Methodology development for partial resistance testing of potato cultivars resistant to M. chitwoodi

M.G. Teklu, T.H. Been, C.H. Schomaker

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

Abstract

M. chitwoodi was first described in 1995 in The Netherlands (Karssen, 1995). It is now listed as a quarantine organism in the EPPO region with 4 EU member states officially infested. Since 1996, research has been initiated to identify resistant genes against M. chitwoodi from wild species of tuber bearing potatoes and integrate these genes into cultivated potatoes. Currently, several breeding companies successfully produced resistant genotypes against M. chitwoodi. Parallel to this, research was started to develop a standard methodology to screen the partial resistance of these genotypes. Population dynamical models were used to estimate their level of resistance, expressed as percentage of relative susceptibility (rs). This methodology provides farmers with quantitative information on the effect of growing resistant potatoes at any initial population density in their field. The models were first tested in a pilot project in 2010 with 3 resistant potato genotypes in (5 kg) pots at a range of 13 nematode densities. In 2011, another 8 genotypes were tested in (10 kg) pots at a range of 12 densities. The results showed that Seinhorst’s population dynamical models for nematodes with multiple generations fitted well, except in one genotype tested in 2011which lacked resistance to M. chitwoodi, and a reduction of the number of densities used seems possible. In 2012, research was initiated to investigate whether the pot size can be downscaled from 10 to 5 or even to 2 kg pots – also at 12 densities -, without loss of quality of the estimated relative susceptibility. Also, growth, yield loss and quality damage as root knot index (RKI) were assessed and compared. Genotypes 2011M1, MDG2 and cv. Désirée (control) were the tested potatoes. The population dynamical model fitted well for the genotypes tested. The maximum multiplications rate “a” and the maximum population density “M” at 2, 5 and 10 kg pots were estimated and used to calculate the rsa and rsM values . Despite a decrease in “a” and “M” values with increasing pot size the rsa values were relatively stable. The rsM values were a bit higher in 5 kg pots. Seinhorst yield models used to describe the fresh tuber weight also fitted well. The RKI values obtained from the three pots sizes were also stable as a quality measure for industrial processing. Overall results indicate the possibility of downscaling the resistance test for M. chitwoodi in potato in terms of pot size and number of densities. Implications of the current research in the development of a cheap and reliable resistance test will be discussed.
Original languageEnglish
Title of host publicationProceedings of the 52nd Annual meeting of Society of nematologist, July 14- 17, 2013, Knoxville, Tennessee, USA
Place of PublicationKnoxville, Tennessee, USA
Pages106-107
Publication statusPublished - 2013
Event52nd Annual meeting of Society of nematologist, Knoxville, Tennessee, USA -
Duration: 14 Jul 201317 Jul 2013

Conference

Conference52nd Annual meeting of Society of nematologist, Knoxville, Tennessee, USA
Period14/07/1317/07/13

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potatoes
genotype
cultivars
testing
knots
methodology
tubers
population density
Nematoda
quarantine
research and development
processing technology
Netherlands
genes
farmers
organisms
breeding

Cite this

Teklu, M. G., Been, T. H., & Schomaker, C. H. (2013). Methodology development for partial resistance testing of potato cultivars resistant to M. chitwoodi. In Proceedings of the 52nd Annual meeting of Society of nematologist, July 14- 17, 2013, Knoxville, Tennessee, USA (pp. 106-107). Knoxville, Tennessee, USA.
Teklu, M.G. ; Been, T.H. ; Schomaker, C.H. / Methodology development for partial resistance testing of potato cultivars resistant to M. chitwoodi. Proceedings of the 52nd Annual meeting of Society of nematologist, July 14- 17, 2013, Knoxville, Tennessee, USA. Knoxville, Tennessee, USA, 2013. pp. 106-107
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abstract = "M. chitwoodi was first described in 1995 in The Netherlands (Karssen, 1995). It is now listed as a quarantine organism in the EPPO region with 4 EU member states officially infested. Since 1996, research has been initiated to identify resistant genes against M. chitwoodi from wild species of tuber bearing potatoes and integrate these genes into cultivated potatoes. Currently, several breeding companies successfully produced resistant genotypes against M. chitwoodi. Parallel to this, research was started to develop a standard methodology to screen the partial resistance of these genotypes. Population dynamical models were used to estimate their level of resistance, expressed as percentage of relative susceptibility (rs). This methodology provides farmers with quantitative information on the effect of growing resistant potatoes at any initial population density in their field. The models were first tested in a pilot project in 2010 with 3 resistant potato genotypes in (5 kg) pots at a range of 13 nematode densities. In 2011, another 8 genotypes were tested in (10 kg) pots at a range of 12 densities. The results showed that Seinhorst’s population dynamical models for nematodes with multiple generations fitted well, except in one genotype tested in 2011which lacked resistance to M. chitwoodi, and a reduction of the number of densities used seems possible. In 2012, research was initiated to investigate whether the pot size can be downscaled from 10 to 5 or even to 2 kg pots – also at 12 densities -, without loss of quality of the estimated relative susceptibility. Also, growth, yield loss and quality damage as root knot index (RKI) were assessed and compared. Genotypes 2011M1, MDG2 and cv. D{\'e}sir{\'e}e (control) were the tested potatoes. The population dynamical model fitted well for the genotypes tested. The maximum multiplications rate “a” and the maximum population density “M” at 2, 5 and 10 kg pots were estimated and used to calculate the rsa and rsM values . Despite a decrease in “a” and “M” values with increasing pot size the rsa values were relatively stable. The rsM values were a bit higher in 5 kg pots. Seinhorst yield models used to describe the fresh tuber weight also fitted well. The RKI values obtained from the three pots sizes were also stable as a quality measure for industrial processing. Overall results indicate the possibility of downscaling the resistance test for M. chitwoodi in potato in terms of pot size and number of densities. Implications of the current research in the development of a cheap and reliable resistance test will be discussed.",
author = "M.G. Teklu and T.H. Been and C.H. Schomaker",
year = "2013",
language = "English",
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booktitle = "Proceedings of the 52nd Annual meeting of Society of nematologist, July 14- 17, 2013, Knoxville, Tennessee, USA",

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Teklu, MG, Been, TH & Schomaker, CH 2013, Methodology development for partial resistance testing of potato cultivars resistant to M. chitwoodi. in Proceedings of the 52nd Annual meeting of Society of nematologist, July 14- 17, 2013, Knoxville, Tennessee, USA. Knoxville, Tennessee, USA, pp. 106-107, 52nd Annual meeting of Society of nematologist, Knoxville, Tennessee, USA, 14/07/13.

Methodology development for partial resistance testing of potato cultivars resistant to M. chitwoodi. / Teklu, M.G.; Been, T.H.; Schomaker, C.H.

Proceedings of the 52nd Annual meeting of Society of nematologist, July 14- 17, 2013, Knoxville, Tennessee, USA. Knoxville, Tennessee, USA, 2013. p. 106-107.

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

TY - CHAP

T1 - Methodology development for partial resistance testing of potato cultivars resistant to M. chitwoodi

AU - Teklu, M.G.

AU - Been, T.H.

AU - Schomaker, C.H.

PY - 2013

Y1 - 2013

N2 - M. chitwoodi was first described in 1995 in The Netherlands (Karssen, 1995). It is now listed as a quarantine organism in the EPPO region with 4 EU member states officially infested. Since 1996, research has been initiated to identify resistant genes against M. chitwoodi from wild species of tuber bearing potatoes and integrate these genes into cultivated potatoes. Currently, several breeding companies successfully produced resistant genotypes against M. chitwoodi. Parallel to this, research was started to develop a standard methodology to screen the partial resistance of these genotypes. Population dynamical models were used to estimate their level of resistance, expressed as percentage of relative susceptibility (rs). This methodology provides farmers with quantitative information on the effect of growing resistant potatoes at any initial population density in their field. The models were first tested in a pilot project in 2010 with 3 resistant potato genotypes in (5 kg) pots at a range of 13 nematode densities. In 2011, another 8 genotypes were tested in (10 kg) pots at a range of 12 densities. The results showed that Seinhorst’s population dynamical models for nematodes with multiple generations fitted well, except in one genotype tested in 2011which lacked resistance to M. chitwoodi, and a reduction of the number of densities used seems possible. In 2012, research was initiated to investigate whether the pot size can be downscaled from 10 to 5 or even to 2 kg pots – also at 12 densities -, without loss of quality of the estimated relative susceptibility. Also, growth, yield loss and quality damage as root knot index (RKI) were assessed and compared. Genotypes 2011M1, MDG2 and cv. Désirée (control) were the tested potatoes. The population dynamical model fitted well for the genotypes tested. The maximum multiplications rate “a” and the maximum population density “M” at 2, 5 and 10 kg pots were estimated and used to calculate the rsa and rsM values . Despite a decrease in “a” and “M” values with increasing pot size the rsa values were relatively stable. The rsM values were a bit higher in 5 kg pots. Seinhorst yield models used to describe the fresh tuber weight also fitted well. The RKI values obtained from the three pots sizes were also stable as a quality measure for industrial processing. Overall results indicate the possibility of downscaling the resistance test for M. chitwoodi in potato in terms of pot size and number of densities. Implications of the current research in the development of a cheap and reliable resistance test will be discussed.

AB - M. chitwoodi was first described in 1995 in The Netherlands (Karssen, 1995). It is now listed as a quarantine organism in the EPPO region with 4 EU member states officially infested. Since 1996, research has been initiated to identify resistant genes against M. chitwoodi from wild species of tuber bearing potatoes and integrate these genes into cultivated potatoes. Currently, several breeding companies successfully produced resistant genotypes against M. chitwoodi. Parallel to this, research was started to develop a standard methodology to screen the partial resistance of these genotypes. Population dynamical models were used to estimate their level of resistance, expressed as percentage of relative susceptibility (rs). This methodology provides farmers with quantitative information on the effect of growing resistant potatoes at any initial population density in their field. The models were first tested in a pilot project in 2010 with 3 resistant potato genotypes in (5 kg) pots at a range of 13 nematode densities. In 2011, another 8 genotypes were tested in (10 kg) pots at a range of 12 densities. The results showed that Seinhorst’s population dynamical models for nematodes with multiple generations fitted well, except in one genotype tested in 2011which lacked resistance to M. chitwoodi, and a reduction of the number of densities used seems possible. In 2012, research was initiated to investigate whether the pot size can be downscaled from 10 to 5 or even to 2 kg pots – also at 12 densities -, without loss of quality of the estimated relative susceptibility. Also, growth, yield loss and quality damage as root knot index (RKI) were assessed and compared. Genotypes 2011M1, MDG2 and cv. Désirée (control) were the tested potatoes. The population dynamical model fitted well for the genotypes tested. The maximum multiplications rate “a” and the maximum population density “M” at 2, 5 and 10 kg pots were estimated and used to calculate the rsa and rsM values . Despite a decrease in “a” and “M” values with increasing pot size the rsa values were relatively stable. The rsM values were a bit higher in 5 kg pots. Seinhorst yield models used to describe the fresh tuber weight also fitted well. The RKI values obtained from the three pots sizes were also stable as a quality measure for industrial processing. Overall results indicate the possibility of downscaling the resistance test for M. chitwoodi in potato in terms of pot size and number of densities. Implications of the current research in the development of a cheap and reliable resistance test will be discussed.

M3 - Abstract

SP - 106

EP - 107

BT - Proceedings of the 52nd Annual meeting of Society of nematologist, July 14- 17, 2013, Knoxville, Tennessee, USA

CY - Knoxville, Tennessee, USA

ER -

Teklu MG, Been TH, Schomaker CH. Methodology development for partial resistance testing of potato cultivars resistant to M. chitwoodi. In Proceedings of the 52nd Annual meeting of Society of nematologist, July 14- 17, 2013, Knoxville, Tennessee, USA. Knoxville, Tennessee, USA. 2013. p. 106-107