AU - Ebrahimi, M.

AU - Moeini Najafabadi, A.

AU - Struik, P.C.

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N2 - Ferula assa-foetida is a medicinal plant from the Apiaceae family. It is a monocarpic plant and only flowers once during its growth period and then it dries forever and that is one of the main problems of multiplication. The experiment was carried out to examine the effect of explants, medium and plant growth regulators, on the in vitro direct somatic embryogenesis of asafetida (Ferula assa-foetida). Explants of root, hypocotyl and cotyledon (leaf) were investigated on MS and B5 medium combined with 2,4-D (0.2, 0.5 or 1 mg/L), BAP (0.2, 0.5 or 1 mg/L) and NAA (0.2, 0.5 or 1 mg/L), each of them was combined with kin (0, 0.2, 0.5 or 1 mg/L). The best direct somatic embryogenesis was obtained from explants of leaves, no significant effect between explants of cotyledon and hypocotyls was found. 2,4-D (0.2 mg/L) + kin (0.2 mg/L) resulted in the highest percent of direct somatic embryogenesis, also the maximum indirect somatic embryogenesis was obtained from explants of root grown on the 2,4-D (0.5 mg/L) + kin (0.2 mg/L). The highest value for direct somatic embryogenesis was found in MS medium combined with 2,4-D (0.2 mg/L) + kin (0.2 mg/L), while the maximum of indirect somatic embryogenesis was observed in B5 medium enriched with 2,4-D (0.5 mg/L) + kin (0.2 mg/L). The maximum percentage of embryo conversion into seedling was found with 2,4-D (0.5 mg/L) + kin (0.2 mg/L) in B5. Highest percent of callogenesis was observed on B5 containing NAA (1 mg/L) + kin (1 mg/L), BAP (0.2 mg/L) + kin (0.5 mg/L) and BAP (0.5 mg/L) + kin (0.5 mg/L).

AB - Ferula assa-foetida is a medicinal plant from the Apiaceae family. It is a monocarpic plant and only flowers once during its growth period and then it dries forever and that is one of the main problems of multiplication. The experiment was carried out to examine the effect of explants, medium and plant growth regulators, on the in vitro direct somatic embryogenesis of asafetida (Ferula assa-foetida). Explants of root, hypocotyl and cotyledon (leaf) were investigated on MS and B5 medium combined with 2,4-D (0.2, 0.5 or 1 mg/L), BAP (0.2, 0.5 or 1 mg/L) and NAA (0.2, 0.5 or 1 mg/L), each of them was combined with kin (0, 0.2, 0.5 or 1 mg/L). The best direct somatic embryogenesis was obtained from explants of leaves, no significant effect between explants of cotyledon and hypocotyls was found. 2,4-D (0.2 mg/L) + kin (0.2 mg/L) resulted in the highest percent of direct somatic embryogenesis, also the maximum indirect somatic embryogenesis was obtained from explants of root grown on the 2,4-D (0.5 mg/L) + kin (0.2 mg/L). The highest value for direct somatic embryogenesis was found in MS medium combined with 2,4-D (0.2 mg/L) + kin (0.2 mg/L), while the maximum of indirect somatic embryogenesis was observed in B5 medium enriched with 2,4-D (0.5 mg/L) + kin (0.2 mg/L). The maximum percentage of embryo conversion into seedling was found with 2,4-D (0.5 mg/L) + kin (0.2 mg/L) in B5. Highest percent of callogenesis was observed on B5 containing NAA (1 mg/L) + kin (1 mg/L), BAP (0.2 mg/L) + kin (0.5 mg/L) and BAP (0.5 mg/L) + kin (0.5 mg/L).

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DO - 10.17660/ActaHortic.2012.964.18

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BT - Proceedings IS on Medicinal and Aromatic Plants – IMAPS 2010 and “History of Mayan Ethnopharmacology” – IMAPS 2011

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Roozbeh S, Otroshy M, Bozorgipoor R, Ebrahimi M, Moeini Najafabadi A, Struik PC. Micropropagation of Ferula assa-foetida L. (a medicinal plant) via direct somatic embryogenesis. In Ghaemghami J, editor, Proceedings IS on Medicinal and Aromatic Plants – IMAPS 2010 and “History of Mayan Ethnopharmacology” – IMAPS 2011. Vol. 964. 2012. p. 143-152 https://doi.org/10.17660/ActaHortic.2012.964.18