Methods for detecting cyst nematodes

J. Helder (Inventor), G. Karssen (Inventor), S.J.J. van den Elsen (Inventor), M.H.M. Holterman (Inventor), P.J.W. Mooijman (Inventor), R.V. Staps (Inventor), R. Landeweert (Inventor), H. Hekman (Inventor), J. Bakker (Inventor)

Research output: PatentOther research output

Abstract

The present invention relates to a method for determining the presence of a cyst nematode in a sample comprising the steps of : providing a pair of bidirectional oligonucleotide primers or an oligonucleotide probe that hybridizes specifically, under stringent hybridization conditions, to a nucleic acid sequence encoding the SSU rRNA or LSU rRNA, or the complement or transcript thereof, of a sub genus-cluster of nematodes, said subgenus-cluster comprising cyst nematodes belonging to at least one species of nematode, and wherein said primers or probe do not hybridize to a nucleic acid sequence encoding the LSU rRNA, or the complement or transcript thereof, of cyst nematodes not part of said subgenus-cluster of cyst nematodes; providing a sample in which the presence of the cyst nematode is to be detected, and performing a nucleic acid detection assay on said sample using said pair of bidirectional oligonucleotide primers or said oligonucleotide probe.
Original languageEnglish
Patent numberWO2008079012
Priority date27/12/06
Publication statusPublished - 3 Jul 2008

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