Microarray-based Detection of Antibiotic Resisteance Genes in Salmonella

A.H.A.M. van Hoek; H.J.M. Aarts

doi:10.1007/s12161-007-9012-1

Microarray-based Detection of Antibiotic Resisteance Genes in Salmonella

A.H.A.M. van Hoek, H.J.M. Aarts

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

In the presented study, 143 Salmonella isolates belonging to 26 different serovars were screened for the presence of antibiotic resistance genes by microarray analysis. The microarray contained a total of 223 oligonucleotides representing genes encoding for resistance to the following antibiotic classes: aminoglycoside, ß-lactam, chloramphenicol, MLS, sulfonamide, tetracycline, trimethoprim, and vancomycin. To a large extent, the microarray data were consistent to the general findings concerning antibiotic resistance in Salmonella. Most of the analyzed isolates, harbored three or more resistance genes with the highest numbers found in isolates belonging to the Salmonella serovars Typhimurium, Paratyphi B var. Java, Bredeney, Saint Paul and Heidelberg and the only Give isolate investigated

Original language	English
Pages (from-to)	95-108
Journal	Food Analytical Methods
Volume	1
Issue number	2
DOIs	https://doi.org/10.1007/s12161-007-9012-1
Publication status	Published - 2008

Keywords

spectrum beta-lactamases
typhimurium dt104
drug-resistance
mar locus
integrons
bacteria
animals
humans
susceptibility
netherlands

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title = "Microarray-based Detection of Antibiotic Resisteance Genes in Salmonella",

abstract = "In the presented study, 143 Salmonella isolates belonging to 26 different serovars were screened for the presence of antibiotic resistance genes by microarray analysis. The microarray contained a total of 223 oligonucleotides representing genes encoding for resistance to the following antibiotic classes: aminoglycoside, {\ss}-lactam, chloramphenicol, MLS, sulfonamide, tetracycline, trimethoprim, and vancomycin. To a large extent, the microarray data were consistent to the general findings concerning antibiotic resistance in Salmonella. Most of the analyzed isolates, harbored three or more resistance genes with the highest numbers found in isolates belonging to the Salmonella serovars Typhimurium, Paratyphi B var. Java, Bredeney, Saint Paul and Heidelberg and the only Give isolate investigated",

keywords = "spectrum beta-lactamases, typhimurium dt104, drug-resistance, mar locus, integrons, bacteria, animals, humans, susceptibility, netherlands",

author = "{van Hoek}, A.H.A.M. and H.J.M. Aarts",

year = "2008",

doi = "10.1007/s12161-007-9012-1",

language = "English",

volume = "1",

pages = "95--108",

journal = "Food Analytical Methods",

issn = "1936-9751",

publisher = "Springer",

number = "2",

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TY - JOUR

T1 - Microarray-based Detection of Antibiotic Resisteance Genes in Salmonella

AU - van Hoek, A.H.A.M.

AU - Aarts, H.J.M.

PY - 2008

Y1 - 2008

N2 - In the presented study, 143 Salmonella isolates belonging to 26 different serovars were screened for the presence of antibiotic resistance genes by microarray analysis. The microarray contained a total of 223 oligonucleotides representing genes encoding for resistance to the following antibiotic classes: aminoglycoside, ß-lactam, chloramphenicol, MLS, sulfonamide, tetracycline, trimethoprim, and vancomycin. To a large extent, the microarray data were consistent to the general findings concerning antibiotic resistance in Salmonella. Most of the analyzed isolates, harbored three or more resistance genes with the highest numbers found in isolates belonging to the Salmonella serovars Typhimurium, Paratyphi B var. Java, Bredeney, Saint Paul and Heidelberg and the only Give isolate investigated

AB - In the presented study, 143 Salmonella isolates belonging to 26 different serovars were screened for the presence of antibiotic resistance genes by microarray analysis. The microarray contained a total of 223 oligonucleotides representing genes encoding for resistance to the following antibiotic classes: aminoglycoside, ß-lactam, chloramphenicol, MLS, sulfonamide, tetracycline, trimethoprim, and vancomycin. To a large extent, the microarray data were consistent to the general findings concerning antibiotic resistance in Salmonella. Most of the analyzed isolates, harbored three or more resistance genes with the highest numbers found in isolates belonging to the Salmonella serovars Typhimurium, Paratyphi B var. Java, Bredeney, Saint Paul and Heidelberg and the only Give isolate investigated

KW - spectrum beta-lactamases

KW - typhimurium dt104

KW - drug-resistance

KW - mar locus

KW - integrons

KW - bacteria

KW - animals

KW - humans

KW - susceptibility

KW - netherlands

U2 - 10.1007/s12161-007-9012-1

DO - 10.1007/s12161-007-9012-1

M3 - Article

SN - 1936-9751

VL - 1

SP - 95

EP - 108

JO - Food Analytical Methods

JF - Food Analytical Methods

IS - 2

ER -

Microarray-based Detection of Antibiotic Resisteance Genes in Salmonella

Abstract

Keywords

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