Microarray-based Detection of Antibiotic Resisteance Genes in Salmonella

A.H.A.M. van Hoek, H.J.M. Aarts

    Research output: Contribution to journalArticleAcademicpeer-review

    Abstract

    In the presented study, 143 Salmonella isolates belonging to 26 different serovars were screened for the presence of antibiotic resistance genes by microarray analysis. The microarray contained a total of 223 oligonucleotides representing genes encoding for resistance to the following antibiotic classes: aminoglycoside, ß-lactam, chloramphenicol, MLS, sulfonamide, tetracycline, trimethoprim, and vancomycin. To a large extent, the microarray data were consistent to the general findings concerning antibiotic resistance in Salmonella. Most of the analyzed isolates, harbored three or more resistance genes with the highest numbers found in isolates belonging to the Salmonella serovars Typhimurium, Paratyphi B var. Java, Bredeney, Saint Paul and Heidelberg and the only Give isolate investigated
    Original languageEnglish
    Pages (from-to)95-108
    JournalFood Analytical Methods
    Volume1
    Issue number2
    DOIs
    Publication statusPublished - 2008

    Keywords

    • spectrum beta-lactamases
    • typhimurium dt104
    • drug-resistance
    • mar locus
    • integrons
    • bacteria
    • animals
    • humans
    • susceptibility
    • netherlands

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