Alstroemeria rhizomes were micropropagated on semi-solid medium (AM) and in liquid medium (LM). In LM, growth was much enhanced (ca. 70%). Adequate gas exchange was crucial. This was obtained by agitation and in static medium by a sufficient large contact area of the explant and the gaseous atmosphere of the headspace. The medium became rapidly depleted with respect to both organic and inorganic nutrients. Manual addition of extra medium ingredients during the culture enhanced growth. Extra nutrients were also successfully administered via slow-release tools. Two types of slow-release tools were used, Osmocote for inorganic nutrients and newly developed castings for sucrose. Addition of extra 6-benzylaminopurine (BAP) during the culture stimulated the outgrowth of lateral rhizomes. The extra BAP could be included in the slow-release castings for sucrose.
|Journal||Propagation of ornamental plants|
|Publication status||Published - 2010|
- mass propagation
- gelling agent