Method validation study on determination of OC-pesticides and PCBs by GC-ECD and GC-MS(/MS) in animal feeding stuffs

S.P.J. van Leeuwen, J. de Jong

Research output: Book/ReportReportProfessional

Abstract

Due to new legislation setting maximum limits for non dioxin-like polychlorinated biphenyls (ndl-PCBs), the European Commission Mandate M/523 called for modification of current EN standards for determination of PCBs and organochlorine pesticides (OCPs). This concerns the standards EN-15741:2009 (GC-MS) and EN 15742:2009 (GC-ECD). WFSR (at that time RIKILT) Wageningen University & Research was requested by the European Committee for Standardization (CEN) to adapt current standards. The modifications concern (a) reduction of the limit of quantification (LOQ) for each individual ndl-PCB congener from 5 ng/g to 0.5 ng/g and (b) extending the scope of the methods with the OCPs photoheptachlor, cis/trans nonachlor and keto-endrin. The modifications of EN 15742:2009 (GC-ECD) concern the inclusion of above mentioned additional OCPs. In addition, the determination of the ndl-PCBs is removed from this standard as GC-ECD is not capable of reaching the lowered LOQs for the ndl-PCBs. Therefore, the modified EN 15742:2009 (GC-ECD) limits to the determination of OCPs only. The modifications of EN 15741:2009 (GC-MS(/MS)) concern the inclusion of above mentioned additional OCPs, as well as the lowered LOQs for the ndl-PCBs. The revised standard for GC-ECD (EN15741) could not be evaluated due to insufficient number of participating laboratories (n=3). The extension of the standard with keto-endrin, photo-heptachlor and cis/trans-nonachlor is therefore not possible. Nevertheless the current standard remains intact for the OCPs that were included in the original standard. The standard for GC-MS(/MS) (EN15742) was successfully revised. The method is suitable for analysis of nearly all ndl-PCBs in all tested matrices. The method is also suitable for the majority of OCPs in the included feed matrices. The suitability of the standard could not be evaluated for the newly added organochlorine pesticides (endrin ketone, cis- and trans-nonachlor and photo heptachlor), mainly because only 5 out of 10 laboratories had sent in results and also because some laboratories were non-compliant.
Original languageEnglish
Place of PublicationWageningen
PublisherWageningen Food Safety Research
Number of pages26
DOIs
Publication statusPublished - Apr 2022

Publication series

NameReport / Wageningen Food Safety Research
No.WFSR 2020.001

Fingerprint

Dive into the research topics of 'Method validation study on determination of OC-pesticides and PCBs by GC-ECD and GC-MS(/MS) in animal feeding stuffs'. Together they form a unique fingerprint.

Cite this