Microbial diversity, nitrogen loss and grass production after addition of Effective Micro-organisms® (EM) to slurry manure

P.C.J. van Vliet, J. Bloem, R.G.M. de Goede

Research output: Contribution to journalArticleAcademicpeer-review

41 Citations (Scopus)

Abstract

In this study, we used a DNA fingerprinting technique (PCR-DGGE) to investigate if bacteria present in an activated EM suspension (EM-A) were able to maintain or reach significant relative abundances after addition to slurry manure. We also investigated effects of the addition of EM-A to slurry manure on nitrogen losses from the manure and grass biomass production after application of the manure as organic fertilizer. EM® and Agri-mest® were tested in a factorial set-up so that the factors could be distinguished. Effective Organisms (EM) is a mixture of several effective, disease-depressing micro-organisms. Agri-mest contains energized minerals, which will influence biochemical processes occurring in the manure. Different EM-stocks, and EM-Active suspensions prepared by different users showed large variation in bacterial community structure and, thus, low reproducibility. Through the addition of Agri-mest to the slurry manure, loss of inorganic nitrogen from the slurry manure appeared to be reduced. Moreover, after application of such Agri-mest slurry as an organic fertilizer, nitrogen uptake by grass was reduced. Addition of EM-A had no measurable effects on the bacterial diversity and the chemical composition of the slurry manure. In a pot experiment, also no effects of EM on nitrogen uptake and grass biomass production were recorded.
Original languageEnglish
Pages (from-to)188-198
JournalApplied Soil Ecology
Volume32
Issue number2
DOIs
Publication statusPublished - 2006

Keywords

  • gel-electrophoresis analysis
  • 16s ribosomal-rna
  • bacteria
  • soil
  • communities
  • survival

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