Method for the production of hydroxylated collagen-like compounds using prolyl hydroxylase from Hansenula polymorpha

E.C. de Bruin (Inventor), M.W.T. Werten (Inventor), F.A. de Wolf (Inventor)

    Research output: PatentOther research output

    Abstract

    The present invention relates to a method for the production of collagen-like compounds containing hydroxylated proline residues. Of specific interest is the production of recombinant collagen-like compounds in which hydroxylation of proline residues is achieved by a prolyl hydroxylase from a fungus, preferably a yeast, in particular Hansenula polymorpha. Also the invention concerns a method for controlling the hydroxylation of proline residues by such a prolyl hydroxylase characterised by the addition of collagen-like oligopeptides, such as gelatine hydrolysate, in particular gelatone or peptone.
    Original languageEnglish
    Patent numberWO0204615
    Priority date11/07/00
    Publication statusPublished - 17 Jan 2002

    Keywords

    • Collagens, preparation
    • Proteins
    • Hydroxylation
    • Microorganism
    • Glyoproteins
    • Gelatins, processes
    • Cryptococcus curvatus
    • Fungi
    • Kluyveromyces lactis
    • Komagataella pastoris
    • Pichia angusta
    • Saccharomyces cerevisiae

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