Microarray challenges in ecology

J.E. Kammenga, M.A. Herman, N.J. Ouborg, L. Johnson, R. Breitling

Research output: Contribution to journalArticleAcademicpeer-review

62 Citations (Scopus)

Abstract

Microarrays are used to measure simultaneously the amount of mRNAs transcribed from many genes. They were originally designed for gene expression profiling in relatively simple biological systems, such as cell lines and model systems under constant laboratory conditions. This poses a challenge to ecologists who increasingly want to use microarrays to unravel the genetic mechanisms underlying complex interactions among organisms and between organisms and their environment. Here, we discuss typical experimental and statistical problems that arise when analyzing genome-wide expression profiles in an ecological context. We show that experimental design and environmental confounders greatly influence the identification of candidate genes in ecological microarray studies, and that following several simple recommendations could facilitate the analysis of microarray data in ecological settings.
Original languageEnglish
Pages (from-to)273-279
JournalTrends in Ecology and Evolution
Volume22
Issue number5
DOIs
Publication statusPublished - 2007

Keywords

  • gene-expression profiles
  • drosophila-melanogaster
  • ectomycorrhizal fungus
  • nicotiana-attenuata
  • natural-populations
  • cdna microarrays
  • evolution
  • arabidopsis
  • genomics
  • patterns

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