Methodology for health risk assessment of combined exposures to multiple chemicals

Anna Beronius*, Johanna Zilliacus, Annika Hanberg, Mirjam Luijten, Hilko van der Voet, Jacob van Klaveren

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)


Focus on risks to human health and the environment from combined exposure to multiple chemicals (“mixture risk assessment”) has increased in the last couple of decades. There has been a rise in awareness and concern in the community, especially concerning unintentional environmental exposure to unknown chemical mixtures. The Horizon 2020 project EuroMix has developed methodologies and tools for mixture risk assessment with a focus on component-based approach where substances are grouped based on toxicological considerations. Dose addition is used as the model for calculating the combined toxicity of mixture components. The methodology is anchored in the Adverse Outcome Pathway (AOP) concept, which provides a structured basis for e.g. grouping substances into assessment groups and identifying and collecting relevant toxicity data. The aim of this paper is to describes development of the methodology for mixture risk assessment and to provide detailed methodology for problem formulation, use of AOP networks for development of tiered testing strategies and grouping of substances, as well as considerations for use of dose addition methodology.

Original languageEnglish
Article number111520
JournalFood and Chemical Toxicology
Publication statusPublished - Sep 2020


  • Adverse outcome pathway
  • Combined exposure
  • Mixture
  • Risk assessment

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