Microbial enzymes mined from the Urania deep-sea hypersaline anoxic basin

Manuel Ferrer*, Olga V. Golyshina, Tatyana N. Chernikova, Amit N. Khachane, Vitor A.P. Martins Dos Santos, Michail M. Yakimov, Kenneth N. Timmis, Peter N. Golyshin

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

125 Citations (Scopus)

Abstract

We created a metagenome expression library from the brine:seawater interface of the Urania hypersaline basin, screened it for esterases, and characterized five of these. Two had no significant sequence homology to known esterases, hydrolyzed both carboxylesters and thioesters, and exhibited unusual, habitat-specific characteristics (preference for high hydrostatic pressure and salinity). One has an unusual structural signature incorporating three catalytic active centers mediating distinct hydrolytic activities and an adaptive tertiary-quaternary structure that alters between three molecular states, according to the prevailing physicochemical conditions. Some of the esterases have high activities, specificities, enantioselectivities, and exceptional stability in polar solvents, and they are therefore potentially useful for industrial biotransformations. One possesses the highest enantioselectivity toward an ester of the important chiral synthon solketal (E: 126[S]; 98%ee).

Original languageEnglish
Pages (from-to)895-904
Number of pages10
JournalChemistry and Biology
Volume12
Issue number8
DOIs
Publication statusPublished - Aug 2005
Externally publishedYes

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