Microarray-Based Identification of Transcription Factor Target Genes

M. Gorte, A. Horstman, R.B. Page, R. Heidstra, A. Stromberg, K.A. Boutilier

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

4 Citations (Scopus)

Abstract

Microarray analysis is widely used to identify transcriptional changes associated with genetic perturbation or signaling events. Here we describe its application in the identification of plant transcription factor target genes with emphasis on the design of suitable DNA constructs for controlling TF activity, the experimental setup, the statistical analysis of the microarray data, and the validation of target genes.
Original languageEnglish
Title of host publicationPlant Transcription Factors: Methods and Protocols.
EditorsL. Yuan, S.E. Perry
Pages119-141
Number of pages347
DOIs
Publication statusPublished - 2011

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Number754

Keywords

  • amplification efficiency
  • cycloheximide
  • endogenous reference gene
  • inducible gene expression systems
  • Microarray
  • quantitative real-time RT-PCR
  • relative quantification
  • target gene
  • transcription factor

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