MIBiG 2.0: a repository for biosynthetic gene clusters of known functions

S.A. Kautsar, Kai Blin, Simon Shaw, J.C. Navarro Munoz, Barbara Terlouw, J.J.J. van der Hooft, Jeffrey A. Van Santen, V. Tracanna, Hernando Suarez Duran, V. Pascal Andreu, Nelly Selem Mojica, Mohammad Alanjary, Serina Robinson, George Lund, Samuel C. Epstein, Ashley C. Sisto, Louise K. Charkoudian, Jérôme Collemare, Roger G. Linington, Tilmann WeberM.H. Medema*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

421 Citations (Scopus)

Abstract

Fueled by the explosion of (meta)genomic data, genome mining of specialized metabolites has become a major technology for drug discovery and studying microbiome ecology. In these efforts, computational tools like antiSMASH have played a central role through the analysis of Biosynthetic Gene Clusters (BGCs). Thousands of candidate BGCs from microbial genomes have been identified and stored in public databases. Interpreting the function and novelty of these predicted BGCs requires comparison with a well-documented set of BGCs of known function. The MIBiG (Minimum Information about a Biosynthetic Gene Cluster) Data Standard and Repository was established in 2015 to enable curation and storage of known BGCs. Here, we present MIBiG 2.0, which encompasses major updates to the schema, the data, and the online repository itself. Over the past five years, 851 new BGCs have been added. Additionally, we performed extensive manual data curation of all entries to improve the annotation quality of our repository. We also redesigned the data schema to ensure the compliance of future annotations. Finally, we improved the user experience by adding new features such as query searches and a statistics page, and enabled direct link-outs to chemical structure databases. The repository is accessible online at https://mibig.secondarymetabolites.org/.
Original languageEnglish
Pages (from-to)D454-D458
Number of pages4
JournalNucleic acids research
Volume48
Issue numberD1
Early online date15 Oct 2019
DOIs
Publication statusPublished - 8 Jan 2020

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