C. cohnii is cultured in a suitable growth medium with acetic acid/acetate as the main carbon source. The acetate is provided, and replenished, by adding acetic acid to the growth medium in response to an increase in pH resulting from the utilisation of acetic acid/acetate by C. cohnii. The C. cohnii produces relatively high levels of docosahexaenoic acid (DHA). A stationary phase is not essential for satisfactory DHA production.
|Publication status||Published - 20 Dec 2005|
Ratledge, C., Anderson, A. J., Kanagachandran, K., Grantham, D. J., Stephenson, J. C., de Swaaf, M. E., & Sijtsma, L. (2005). Method of producing an oil including docosahexaenoic acid. (Patent No. US6977166). http://v3.espacenet.com/origdoc?DB=EPODOC&IDX=US6977166&F=0&QPN=US6977166