TY - CHAP
T1 - Methods for elucidating elemental distribution in hyperaccumulator plants
AU - van der Ent, A.
AU - Purwadi, Imam
AU - Harris, Hugh H.
AU - Kopittke, Peter M.
AU - Przybyłowicz, Wojciech J.
AU - Mesjasz-Przybyłowicz, Jolanta
PY - 2021
Y1 - 2021
N2 - Crucial to many investigations on the ecophysiology of hyperaccumulator plants is visualization of the spatial distribution of metal(loid)s in their tissues. A wide variety of methods can be used to obtain information on the distribution of elements in plant tissues, and X-ray elemental mapping techniques are especially widely used. Microanalytical investigations place strict demands on sample collection, preparation, and analytical conditions, in order to avoid elemental redistribution, chemical modification, or ultrastructural alterations. This chapter summarizes a range of techniques that can be used to visualise the elemental distribution in hyperaccumulator plants, including synchrotron and laboratory-based X-ray fluorescence microscopy (micro-XRF), proton-induced X-ray emission (PIXE), scanning/transmission electron microscopy with energy-dispersive X-ray spectroscopy (SEM/TEM-EDS), laser ablation-inductively-coupled plasma—mass spectrometry (LA-ICP-MS), nanoscale secondary ion mass spectroscopy (NanoSIMS), autoradiography, histochemical methods, and confocal microscopy using fluorophores.
AB - Crucial to many investigations on the ecophysiology of hyperaccumulator plants is visualization of the spatial distribution of metal(loid)s in their tissues. A wide variety of methods can be used to obtain information on the distribution of elements in plant tissues, and X-ray elemental mapping techniques are especially widely used. Microanalytical investigations place strict demands on sample collection, preparation, and analytical conditions, in order to avoid elemental redistribution, chemical modification, or ultrastructural alterations. This chapter summarizes a range of techniques that can be used to visualise the elemental distribution in hyperaccumulator plants, including synchrotron and laboratory-based X-ray fluorescence microscopy (micro-XRF), proton-induced X-ray emission (PIXE), scanning/transmission electron microscopy with energy-dispersive X-ray spectroscopy (SEM/TEM-EDS), laser ablation-inductively-coupled plasma—mass spectrometry (LA-ICP-MS), nanoscale secondary ion mass spectroscopy (NanoSIMS), autoradiography, histochemical methods, and confocal microscopy using fluorophores.
U2 - 10.1007/978-3-030-58904-2_10
DO - 10.1007/978-3-030-58904-2_10
M3 - Chapter
SN - 9783030589035
T3 - Mineral Resource Reviews series
SP - 197
EP - 214
BT - Agromining: Farming for Metals
A2 - van der Ent, Antony
A2 - Baker, Alan J. M.
A2 - Echevarria, Guillaume
A2 - Simonnot, Marie-Odile
A2 - Morel, Jean Louis
PB - Springer
CY - Cham
ER -