Methodologies for microbiota assessment in infancy and childhood

D. Radjabzadeh; S.R. Konstantinov; H.A. Moll; A.G. Uitterlinden; E.G. Zoetendal; R. Kraaij

doi:10.3920/978-90-8686-839-1_15

Methodologies for microbiota assessment in infancy and childhood

D. Radjabzadeh, S.R. Konstantinov, H.A. Moll, A.G. Uitterlinden, E.G. Zoetendal, R. Kraaij^*

^*Corresponding author for this work

Microbiology

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

Abstract

To design a study involving gut microbiota, it is important to realise what information is needed. The two main questions concerning microbiota are ‘Which microbes are there?’ and ‘What are they doing?’. These questions can then be addressed by several technical approaches: 16S ribosomal RNA profiling, metagenomics, metatranscriptomics, metaproteomics and metabolomics. In this chapter, we will provide an overview of the different approaches with their pros and cons. Furthermore, we will give an overview on the current status of sample collection and sample handling.

Original language	English
Title of host publication	Microbiota in health and disease: from pregnancy to childhood
Editors	Pamela D. Browne, Eric Claassen, Michael D. Cabana
Publisher	Wageningen Academic Publishers
Chapter	15
Pages	283-295
ISBN (Electronic)	9789086868391
ISBN (Print)	9789086862948
DOIs	https://doi.org/10.3920/978-90-8686-839-1_15
Publication status	Published - 14 Jul 2017

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10.3920/978-90-8686-839-1_15

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Radjabzadeh, D., Konstantinov, S. R., Moll, H. A., Uitterlinden, A. G., Zoetendal, E. G., & Kraaij, R. (2017). Methodologies for microbiota assessment in infancy and childhood. In P. D. Browne, E. Claassen, & M. D. Cabana (Eds.), Microbiota in health and disease: from pregnancy to childhood (pp. 283-295). Wageningen Academic Publishers. https://doi.org/10.3920/978-90-8686-839-1_15

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Methodologies for microbiota assessment in infancy and childhood. / Radjabzadeh, D.; Konstantinov, S.R.; Moll, H.A. et al.
Microbiota in health and disease: from pregnancy to childhood. ed. / Pamela D. Browne; Eric Claassen; Michael D. Cabana. Wageningen Academic Publishers, 2017. p. 283-295.

Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review

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AU - Konstantinov, S.R.

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AB - To design a study involving gut microbiota, it is important to realise what information is needed. The two main questions concerning microbiota are ‘Which microbes are there?’ and ‘What are they doing?’. These questions can then be addressed by several technical approaches: 16S ribosomal RNA profiling, metagenomics, metatranscriptomics, metaproteomics and metabolomics. In this chapter, we will provide an overview of the different approaches with their pros and cons. Furthermore, we will give an overview on the current status of sample collection and sample handling.

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BT - Microbiota in health and disease: from pregnancy to childhood

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Methodologies for microbiota assessment in infancy and childhood

Abstract

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