The present invention relates to a method of cross-linking a protein or peptide and a phenolic polymer or oligomer having substituents derived from carboxylic acids containing hydroxyl substituted phenyl groups by means of an enzyme and an oxidizing agent suitable for the enzyme in a solvent, which method comprises reacting a mixture of protein or peptide, oxidizing agent, enzyme and polymer or oligomer in the solvent, wherein the method is controlled such that in the mixture the ratio of target amino groups in the protein or peptide to hydroxyl substituted phenyl groups in the polymer or oligomer is more than equimolar. With the present method predominantly hetero-cross-linking of protein and polymer occurs.
|Publication status||Published - 9 Jan 2002|
Oudgenoeg, G., Boeriu, C. G., Hilhorst, H. M., Gruppen, H., Laane, N. C. M., & Voragen, A. G. J. (2002). Method of enzymatically cross-linking proteins and phenolic polymers. (Patent No. EP1169922). https://nl.espacenet.com/publicationDetails/originalDocument?CC=EP&NR=1169922A1&KC=A1&FT=D&ND=3&date=20020109&DB=EPODOC&locale=nl_NL