Methods for detecting cyst nematodes

J. Helder; G. Karssen; S.J.J. van den Elsen; M.H.M. Holterman; P.J.W. Mooijman; R.V. Staps; R. Landeweert; H. Hekman; J. Bakker

Methods for detecting cyst nematodes

J. Helder (Inventor), G. Karssen (Inventor), S.J.J. van den Elsen (Inventor), M.H.M. Holterman (Inventor), P.J.W. Mooijman (Inventor), R.V. Staps (Inventor), R. Landeweert (Inventor), H. Hekman (Inventor), J. Bakker (Inventor)

Research output: Patent

Abstract

The present invention relates to a method for determining the presence of a cyst nematode in a sample comprising the steps of : providing a pair of bidirectional oligonucleotide primers or an oligonucleotide probe that hybridizes specifically, under stringent hybridization conditions, to a nucleic acid sequence encoding the SSU rRNA or LSU rRNA, or the complement or transcript thereof, of a sub genus-cluster of nematodes, said subgenus-cluster comprising cyst nematodes belonging to at least one species of nematode, and wherein said primers or probe do not hybridize to a nucleic acid sequence encoding the LSU rRNA, or the complement or transcript thereof, of cyst nematodes not part of said subgenus-cluster of cyst nematodes; providing a sample in which the presence of the cyst nematode is to be detected, and performing a nucleic acid detection assay on said sample using said pair of bidirectional oligonucleotide primers or said oligonucleotide probe.

Original language	English
Patent number	WO2008079012
Priority date	27/12/06
Publication status	Published - 3 Jul 2008

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title = "Methods for detecting cyst nematodes",

abstract = "The present invention relates to a method for determining the presence of a cyst nematode in a sample comprising the steps of : providing a pair of bidirectional oligonucleotide primers or an oligonucleotide probe that hybridizes specifically, under stringent hybridization conditions, to a nucleic acid sequence encoding the SSU rRNA or LSU rRNA, or the complement or transcript thereof, of a sub genus-cluster of nematodes, said subgenus-cluster comprising cyst nematodes belonging to at least one species of nematode, and wherein said primers or probe do not hybridize to a nucleic acid sequence encoding the LSU rRNA, or the complement or transcript thereof, of cyst nematodes not part of said subgenus-cluster of cyst nematodes; providing a sample in which the presence of the cyst nematode is to be detected, and performing a nucleic acid detection assay on said sample using said pair of bidirectional oligonucleotide primers or said oligonucleotide probe.",

author = "J. Helder and G. Karssen and {van den Elsen}, S.J.J. and M.H.M. Holterman and P.J.W. Mooijman and R.V. Staps and R. Landeweert and H. Hekman and J. Bakker",

year = "2008",

month = jul,

day = "3",

language = "English",

type = "Patent",

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TY - PAT

T1 - Methods for detecting cyst nematodes

AU - Helder, J.

AU - Karssen, G.

AU - van den Elsen, S.J.J.

AU - Holterman, M.H.M.

AU - Mooijman, P.J.W.

AU - Staps, R.V.

AU - Landeweert, R.

AU - Hekman, H.

AU - Bakker, J.

PY - 2008/7/3

Y1 - 2008/7/3

N2 - The present invention relates to a method for determining the presence of a cyst nematode in a sample comprising the steps of : providing a pair of bidirectional oligonucleotide primers or an oligonucleotide probe that hybridizes specifically, under stringent hybridization conditions, to a nucleic acid sequence encoding the SSU rRNA or LSU rRNA, or the complement or transcript thereof, of a sub genus-cluster of nematodes, said subgenus-cluster comprising cyst nematodes belonging to at least one species of nematode, and wherein said primers or probe do not hybridize to a nucleic acid sequence encoding the LSU rRNA, or the complement or transcript thereof, of cyst nematodes not part of said subgenus-cluster of cyst nematodes; providing a sample in which the presence of the cyst nematode is to be detected, and performing a nucleic acid detection assay on said sample using said pair of bidirectional oligonucleotide primers or said oligonucleotide probe.

AB - The present invention relates to a method for determining the presence of a cyst nematode in a sample comprising the steps of : providing a pair of bidirectional oligonucleotide primers or an oligonucleotide probe that hybridizes specifically, under stringent hybridization conditions, to a nucleic acid sequence encoding the SSU rRNA or LSU rRNA, or the complement or transcript thereof, of a sub genus-cluster of nematodes, said subgenus-cluster comprising cyst nematodes belonging to at least one species of nematode, and wherein said primers or probe do not hybridize to a nucleic acid sequence encoding the LSU rRNA, or the complement or transcript thereof, of cyst nematodes not part of said subgenus-cluster of cyst nematodes; providing a sample in which the presence of the cyst nematode is to be detected, and performing a nucleic acid detection assay on said sample using said pair of bidirectional oligonucleotide primers or said oligonucleotide probe.

M3 - Patent

M1 - WO2008079012

ER -

Methods for detecting cyst nematodes

Abstract

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