Microcalorimetric study on the influence of temperature on bacterial coaggregation

F. Postcollec, W. Norde, H.C. van der Mei, H.J. Busscher

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5 Citations (Scopus)

Abstract

Binding isotherms and heats of interaction have been determined at 15, 25, and 40°C for a coaggregating and a non-coaggregating oral bacterial pair. Heats of interaction were measured upon three consecutive injections of streptococci into an actinomyces suspension using isothermal titration calorimetry. After each injection, the number of streptococci injected remaining free in suspension was quantified microscopically and the degree of binding between the two bacterial strains was established. The coaggregating pair shows positive cooperative binding. The highest cooperativity, at 25°C, correlates with a strong, macroscopically visible coaggregation. The non-coaggregating pair shows low cooperativity and lacks macroscopically visible coaggregation. Interactions between the coaggregating partners seem to be mainly due to specific, enthalpically saturable and favorable binding sites. Even though the enthalpic part of the interaction is saturated, cooperativity increases with consecutive injections, implying that the coaggregation phenomenon is driven by entropy gain. The change in heat capacity (¿Cp) is positive for the non-coaggregating pair from 15-40°C as well as for the coaggregating pair beyond 25°C. At lower temperatures the coaggregating pair causes a negative ¿Cp. The decrease in heat capacity together with an increase in entropy is considered to be indicative of hydrophobic interactions playing an important role in the formation of large coaggregates as observed for the coaggregating pair at 25°C.
Original languageEnglish
Pages (from-to)461-467
JournalJournal of Colloid and Interface Science
Volume287
Issue number2
DOIs
Publication statusPublished - 2005

Keywords

  • streptococcus-sanguis
  • actinomyces-viscosus
  • oral streptococci
  • heat-capacity
  • binding
  • saliva
  • hydroxylapatite
  • adherence
  • adhesion
  • pairs

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