Method of enzymatically cross-linking proteins and phenolic polymers

G. Oudgenoeg; C.G. Boeriu; H.M. Hilhorst; H. Gruppen; N.C.M. Laane; A.G.J. Voragen

Method of enzymatically cross-linking proteins and phenolic polymers

G. Oudgenoeg (Inventor), C.G. Boeriu (Inventor), H.M. Hilhorst (Inventor), H. Gruppen (Inventor), N.C.M. Laane (Inventor), A.G.J. Voragen (Inventor)

Research output: Patent

Abstract

The present invention relates to a method of cross-linking a protein or peptide and a phenolic polymer or oligomer having substituents derived from carboxylic acids containing hydroxyl substituted phenyl groups by means of an enzyme and an oxidizing agent suitable for the enzyme in a solvent, which method comprises reacting a mixture of protein or peptide, oxidizing agent, enzyme and polymer or oligomer in the solvent, wherein the method is controlled such that in the mixture the ratio of target amino groups in the protein or peptide to hydroxyl substituted phenyl groups in the polymer or oligomer is more than equimolar. With the present method predominantly hetero-cross-linking of protein and polymer occurs.

Original language	English
Patent number	EP1169922
Priority date	3/07/00
Publication status	Published - 9 Jan 2002

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Cite this

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title = "Method of enzymatically cross-linking proteins and phenolic polymers",

abstract = "The present invention relates to a method of cross-linking a protein or peptide and a phenolic polymer or oligomer having substituents derived from carboxylic acids containing hydroxyl substituted phenyl groups by means of an enzyme and an oxidizing agent suitable for the enzyme in a solvent, which method comprises reacting a mixture of protein or peptide, oxidizing agent, enzyme and polymer or oligomer in the solvent, wherein the method is controlled such that in the mixture the ratio of target amino groups in the protein or peptide to hydroxyl substituted phenyl groups in the polymer or oligomer is more than equimolar. With the present method predominantly hetero-cross-linking of protein and polymer occurs.",

author = "G. Oudgenoeg and C.G. Boeriu and H.M. Hilhorst and H. Gruppen and N.C.M. Laane and A.G.J. Voragen",

year = "2002",

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day = "9",

language = "English",

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TY - PAT

T1 - Method of enzymatically cross-linking proteins and phenolic polymers

AU - Oudgenoeg, G.

AU - Boeriu, C.G.

AU - Hilhorst, H.M.

AU - Gruppen, H.

AU - Laane, N.C.M.

AU - Voragen, A.G.J.

PY - 2002/1/9

Y1 - 2002/1/9

N2 - The present invention relates to a method of cross-linking a protein or peptide and a phenolic polymer or oligomer having substituents derived from carboxylic acids containing hydroxyl substituted phenyl groups by means of an enzyme and an oxidizing agent suitable for the enzyme in a solvent, which method comprises reacting a mixture of protein or peptide, oxidizing agent, enzyme and polymer or oligomer in the solvent, wherein the method is controlled such that in the mixture the ratio of target amino groups in the protein or peptide to hydroxyl substituted phenyl groups in the polymer or oligomer is more than equimolar. With the present method predominantly hetero-cross-linking of protein and polymer occurs.

AB - The present invention relates to a method of cross-linking a protein or peptide and a phenolic polymer or oligomer having substituents derived from carboxylic acids containing hydroxyl substituted phenyl groups by means of an enzyme and an oxidizing agent suitable for the enzyme in a solvent, which method comprises reacting a mixture of protein or peptide, oxidizing agent, enzyme and polymer or oligomer in the solvent, wherein the method is controlled such that in the mixture the ratio of target amino groups in the protein or peptide to hydroxyl substituted phenyl groups in the polymer or oligomer is more than equimolar. With the present method predominantly hetero-cross-linking of protein and polymer occurs.

M3 - Patent

M1 - EP1169922

ER -

Method of enzymatically cross-linking proteins and phenolic polymers

Abstract

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