Microalgae growth on concentrated human urine

Research output: Contribution to journalArticleAcademicpeer-review

23 Citations (Scopus)

Abstract

In this study, for the first time, a microalga was grown on non-diluted human urine. The essential growth requirements for the species Chlorella sorokiniana were determined for different types of human urine (fresh, hydrolysed, male and female). Batch experimental results using microtiter plates showed that both fresh and synthetic urine supported rapid growth of this species, provided additional trace elements (Cu, Fe, Mn, and Zn) were added. When using hydrolysed urine instead of fresh urine, additional magnesium had to be added as it precipitates during hydrolysis of urea. C. sorokiniana was able to grow on non-diluted urine with a specific growth rate as high as 0.104 h-1 under light-limited conditions (105 µmol photons m-2 s-1), and the growth was not inhibited by ammonium up to a concentration of 1,400 mg NH4+-N L-1. The highest growth rate on human urine was as high as 0.158 h-1. Because it was demonstrated that concentrated urine is a rich and good nutrient source for the production of microalgae, its application for a large-scale economical and sustainable microalgae production for biochemicals, biofuels and biofertilizers becomes feasible.
Original languageEnglish
Pages (from-to)287-297
JournalJournal of Applied Phycology
Volume26
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

microalgae
urine
Chlorella sorokiniana
biofertilizer
microalga
biofertilizers
biofuels
biofuel
specific growth rate
urea
trace elements
hydrolysis
magnesium
ammonium
trace element
nutrient
nutrients

Keywords

  • separated domestic waste(water)
  • chlorella-sorokiniana
  • struvite precipitation
  • urea hydrolysis
  • recovery
  • photobioreactor
  • nutrients
  • cultures
  • energy
  • algae

Cite this

@article{7726dfb54c0d459eb5838b575418ca92,
title = "Microalgae growth on concentrated human urine",
abstract = "In this study, for the first time, a microalga was grown on non-diluted human urine. The essential growth requirements for the species Chlorella sorokiniana were determined for different types of human urine (fresh, hydrolysed, male and female). Batch experimental results using microtiter plates showed that both fresh and synthetic urine supported rapid growth of this species, provided additional trace elements (Cu, Fe, Mn, and Zn) were added. When using hydrolysed urine instead of fresh urine, additional magnesium had to be added as it precipitates during hydrolysis of urea. C. sorokiniana was able to grow on non-diluted urine with a specific growth rate as high as 0.104 h-1 under light-limited conditions (105 µmol photons m-2 s-1), and the growth was not inhibited by ammonium up to a concentration of 1,400 mg NH4+-N L-1. The highest growth rate on human urine was as high as 0.158 h-1. Because it was demonstrated that concentrated urine is a rich and good nutrient source for the production of microalgae, its application for a large-scale economical and sustainable microalgae production for biochemicals, biofuels and biofertilizers becomes feasible.",
keywords = "separated domestic waste(water), chlorella-sorokiniana, struvite precipitation, urea hydrolysis, recovery, photobioreactor, nutrients, cultures, energy, algae",
author = "K. Tuantet and M.G.J. Janssen and H. Temmink and G. Zeeman and R.H. Wijffels and C.J.N. Buisman",
year = "2014",
doi = "10.1007/s10811-013-0108-2",
language = "English",
volume = "26",
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Microalgae growth on concentrated human urine. / Tuantet, K.; Janssen, M.G.J.; Temmink, H.; Zeeman, G.; Wijffels, R.H.; Buisman, C.J.N.

In: Journal of Applied Phycology, Vol. 26, No. 1, 2014, p. 287-297.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Microalgae growth on concentrated human urine

AU - Tuantet, K.

AU - Janssen, M.G.J.

AU - Temmink, H.

AU - Zeeman, G.

AU - Wijffels, R.H.

AU - Buisman, C.J.N.

PY - 2014

Y1 - 2014

N2 - In this study, for the first time, a microalga was grown on non-diluted human urine. The essential growth requirements for the species Chlorella sorokiniana were determined for different types of human urine (fresh, hydrolysed, male and female). Batch experimental results using microtiter plates showed that both fresh and synthetic urine supported rapid growth of this species, provided additional trace elements (Cu, Fe, Mn, and Zn) were added. When using hydrolysed urine instead of fresh urine, additional magnesium had to be added as it precipitates during hydrolysis of urea. C. sorokiniana was able to grow on non-diluted urine with a specific growth rate as high as 0.104 h-1 under light-limited conditions (105 µmol photons m-2 s-1), and the growth was not inhibited by ammonium up to a concentration of 1,400 mg NH4+-N L-1. The highest growth rate on human urine was as high as 0.158 h-1. Because it was demonstrated that concentrated urine is a rich and good nutrient source for the production of microalgae, its application for a large-scale economical and sustainable microalgae production for biochemicals, biofuels and biofertilizers becomes feasible.

AB - In this study, for the first time, a microalga was grown on non-diluted human urine. The essential growth requirements for the species Chlorella sorokiniana were determined for different types of human urine (fresh, hydrolysed, male and female). Batch experimental results using microtiter plates showed that both fresh and synthetic urine supported rapid growth of this species, provided additional trace elements (Cu, Fe, Mn, and Zn) were added. When using hydrolysed urine instead of fresh urine, additional magnesium had to be added as it precipitates during hydrolysis of urea. C. sorokiniana was able to grow on non-diluted urine with a specific growth rate as high as 0.104 h-1 under light-limited conditions (105 µmol photons m-2 s-1), and the growth was not inhibited by ammonium up to a concentration of 1,400 mg NH4+-N L-1. The highest growth rate on human urine was as high as 0.158 h-1. Because it was demonstrated that concentrated urine is a rich and good nutrient source for the production of microalgae, its application for a large-scale economical and sustainable microalgae production for biochemicals, biofuels and biofertilizers becomes feasible.

KW - separated domestic waste(water)

KW - chlorella-sorokiniana

KW - struvite precipitation

KW - urea hydrolysis

KW - recovery

KW - photobioreactor

KW - nutrients

KW - cultures

KW - energy

KW - algae

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