Microarray analysis is widely used to identify transcriptional changes associated with genetic perturbation or signaling events. Here we describe its application in the identification of plant transcription factor target genes with emphasis on the design of suitable DNA constructs for controlling TF activity, the experimental setup, the statistical analysis of the microarray data, and the validation of target genes.
|Title of host publication||Plant Transcription Factors: Methods and Protocols.|
|Editors||L. Yuan, S.E. Perry|
|Number of pages||347|
|Publication status||Published - 2011|
|Name||Methods in Molecular Biology|