Method of producing haploid and doubled haploid plant embryos

R. Dirks (Inventor), H. Ghislain (Inventor), J.B.M. Custers (Inventor), C.L.C. Lelivelt (Inventor), G.C. Angenent (Inventor)

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Abstract

The invention relates to a method for producing haploid plant embryos, comprising providing microspores or pollen that comprise cell division inducing molecules; pollinating an embryo sac cell, in particular an egg cell, of the plant of which the haploid embryo is to be made with the microspores or pollen; allowing the microspores or pollen to discharge the cell division inducing molecules in or in the vicinity of the embryo sac cell, in particular the egg cell, to trigger division thereof to obtain a haploid plant embryo. When doubled haploid plant embryos are to be produced doubling of the chromosome number takes place at a certain stage after pollination, in particular during cell division or after obtaining the embryo. The invention further relates to the embryos thus obtained, plants regenerated therefrom and progeny thereof.
Original languageEnglish
Patent numberWO2006128707
Priority date31/05/15
Publication statusPublished - 7 Dec 2006

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Dirks, R., Ghislain, H., Custers, J. B. M., Lelivelt, C. L. C., & Angenent, G. C. (2006). Method of producing haploid and doubled haploid plant embryos. (Patent No. WO2006128707).